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A kind of preparation method of clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody

A technology of clenbuterol hydrochloride and biomimetic antibodies, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of template molecule leakage and low specificity of biomimetic antibodies, so as to improve specificity and avoid leakage problems , to ensure the effect of mass transfer speed

Active Publication Date: 2017-09-15
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies of the prior art, the present invention proposes a new preparation method of CLB molecularly imprinted polymer biomimetic antibody, aiming at solving the problems of low specificity and template molecular leakage of existing CLB molecularly imprinted polymer biomimetic antibody

Method used

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  • A kind of preparation method of clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody
  • A kind of preparation method of clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody
  • A kind of preparation method of clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody

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preparation example Construction

[0032] The preparation method of this CLB molecularly imprinted polymer biomimetic antibody of the present invention, its steps are as follows:

[0033] (1) Preparation of CLB derivatives:

[0034] Clenbuterol hydrochloride was dissolved in dichloromethane solvent, triethylamine was added and methacryloyl chloride was added dropwise in the dichloromethane solution, and the mixture was reacted overnight at room temperature. After the reaction, wash the reaction solution with saturated sodium bicarbonate solution, separate the organic phase, wash the water phase twice with dichloromethane, combine the organic phases, dry over anhydrous sodium sulfate, and remove the organic solvent under reduced pressure with a rotary evaporator. The residue was purified with a silica gel column using petroleum ether-ethyl acetate mixed solvent as eluent to obtain CLB derivatives.

[0035] (2) Preparation of CLB molecularly imprinted polymer biomimetic antibody:

[0036] Dissolve the clenbuterol...

Embodiment 1

[0046] A method for preparing a CLB molecularly imprinted polymer biomimetic antibody, the steps of which are as follows:

[0047] (1) Preparation of CLB derivatives:

[0048] Dissolve 10 mmol of clenbuterol hydrochloride in 50 mL of dichloromethane, add 40 mmol of triethylamine to the solution, then dropwise add 20 mmol of methacryloyl chloride, and react overnight at room temperature. After the reaction, wash with saturated sodium bicarbonate solution, separate the organic phase, wash the water phase twice with dichloromethane, combine all the separated organic phases, dry with anhydrous sodium sulfate, and use a rotary evaporator to The organic solvent was removed under reduced pressure, and the residue was purified with petroleum ether-ethyl acetate (3:1, V:V) with a silica gel column (silica gel diameter 200-300 mesh) to obtain the product CLB derivative.

[0049] (2) Preparation of CLB molecularly imprinted polymer biomimetic antibody:

[0050] Take 1 mmol of the CLB d...

Embodiment 2

[0052] A method for preparing a CLB molecularly imprinted polymer biomimetic antibody, the steps of which are as follows:

[0053] (1) Preparation of CLB derivatives:

[0054] Dissolve 10 mmol of clenbuterol hydrochloride in 55 mL of dichloromethane, add 35 mmol of triethylamine to the solution, then dropwise add 25 mmol of methacryloyl chloride, and react overnight at room temperature. After the reaction, wash with saturated sodium bicarbonate solution, separate the organic phase, wash the water phase twice with dichloromethane, combine all the separated organic phases, dry with anhydrous sodium sulfate, and use a rotary evaporator to The organic solvent was removed under reduced pressure, and the residue was purified with petroleum ether-ethyl acetate (3:1, V:V) with a silica gel column (silica gel diameter 200-300 mesh) to obtain the product CLB derivative.

[0055] (2) Preparation of CLB molecularly imprinted polymer biomimetic antibody:

[0056] Take 1 mmol of the CLB d...

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Abstract

The invention relates to a preparation method of clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody, which aims to solve the problems of low specificity and template molecule leakage of existing CLB molecularly imprinted polymer biomimetic antibody. The steps of the method are as follows: (1) preparing CLB derivatives; (2) preparing CLB molecularly imprinted polymer bionic antibodies. The CLB molecularly imprinted polymer biomimetic antibody prepared by the present invention adopts covalent bond polymerization, non-covalent bond way to adsorb the target substance, has stable physical and chemical properties, strong specificity, fast adsorption rate, no leakage of CLB molecules, and is suitable for bionics Establishment of immunoassay detection method.

Description

technical field [0001] The invention relates to a preparation method of a biomimetic antibody, in particular to a preparation method of a covalent clenbuterol hydrochloride molecularly imprinted polymer biomimetic antibody. Background technique [0002] Clenbuterol (CLB) is an adrenal nerve stimulant, which has been clinically used to prevent and treat bronchial asthma, chronic bronchitis and other diseases. CLB is a kind of "lean meat extract" with high bioavailability, which can change the metabolic pathways in animals, promote protein synthesis in muscles and bones, accelerate growth, improve carcass quality, and increase lean meat percentage. However, CLB is easy to accumulate in the animal body, and the residual amount in the lung, liver, kidney and other organs, muscle and fat tissue is relatively large, and the residual time is relatively long, and it can accumulate in the human body through the food chain, causing toxic and side effects on the body. The main manifes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/34C08F222/14C08J9/26B01J20/26B01J20/30
Inventor 汤轶伟兰建兴高雪于志鹏刘秀英励建荣张德福吕长鑫
Owner BOHAI UNIV
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