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Detoxification and fermentation method of large red algae biomass degradation liquid

A fermentation method and technology for degrading liquid, applied in fermentation, biofuel and other directions, can solve the problems of single type of inhibitor, increased extra cost, low removal efficiency, etc., and achieve the effects of low cost, short time consumption and fast start-up speed.

Inactive Publication Date: 2015-03-18
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of inhibitors that can be removed by these methods are single, and the removal efficiency is low
In addition, there are also related studies to reduce inhibitors in the fermentation broth by optimizing the pretreatment process, or improving strains to enhance tolerance to inhibitors, but these methods will greatly increase additional costs and are still in the research and development stage

Method used

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  • Detoxification and fermentation method of large red algae biomass degradation liquid
  • Detoxification and fermentation method of large red algae biomass degradation liquid
  • Detoxification and fermentation method of large red algae biomass degradation liquid

Examples

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Embodiment 1

[0025] In this embodiment, the algae biomass is Gelidiales, and the liquid acid hydrolyzate of the algae biomass is anaerobically fermented to obtain ethanol. The detoxification agents are activated carbon and bentonite. The specific process is as follows.

[0026] (1) Take 200 grams of dry rock cauliflower, crush it, add 1 liter of 2% sulfuric acid solution, and mix well. Under the environment of 125 degrees centigrade, acid hydrolyze for 2 hours, and press filter to obtain the degradation liquid of Geliflower. Sodium hydroxide was added to the degradation solution to adjust the pH to 6.0.

[0027] (2) Measure 50 ml of degradation solution, add 1 g of activated carbon, and detoxify in a shaker for 30 minutes at a speed of 150 rpm. After detoxification, remove the activated carbon by suction filtration. Then, take 1 gram of calcium-based bentonite, add it into the detoxified degradation liquid through activated carbon, and carry out double detoxification. bentonite, and th...

Embodiment 2

[0041] In this embodiment, the algae biomass is Grateloupia, and the enzymatic hydrolysis product of the algae biomass is subjected to anaerobic fermentation to obtain ethanol. The detoxification agent is β-molecular sieve and diatomaceous earth.

[0042] (1) Weigh 300 grams of centipede algae powder, add 1 liter of 4% phosphoric acid aqueous solution and mix well. Degrade at 100°C for 1 hour to make up for the evaporated water. Use sodium hydroxide powder to adjust the pH of the acid degradation solution to 6.5, add 200 U / g of liquefying enzyme, and degrade at 60°C for 72 hours. After the liquefaction enzyme degradation process was completed, the pH of the degradation solution was adjusted to 5.0 with hydrochloric acid, and then 100 U / g of glucoamylase was added to continue the enzymatic hydrolysis at 60°C for 72 hours, and then squeezed and filtered to obtain the degradation solution.

[0043] (2) Measure 50 ml of the degradation solution, adjust the pH to 6.0 with sodium ...

Embodiment 3

[0057] In this embodiment, the algae biomass is Porphyra, and the acid gas explosion hydrolyzate of the algae biomass is anaerobically fermented to obtain butanol. The detoxification agent is active carbon, ZSM-5 molecular sieve and NaY molecular sieve.

[0058] (1) Weigh 300 grams of laver powder, add 0.5 liter of 4% nitric acid aqueous solution, mix well, and soak at room temperature for 2 hours. Put it into a steam explosion machine, process it at 180 degrees Celsius and 1.2Mpa for 3 minutes, quickly release the pressure, add 0.5 liters of water to the obtained product, stir well, squeeze and filter to obtain a degradation solution, and use calcium hydroxide powder to adjust the pH of the degradation solution to 7.0.

[0059] (2) Measure 50 ml of degradation solution, add 0.25 g of activated carbon, stir continuously at 50 degrees Celsius for 10 minutes for detoxification, and remove the activated carbon by suction filtration after detoxification. Then add 0.25 g of ZSM-5...

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Abstract

The invention provides a detoxification and fermentation method of large red algae biomass degradation liquid. The detoxification and fermentation method comprises the following steps: degrading the large red algae biomass to obtain the degradation liquid, adding an appropriate amount of molecular-sieve or non-molecular-sieve adsorbent having adsorption property as a detoxifying agent into the degradation liquid to carry out double detoxification or multiple detoxification on the degradation liquid so as to remove substances of preventing the activities of microorganisms in the degradation liquid, finally removing the detoxifying agent and producing bio-fuels or chemical intermediates by fermentation of the corresponding fermentation microorganisms. By virtue of experimental verification, compared with the existing fermentation method of algae, the detoxification and fermentation method disclosed by the invention has the advantages that the treatment process of the degradation liquid is simple and consumes less time; the detoxifying agent is low in cost, wide in resources and can be recycled; the fermentation of the microorganisms has the advantages of high starting speed, short time, high yield and less byproducts and the fermented products are easily separated and purified.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a new method for detoxifying and fermenting macroalgae biomass degradation liquid. Background technique [0002] With the development of human industrialized society, the environmental pollution and non-renewable problems faced by fossil fuel energy are becoming more and more serious. Biomass energy, as a renewable energy, has the characteristics of environmental friendliness and large quantity, and is expected to gradually replace fossil energy and become an emerging energy substance. At present, most of the production of biomass energy uses terrestrial plants as raw materials, especially food crops such as corn and sugarcane are widely used due to their high carbohydrate content. However, the cultivation of terrestrial plants occupies the land and fresh water resources necessary for human survival, and long-term development will inevitably affect human life. [...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P7/16
CPCC12P7/04C12P7/06C12P7/16C12P7/18Y02E50/10
Inventor 杨池张亚杰马中森吕学兰陆贻超
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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