Tissue-engineered epidermis and preparation method thereof

Abstract

The invention belongs to the field of histocytology and tissue engineering and discloses a tissue-engineered epidermis with hypodermal cells and a preparation method thereof. The method for preparing the tissue-engineered epidermis with hypodermal cells comprises the following steps: obtaining epidermis cells; inoculating the obtained epidermis cells, culturing the cells until the density is 80-100 percent by using an epidermis culture medium, replacing the medium into a mixed culture medium, culturing for 16-24 hours, and collecting the epidermis cell layer, thereby obtaining the tissue-engineered epidermis piece; transferring the obtained tissue-engineered epidermis onto a semi-permeable polymer or a silicone mold substrate or a petrolatum gauze, thereby obtaining a film with the epidermis cell layer; and culturing the hypodermal cells, paving the obtained hypodermal cells on the epidermis cell layer with the film of the epidermis cell layer, and culturing for 1-2 hours, thereby obtaining the product. The preparation method provided by the invention is easy to operate and short in period, the tissue-engineered epidermis can be mutually stimulated and induced with receptor skin tissues, and formation of a complete epidermal structure of the receptor is promoted.

Description

technical field [0001] The invention belongs to the fields of histocytology and tissue engineering, in particular to a tissue engineering epidermis with dermal cells and a preparation method thereof. Background technique [0002] The skin is the largest organ in the human body. It undertakes functions such as protecting the body, perspiration, and feeling cold, heat, and pressure, and protects various tissues and organs in the body from physical, mechanical, chemical, and pathogenic microorganisms. Genetic diseases, burns, chronic skin trauma (such as diabetes), vitiligo, albinism and many other reasons can cause damage to the skin, affect the appearance of the skin, leave scars on the skin and even lose its physiological function. When the skin is seriously damaged, such as deep and large-scale burns, the skin can no longer recover by itself, and will lose its resistance to bacteria, causing a large number of bacteria to invade the human body through the skin, which can eas...

AI Technical Summary

Problems solved by technology

However, the current preparation method of tissue-engineered full-thickness skin is complicated and requires a long time of culture to form the skin structure, which seriously limits its promotion and application.

In addition, artificial dermis is also used clinically, but because it does not contain active cells and no epidermis can form normal skin, its application is limited.

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