cyhv‑2 monoclonal antibody, hybridoma cells and applications

Abstract

The invention discloses a CyHV-2 (Cyprinid Herpesvirus 2) monoclonal antibody, a hybridoma cell and an application of the CyHV-2 monoclonal antibody. The CyHV-2 monoclonal antibody disclosed by the invention is secreted from a hybridoma cell GFHNV-6G7 with the collection number of CCTCC No. C2013114 or hybridoma cell GFHNV-3B5 with the collection number of CCTCC No. C2013115. The CyHV-2 monoclonal antibody disclosed by the invention has good specificity and potency; by producing rapid immunodetection test paper from the CyHV-2 monoclonal antibody, the speed and efficiency of detection can be increased, and field detection on CyHV-2 can be realized. The CyHV-2 monoclonal antibody disclosed by the invention makes up the blank in immunodetection on the CyHV-2 and lays a foundation for immunological research on the CyHV-2.

Description

technical field [0001] The present application relates to the field of monoclonal antibodies, in particular to the monoclonal antibody for CyHV-2, the hybridoma cells secreting the monoclonal antibody, and the application of the monoclonal antibody. Background technique [0002] Cyprinid herpesvirus 2 (CyHV-2 for short) is the pathogen of herpes virus hematopoietic organ necrosis, which can cause a large number of deaths of farmed goldfish and crucian carp. From 2011 to 2012, the disease broke out in cultured crucian carp in central my country, resulting in extremely high mortality (Wang et al., 2012). In 2011, CyHV-2 was detected in goldfish exported from my country to Malaysia. In the same year, a large number of crucian carp died in an outbreak in Jiangsu Province. The assay was successful and the virus particles were observed in the spleen and kidney. It can be seen that herpes virus hematopoietic organ necrosis has been introduced into my country, and has seriously aff...

AI Technical Summary

Problems solved by technology

Cell culture isolation techniques are the most accurate diagnostic method, however, studies have found that CyHV-2 is difficult to proliferate in cell lines commonly used for fish virus isolation

In terms of molecular detection, especially the PCR method is currently a more accurate method for detecting CyHV-2 infection because it can detect a very small amount of viral DNA in tissues; Inconvenience

Electron microscope observation is the most direct method, but it also requires special testing equipment, and it is not convenient for on-site rapid diagnostic testing

[0004] The existing detection methods for goldfish herpesvirus hematopoietic necrosis are mainly PCR, histopathology and other methods, and the detection methods are relatively simple; while the immunological research is relatively lacking or lagging behind, there is no immunological detection method for this disease

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