Method for cultivating auricularia polytricha through thorn scraps
A technology of agaric and vitex shavings is applied in the field of edible fungus cultivation, can solve problems such as waste of wild vitex, and achieve the effects of realizing reuse, broad application prospect and low price.
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[0037] Embodiment 1, the method utilizing Vitex shavings to cultivate Auricularia auricula, the Auricularia auricula strain is purchased in the market
[0038] A. Preparation of liquid strains
[0039] First-class liquid culture medium formula: 20% potato, 3% bran, 2% glucose, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC 0.3%, natural pH;
[0040] Secondary liquid culture medium formula: Vitex powder 5%, potato 20%, bran 3%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC 0.3%, natural pH; among them, Vitex twigs are crushed and sieved through an 80-mesh sieve to obtain a powder, boiled with potatoes and bran for 30 minutes, filtered to remove slag to obtain an extract.
[0041] Use a 500mL Erlenmeyer flask with a liquid volume of 200mL for the preparation of the first-level liquid strains. After autoclaving at 121°C for 30 minutes, insert commercially available PDA slant strains under aseptic conditions, and the inoculation volume of each bottle is 4 piec...
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