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Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion

A technology of DPPG-74 and Bacillus amyloliquefaciens, which is applied in the field of agricultural biology, can solve problems such as unstable performance, and achieve broad-spectrum antibacterial activity, strong resistance to stress, and stable application effects

Inactive Publication Date: 2015-05-06
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Microbial pesticides can have a certain inhibitory effect on pathogens in the soil to prevent disease, but a large number of microorganisms and environmental conditions in the soil can also affect the survival of functionally active microorganisms and affect their disease prevention efficiency. Microbial pesticides have strong regional adaptability, so many functional strains that have good disease prevention efficacy in other places are often unstable in Xinjiang

Method used

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  • Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion
  • Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion
  • Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening and identification of disease-preventing and growth-promoting bacteria DPPG-74

[0023] The screening steps are as follows:

[0024] Step 1: Sampling

[0025] Collected from the soil with severe root rot in the early spring seedling stage of processing tomato.

[0026] Step Two: Enrichment

[0027] Weigh 10g of the soil samples collected from the processing tomato production area, add 100mL of sterilized water, then add appropriate amount of glass beads and shake vigorously for 30min (to fully disperse the soil particles), let it stand for 10min, take 1mL of the supernatant and add In a Erlenmeyer flask filled with 99mL of sterilized water, carry out gradient dilution. Isolate bacteria to take 10 -4 、10 -6 concentration. Add 200 μl of each of the above dilutions to PDA, NA and Gao’s No. 1 medium plates, spread evenly with a spreader, cool and place them upside down in a 28°C incubator for cultivation, and repeat three times. After 3-7 days, si...

Embodiment 2

[0044] Example 2 Bacillus amyloliquefaciens DPPG-74 has disease-preventing and growth-promoting characteristics of processed tomato

[0045] The fermented stock solution of the DPPG-74 strain (10 11 cfu / ml) soak processed tomato seeds, soak for 12h and then dry for later use. Then the treated tomato seeds were evenly sown into the nutrient bowl, and three controls were set up: CK 1 (Soaking seeds in sterilized culture solution, diseased soil), CK 2 (soak seeds in sterilized culture solution, the soil is sterilized soil), CK 3 (Chemical pesticides PCNB, metalaxyl, Weifu seed coating agent and Dikexon soaked processed tomato seeds, diseased soil). 20 seeds per pot, three repetitions per treatment, regular watering: 30 days after seed emergence, the seedling rate, growth and disease incidence were investigated. Perform statistical analysis on the results.

[0046] Table 3 Determination of the control effect of antagonistic bacteria on tomato Pythium root rot

[0047]

[...

Embodiment 3

[0050] Embodiment 3 Bacillus amyloliquefaciens DPPG-74 is to Pythium mycelia morphology influence

[0051] Add 20mL of PD culture solution to a sterile petri dish with a diameter of 90mm, insert a piece of bacteria sheet with a diameter of 8mm, and place it at a constant temperature at 6°C for 2 days. After 24 hours of treatment with the bacteria, the mycelium was picked to observe the shape of the mycelium under a microscope, and compared with the shape of the mycelium that had not been treated with the disease-preventing and growth-promoting bacteria. Each treatment was repeated four times, and water control was set.

[0052] Preliminary study on the antibacterial mechanism of Pythium melon and fruit mycelium will be treated with the biocontrol bacteria DPPG-74. Such as figure 1 As shown, the influence of biocontrol bacteria on the mycelium of Pythium melon and fruit can be observed under the microscope, and the mycelium morphology of Pythium melon and fruit after 48 hours...

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Abstract

The invention discloses bacillus amyloliquefaciens DPPG-74. The bacillus amyloliquefaciens DPPG-74 has an accession number of CGMCC No.9396. The bacillus amyloliquefaciens DPPG-74 is screened from rhizosphere soil in a processing tomato severe root rot zone and roots suffering from severe root rot, is gram-positive bacterium, has wide spectrum bacteriostatic activity and very strong stress resistance, can multiply and colonize on surfaces of fungal pathogenic mycelium, effectively inhibits pathogen growth, controls diseases, has high toxicity to pathogens and can effectively prevent and control Xinjiang processing tomato root rot in a seedling stage. Plants treated by the fermentation bacteria liquid has height, fresh weight and dry weight respectively improved by 50.39%, 91.98% and 40.41%, prevention and control rate is 67.74% and nutrient biomass of the plants is effectively improved. The bacillus amyloliquefaciens DPPG-74 allows simple culture conditions, can be preserved easily, is environmentally friendly and has good development and popularization application potential.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology. More specifically, the present invention relates to a Bacillus amyloliquefaciens strain DPPG-74 and its application in disease prevention and growth promotion. Background technique [0002] Tomato, also known as tomato, is native to Peru and Ecuador in South America. Because of its wide adaptability, easy cultivation, high yield and long supply period, as well as its rich nutrition and unique flavor, it can be processed into tomato paste, tomato juice, diced tomato, canned tomato and tomato paste in addition to being eaten fresh. It also has the effect of preventing cancer, and has become an important functional food. In the process of tomato planting, diseases are very common. There are nearly a hundred kinds of diseases that can harm tomato production. The common ones are early blight, leaf mold, fusarium wilt, root rot: bacterial spot, scab, canker , navel rot, mosaic virus disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12P21/04C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 李春武占省凃亮樊艳爽何艳慧
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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