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Preparation, use and method of biochar-immobilized composite pollution-degrading bacteria particles

A technology of complex pollution and degrading bacteria, which is applied in the direction of biochemical equipment and methods, immobilized enzymes, restoration of contaminated soil, etc., can solve the problems that soil pollution and failure cannot be fundamentally eliminated, and achieve continuous in-situ repair effect Long-term enhancement, low cost, good effect

Active Publication Date: 2017-06-13
江苏诺丽慧农农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These technologies all utilize the adsorption function of biochar to pollutants. In fact, the adsorption of biochar to pollutants is essentially a kind of physical adsorption. The use of biochar in soil not only has the problem of failure after adsorption saturation, but also, Like conventional passivators, biochar that adsorbs pollutants in the soil also has the risk of being re-released as a soil "time bomb" when environmental conditions change. Therefore, soil pollution cannot be fundamentally eliminated.

Method used

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  • Preparation, use and method of biochar-immobilized composite pollution-degrading bacteria particles
  • Preparation, use and method of biochar-immobilized composite pollution-degrading bacteria particles
  • Preparation, use and method of biochar-immobilized composite pollution-degrading bacteria particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The technological process of biochar immobilization compound pollution degrading bacteria repairing polycyclic aromatic hydrocarbon contaminated soil method in this implementation example is as follows figure 1 As shown, the specific steps of using rice husk biochar to treat PAH-contaminated soil are as follows:

[0035] Preparation of biochar: under the condition of 350°C, use rice husks to dry and pulverize under anaerobic conditions, use a carbonization furnace to pyrolyze for 4 hours to prepare biochar, grind, and pass through an 80-mesh sieve;

[0036] Selection of degrading microorganisms: the selected strain was Enterobacter cloacae strain CCTCC NO:M 2012240, and the strain used was a heavy metal-resistant polycyclic aromatic hydrocarbon degrading strain purchased from China Center for Type Culture Collection.

[0037] Preparation of biochar-immobilized microorganisms: add 20g PVA and 2g SA to 200mL distilled water and soak overnight, then stir at room temperatur...

Embodiment 2

[0051] The technological process of the biochar-immobilized microbial remediation method for PAH-contaminated soil in this implementation example is as follows: figure 1 As shown, the specific steps of using cow dung biochar to treat PAH-contaminated soil are as follows:

[0052] Preparation of biochar: At a temperature of 350°C, dry the pulverized material with cow dung under anaerobic conditions, use a carbonization furnace to pyrolyze for 4 hours to prepare biochar, grind it, and pass through an 80-mesh sieve;

[0053] Selection of degrading microorganisms: the selected strain was Enterobacter cloacae strain CCTCC NO:M 2012240, and the strain used was a heavy metal-resistant polycyclic aromatic hydrocarbon degrading strain purchased from China Center for Type Culture Collection.

[0054] Preparation of biochar-immobilized microorganisms: add 20g PVA and 2gSA to 200mL distilled water and soak overnight, then stir at room temperature for 15 minutes, heat to 95°C through a wat...

Embodiment 3

[0067] This embodiment is a variation example of embodiment 1, and the specific technical scheme is the same as that of embodiment 1, and the changes only lie in:

[0068] (1) In the preparation of biochar, the temperature is 300° C., the time is 5 hours, and the filter adopts a 100-mesh sieve;

[0069] (2) In biochar-immobilized microorganisms, the concentration of PVA is 5% (m / v), and the concentration of SA is 0.5% (m / v); the mass ratio of bacteria to carrier sol is 1:100; crosslinking agent CaCl in solution 2 The concentration is 20g / L;

[0070] (3) The weight ratio of biochar-immobilized compound pollution-degrading bacteria particles to polycyclic aromatic hydrocarbon compound-contaminated soil in coking plant is 0.5:100.

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Abstract

The invention discloses the preparation, application and use method of biochar immobilized composite pollution-degrading bacteria particles; the preparation method comprises: thermally cracking air-dried and pulverized agricultural and forestry waste and animal manure, grinding and sieving to obtain biochar; The strains of complex pollution-degrading bacteria are cultured in a liquid medium. After the strains grow to the logarithmic growth phase, the degrading bacteria are enriched by centrifugation to obtain the complex pollution-degrading bacteria cells; the complex pollution-degrading bacteria cells are added to the biological Charcoal, biochar adsorbed with composite pollution-degrading bacteria; adding biochar adsorbed with composite pollution-degrading bacteria to carrier sol to obtain composite sol; dropping composite sol into cross-linking liquid, cross-linking and fixing at low temperature, and drying. The biochar-immobilized composite pollution degrading bacteria of the present invention has good biological activity and chemical stability, can effectively remove polycyclic aromatic hydrocarbons in composite contaminated soil, accelerate the recovery of soil functional microorganisms, has low cost, simple preparation process, and obvious remediation effect on contaminated soil .

Description

technical field [0001] The invention relates to the field of biochar-immobilized microbial remediation pollution, in particular to the preparation, use and use method of biochar-immobilized composite pollution-degrading bacteria particles. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) is one of the typical persistent organic pollutants in soil, which has a strong "three-caused" effect and seriously threatens the ecological security of soil and human health. Therefore, the remediation of soil PAHs pollution has been It has become a hot issue of common concern at home and abroad. As a cost-effective means of removing organic pollution, microbial remediation has become the most potential treatment method at present, especially immobilized microbial technology provides a new way for the remediation of soil PAHs pollution. However, the current soil microbial remediation technology still has the disadvantages of low concen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/00B09C1/10
Inventor 刘亮沈国清陈平付盼李蓝青
Owner 江苏诺丽慧农农业科技有限公司
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