Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ganoderma lucidum compounds and medicine composition thereof, as well as preparation method and application of compounds

A compound and composition technology, applied in the field of pharmaceutical technology and food, can solve the problem of anti-chronic kidney disease with no known compound

Inactive Publication Date: 2015-04-08
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
View PDF5 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no relevant report on the compound involved in the present invention and its anti-chronic kidney disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ganoderma lucidum compounds and medicine composition thereof, as well as preparation method and application of compounds
  • Ganoderma lucidum compounds and medicine composition thereof, as well as preparation method and application of compounds
  • Ganoderma lucidum compounds and medicine composition thereof, as well as preparation method and application of compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Separation and purification of compounds:

[0027]Take Chizhi, grind it, and extract it with 95% ethanol under reflux for 2 hours, combine the extracts and recover the solvent under reduced pressure to obtain a crude extract, suspend the crude extract in water, then extract three times with an equal volume of ethyl acetate, combine the extracts, and reduce Concentrate under reduced pressure to obtain ethyl acetate extract, which is subjected to silica gel 200-300 mesh column chromatography, chloroform-methanol system gradient (99:1, 98:2, 97:3, 96:4, 95:5, 94: 6, 93:7, 92:8, 90:10, 85:15, 80:20, 50:50) elution, each solvent gradient was 1.5 times the column volume, and 7 combined fractions were collected according to 500mL each . Among them, component 3 (90 g) was eluted by MCI gel CHP 20P column chromatography with methanol-water (10:90, 30:70, 50:50, 70:30, 80:20) to obtain components 3.1-3.3). Component 3.2 (2.5g) was eluted with Sephadex LH-20 (methanol), develope...

Embodiment 2

[0041] Any one of the compounds in Example 1 or compound 3 or 4 can be made into an injection solution after adding water for injection according to the conventional method, finely filtering, potting and sterilizing.

Embodiment 3

[0043] Dissolve any one of the compounds in Example 1 or compound 3 or 4 in sterile water for injection, filter through a sterile funnel, sub-package, freeze-dry at low temperature, and aseptically melt seal to obtain a powder injection.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides ganoderma lucidum compounds and a medicine composition thereof, as well as a preparation method and an application of the compounds. Four pairs of optical enantiomers are separated and identified from ganoderma lucidum, wherein two pairs are new compounds which are named as L-ganoderma lucidum or R-ganoderma lucidum E and F respectively. The new compounds show an application prospect in preparation of medicines and foods resisting diabetic nephropathy or chronic nephrosis because of being capable of remarkably inhibiting kidney mesangial cells induced by high glucose from generating inflammatory factors or extracellular matrixes.

Description

Technical field: [0001] The invention belongs to the field of pharmaceutical technology and food, and specifically relates to chizhi compounds and their pharmaceutical compositions, their preparation methods, and their application in the preparation of medicines or health food for treating diabetic nephropathy or chronic kidney disease. Background technique: [0002] The harm of chronic kidney disease, the pathological mechanism and the method and principle of drug research are all clearly described by the inventor in the published Chinese patent (publication number: CN 102153630A). Chronic kidney disease lacks of effective treatment drugs so far. Studies have shown that the expression of monocyte chemoattractant (MCP-1), pro-inflammatory cytokines (such as IL-6) and extracellular matrix (such as collagen IV, fibronectin) is increased in chronic kidney disease including diabetic nephropathy. Therefore, compounds that inhibit these factors may be beneficial in chronic kidney...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/58C07D307/60A61K31/365A61P3/10A61P13/12A23L1/29A23L33/00
Inventor 程永现罗奇邸磊吕青
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com