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A kind of Yunzhi polysaccharide extract and its preparation method and application

A technology of versicolor polysaccharides and extracts, applied in the biological field, to achieve the effects of precise ingredients, shortened preparation cycle, and good solubility

Active Publication Date: 2017-11-17
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant literature report on the research on the blood lipid-lowering effect of Yunzhi liquid submerged fermentation

Method used

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  • A kind of Yunzhi polysaccharide extract and its preparation method and application
  • A kind of Yunzhi polysaccharide extract and its preparation method and application
  • A kind of Yunzhi polysaccharide extract and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0058] (1) Coriolus versicolor (Coriolus versicolor) is cultured on a slant or flat plate, liquid shaking culture and liquid submerged fermentation culture to obtain mycelium and fermentation broth. The specific methods are as follows:

[0059] ①Slant or plate culture: Inoculate the mycelium block of the activated Yunzhi slant parent species into a new slant solid medium, culture at 24°C for 10 days, and store at 4°C for later use.

[0060] ②Level-1 liquid strain culture: Take 200mL liquid fermentation medium and put it into a 500mL Erlenmeyer flask, sterilize at 121°C for 20min, and insert 4 soybean-sized mycelia blocks of Versicolor versicolor parent species; let stand at room temperature for 24h, and wait for After the wounds of the bacteria block are healed, culture at 27°C, rotate at 150rpm, avoid light, and shake at constant temperature for 6 days to obtain the first-grade seed liquid;

[0061] ③Cultivation of secondary liquid strains: 50L seed tanks are filled with 35L ...

Embodiment 2

[0070] (1) Coriolus versicolor (Coriolus versicolor) obtains mycelium and fermented liquid through slant or plate strain culture, liquid shaking culture and liquid submerged fermentation culture, and the specific methods are as follows:

[0071] ① Slant or plate culture: Inoculate the mycelium block of the activated Yunzhi slant parent species into a new slant solid medium, culture at 25°C for 12 days, and store at 4°C for later use.

[0072] ②Level-1 liquid strain culture: Take 200mL liquid fermentation medium and put it into a 500mL Erlenmeyer flask, sterilize at 121°C for 20min, and insert 6 soybean-sized mycelia pieces of Versicolor versicolor; let it stand at room temperature for 24h, and wait for After the wounds of the bacteria block are healed, culture at 27°C, rotate at 150rpm, avoid light, and shake at constant temperature for 6 days to obtain the first-grade seed liquid;

[0073] ③Cultivation of secondary liquid strains: 50L seed tanks are filled with 35L of liquid ...

Embodiment 3

[0082] (1) Coriolus versicolor (Coriolus versicolor) obtains mycelium and fermented liquid through slant or plate strain culture, liquid shaking culture and liquid submerged fermentation culture, and the specific methods are as follows:

[0083] ①Slant or plate strain culture: Inoculate the mycelium block of the activated Yunzhi slant parent species into a new slant solid medium, culture at 27°C for 15 days, and store at 4°C for later use.

[0084] ②Level-1 liquid strain culture: Take 200mL liquid fermentation medium and put it into a 500mL Erlenmeyer flask, sterilize at 121°C for 20min, and insert 5 soybean-sized mycelium blocks of Versicolor versicolor parent species; let it stand at room temperature for 24h, and wait for After the wounds of the bacteria block are healed, culture at 27°C, rotate at 150rpm, avoid light, and shake at constant temperature for 6 days to obtain the first-grade seed liquid;

[0085] ③Cultivation of secondary liquid strains: 50L seed tanks are fill...

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Abstract

The invention relates to the field of biotechnology, in particular to a polysaccharide extract from versicolor versicolor and its preparation method and application. In the present invention, after submerged fermentation is used to cultivate Yunzhi mycelium, the fermentation product is separated into mycelium and fermentation liquid, the mycelium is dried, pulverized, sieved, degreased and dried, then extracted with hot water and concentrated to obtain a concentrated extraction solution. ; after ultrafiltration to remove small molecular substances, the fermentation broth is concentrated to obtain an ultrafiltration concentrate. The ultrafiltration concentrate and the extraction concentrate are deproteinized, ethanol precipitated, hydrogen peroxide decolorized, dialyzed and finally vacuum freeze-dried to obtain the intracellular polysaccharide extract of Yunzhi and the extracellular polysaccharide extract of Yunzhi respectively. The preparation process of the invention is simple, can be mass-produced with high efficiency and high quality, the extract is non-toxic to human body, has good physiological effect of lowering blood fat, and can be used for developing blood fat lowering medicine or health food.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polysaccharide extract of versicolor versicolor and its preparation method and application. Background technique [0002] Fermentation products are obtained by liquid submerged fermentation of edible and medicinal fungal mycelia, and the intracellular polysaccharides in the mycelium and the exopolysaccharides in the fermentation broth can generally be extracted separately. [0003] Hu Chengxu et al. (2007) used response surface methodology to study the water extraction process of polysaccharides from Yunzhi mycelia, and obtained the optimal water extraction conditions: extraction temperature 85°C, extraction time 105min, liquid-solid ratio 27:1, Yunzhi The extraction rate of polysaccharide was 7.27%. Pan et al. (2010) studied the optimal process parameters of polysaccharides from versicolor versicolor fruiting bodies by ultrasonic-assisted extraction, and obtained the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P3/06A61P9/10A23L33/125C12R1/645A23L33/00
Inventor 张松张命龙
Owner SOUTH CHINA NORMAL UNIVERSITY
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