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Method for extracting astaxanthin from fresh shrimp shell

A technology of astaxanthin and fresh shrimp shells, applied in the direction of organic chemistry, etc., can solve the problems of affecting the extraction rate of astaxanthin, producing waste acid and waste lye, and high cost, so as to achieve easy promotion and application, low cost, and improved The effect of extraction rate

Inactive Publication Date: 2015-04-08
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods both use protein enzymatic hydrolysis technology and alkali treatment technology, which will produce waste acid and waste lye, the cost is high, and the extraction process needs to be heated, which is likely to affect the extraction rate of astaxanthin

Method used

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  • Method for extracting astaxanthin from fresh shrimp shell

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Take 25 g of fresh Penaeus chinensis shells and boil them in boiling water for 1 min, then remove and drain;

[0025] (2) After draining, put the shrimp shells in the refrigerator at -18℃ for 5 minutes, and immediately crush them with a plant grinder to obtain shrimp shell powder;

[0026] (3) Prepare the ethyl acetate-BHT extract with 250 mL ethyl acetate and 0.25 g 2,6-di-tert-butyl-4-methylphenol (BHT), place it in a stoppered Erlenmeyer flask, and add it to step (2) ) The crushed shrimp shell powder was extracted for 5 min under the auxiliary condition of ultrasonic power of 300 W to obtain the extract. The extract was concentrated under reduced pressure with a rotary evaporator (the temperature of the water bath of the rotary evaporator was 45℃) and then placed in- Temporarily store in the refrigerator at 18 ℃ to collect the shrimp shell residue after ultrasonic extraction;

[0027] (4) Put the shrimp shell residue collected in step (3) into a refrigerator at -18 ℃ f...

Embodiment 2

[0032] (1) Take 25 g of fresh Penaeus chinensis shells and boil them in boiling water for 5 minutes, then remove and drain;

[0033] (2) After draining, put the shrimp shells in the refrigerator at -18 ℃ for 10 minutes, and immediately crush them with a plant grinder after taking them out to obtain shrimp shell powder;

[0034] (3) Prepare the dichloromethane-BHT extract with 333 mL methanol, 167 mL dichloromethane and 0.5 g 2,6-di-tert-butyl-4-methylphenol (BHT) and place it in a stoppered Erlenmeyer flask, and Add the crushed shrimp shell powder in step (2), extract 15 min under the auxiliary condition of ultrasonic power of 400 W to obtain the extract, and use the rotary evaporator (the water bath temperature of the rotary evaporator is 45 ℃) to concentrate under reduced pressure Then put it in the refrigerator at -18 ℃, and collect the shrimp shell residue after ultrasonic extraction;

[0035] (4) Put the shrimp shell residues collected in step (3) in the refrigerator at -18 ℃ f...

Embodiment 3

[0040] (1) Take 25 g of fresh Penaeus chinensis shells and boil them in boiling water for 8 minutes, then remove and drain;

[0041] (2) After draining, put the shrimp shells in the refrigerator at -18℃ for 15 minutes, and immediately crush them with a plant grinder after taking them out to obtain shrimp shell powder;

[0042] (3) Prepare the dichloromethane-BHT extract with 750 mL of dichloromethane and 0.75 g of 2,6-di-tert-butyl-4-methylphenol (BHT), place it in a stoppered Erlenmeyer flask, and add it to step (2 ) The crushed shrimp shell powder was extracted for 25 min under the auxiliary conditions of 500 W power to obtain the extract. The extract was concentrated under reduced pressure with a rotary evaporator (the temperature of the water bath of the rotary evaporator was 45 ℃) and then placed at -18 Temporarily store in the refrigerator at ℃ to collect the shrimp shell residue after ultrasonic extraction;

[0043] (4) Freeze in a refrigerator at -18 ℃ for 2 h, then take it...

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Abstract

The invention discloses a method for extracting astaxanthin from a fresh shrimp shell. The method comprises the following steps: boiling fresh shrimp shell with boiling water, fishing out, draining, putting into a refrigerator, freezing, taking out, and crushing with a plant crusher; extracting with an organic solvent under an ultrasonic-assisted condition, so as to obtain an extract liquid; carrying out vacuum concentration, putting a concentrated liquid into the refrigerator, and storing; collecting the extracted shrimp shell residue, freezing in the refrigerator, taking out and thawing at a room temperature, and repeating the freezing-thawing process; mixing the repeated frozen and thawed shrimp shell residue with the organic solvent, stirring, extracting, and carrying out vacuum suction filtration, so as to obtain an extract liquid I and shrimp shell filter residues; repeatedly extracting the shrimp shell filter residues, so as to obtain an extract liquid II; collecting the extract liquid I and the extract liquid II by virtue of a rotary evaporator for vacuum concentration; and mixing the obtained concentrated liquid with the concentrated liquid of the extract liquid, and carrying out freeze-drying, so as to obtain an astaxanthin crude extract. The method has the advantages of being simple to operate, low in cost, and convenient to popularize and apply; the extraction process can be carried out at room temperature; and high extraction rate of the astaxanthin is ensured.

Description

Technical field [0001] The invention relates to a method for extracting astaxanthin, in particular to a method for extracting astaxanthin from fresh shrimp shells. Background technique [0002] Astaxanthin (Astaxanthin) is a keto carotenoid with the chemical name 3,3'-dihydroxy-4,4'-β,β'-carotene, which is widely found in aquatic animals such as shrimp and crab And salmon. Shrimp shell pigments are mainly composed of free astaxanthin and astaxanthin esters, accounting for about 63.5%-92.2% of the total carotenoids in shrimp shells. [0003] As the astaxanthin in shrimp shells mainly exists in the form of astaxanthin-protein complex, and the astaxanthin bound to astaxanthin is water-soluble, and the efficiency of direct solvent extraction is low. Therefore, some physical or After chemical methods destroy the astaxanthin-protein composite structure, solvent extraction is used to extract astaxanthin from shrimp shells. Alkaline extraction is a commonly used method for extracting sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 李秀霞励建荣孙协军蔡路昀仪淑敏张丽华
Owner BOHAI UNIV
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