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Method for measuring content of amino acid in amino acid chromium complex

A technology of chromium chelate and determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex amino acid derivatization steps and expensive derivatization reagents, achieve good accuracy performance, strong method applicability, and rapid determination Effect

Inactive Publication Date: 2015-04-29
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the derivatization steps for amino acids are complex and the derivatization reagents are relatively expensive

Method used

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  • Method for measuring content of amino acid in amino acid chromium complex
  • Method for measuring content of amino acid in amino acid chromium complex
  • Method for measuring content of amino acid in amino acid chromium complex

Examples

Experimental program
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Embodiment 1

[0065] Embodiment 1: the establishment of test method

[0066] This embodiment takes methionine, phenylalanine, glycine and threonine as examples to establish the test method, and calculates the stability, repeatability and standard addition recovery steps of the established test method with the chromium chelate of the corresponding amino acid as follows:

[0067] (1) Prepare standard solution:

[0068] Take 0.1019g methionine standard product, 0.1000g glycine standard product, 0.0978g threonine standard product and 0.1025g phenylalanine standard product respectively in a 100mL volumetric flask, and dilute to 0.05mol / L hydrochloric acid solution to Scale, shake well as the reference standard solution for subsequent use, the concentration of the reference standard solution is recorded as X; the reference standard solution is diluted with 0.05mol / L hydrochloric acid solution as a solvent, and the concentration is 0.01X , 0.02X, 0.05X, 0.08X, 0.1X, 0.2X, 0.4X, 0.5X, 1.0X, stand...

Embodiment 2

[0117] The assay method of methionine in embodiment 2 methionine chromium chelates

[0118] The present embodiment adopts the test method established in Example 1 to measure the methionine in the methionine chelate. The theoretical content of methionine in the methionine chromium chelate described in the present embodiment is 89.59%, which specifically includes the following steps:

[0119] (1) Preparation of the test sample solution: Accurately weigh 30.3 mg of chromium methionine chelate and place it in a 50 mL volumetric flask, add 0.05 mol / L hydrochloric acid solution to completely dissolve the solid sample, dilute to the mark, shake well, and use 0.45 The sample solution to be tested was obtained by filtering with a μm filter membrane;

[0120] (2) Inject the test sample solution obtained in step (1) into a high-performance liquid chromatograph for analysis, and the analysis conditions are:

[0121] ZORBAX Extend-C18 liquid chromatography column (150×4.6mm, 5μm); column ...

Embodiment 3

[0124] The assay method of glycine in the chromium glycine chelate of embodiment 3

[0125] The present embodiment adopts the test method established in Example 1 to measure the glycine in the glycine chelate, and the theoretical content of glycine in the glycine chromium chelate described in the present embodiment is 81.04%, specifically comprising the following steps:

[0126] (1) Preparation of test sample solution: Accurately weigh 23.8 mg of chromium glycinate chelate and place it in a 50 mL volumetric flask, add 0.05 mol / L sulfuric acid solution to completely dissolve the solid sample, dilute to the mark, shake well, and use 0.45 The sample solution to be tested was obtained by filtering with a μm filter membrane;

[0127] (2) Inject the test sample solution obtained in step (1) into a high-performance liquid chromatograph for analysis, and the analysis conditions are:

[0128] ZORBAX Extend-C18 liquid chromatography column (150×4.6mm, 5μm); column temperature 35°C; mob...

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Abstract

The invention relates to a method for measuring the content of an amino acid in an amino acid chromium complex. The method comprises the following steps: (1) adding an inorganic acid solution into a weighed amino acid standard product, thereby preparing a standard solution; (2) adding the inorganic acid solution into a weighed amino acid chromium complex test sample to be tested so as to dissolve the sample, thereby obtaining a test sample solution; (3) respectively performing high performance liquid chromatography on the standard product solution and the test sample solution, thereby obtaining the content of the amino acid in the amino acid chromium complex by using an external standard method, wherein the analysis conditions are that a C18 liquid phase chromatographic column is adopted as a chromatographic column, and a phosphate solution is adopted as the flow phase. The method for measuring the content of the amino acid in the amino acid chromium complex is simple in sample pretreatment process, simple and convenient to operate, capable of rapidly measuring the content of the amino acid in amino acid chromium complex and relatively high in sensitivity, accuracy, stability and reproducibility, and a reliable and effective method is provided for quality detection on the amino acid chromium complex.

Description

technical field [0001] The invention relates to a quantitative determination method of amino acid trace element chelate, in particular to a method for determining amino acid content in amino acid chromium chelate by high performance liquid chromatography. Background technique [0002] Amino acid trace element chelate is a compound with cyclic results obtained by chelating one or more amino acid groups with trace metal ions. It organically combines amino acids and trace elements essential to mammals, and has a stable Physical and chemical properties and high biological activity. Amino acid trace element chelate has become a highly efficient trace element nutrition enhancer and is widely used in medicine, food and animal husbandry. [0003] For example, trivalent chromium Cr (III) is one of the trace elements necessary for human and animals to maintain normal glucose metabolism and lipid metabolism. As an organic trivalent chromium product, amino acid chromium chelate has an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 肖清贵唐海燕徐红彬张懿
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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