Preparation method of paste welan gum
A technology of body temperature wheel and solid material, applied in fermentation and other directions, can solve problems such as affecting the use efficiency, cumbersome use process, incomplete dissolution, etc., and achieve the effects of environmental protection loss, convenient application and easy source of raw materials
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Embodiment 1
[0057] The preparation method of ointment Wenlun glue comprises inoculating sphingomonas (Sphingomonas sp) CGMCCNo.1561 in a sterile fermentation medium containing carbon source, nitrogen source, inorganic salt and water for fermentation; also includes the following steps :
[0058] A. Extraction
[0059] Extracting from the fermented liquid after the fermentation is finished, the extractant is selected from one of ethanol or ethanol solution, so that the ethanol concentration in the mixed liquid is 75%;
[0060] B. Solid-liquid separation
[0061] Perform solid-liquid separation on the mixed material to obtain solid material;
[0062] C. Preparation of ointment body temperature wheel glue
[0063] The solid material is added with water to make a paste through a colloid mill.
[0064] The extraction time in the described step A is 40 minutes.
[0065] After the fermentation, the fermented liquid was heated up to 90°C and maintained for 10 minutes, and then extracted after...
Embodiment 2
[0086] The preparation method of ointment Wenlun glue comprises inoculating sphingomonas (Sphingomonas sp) CGMCCNo.1561 in the aseptic fermentation medium containing carbon source, nitrogen source, inorganic salt and water and fermenting; also includes the following steps :
[0087] A. Extraction
[0088] Extracting from the fermented liquid after fermentation, the extractant is selected from one of ethanol or ethanol solution, so that the ethanol concentration in the mixed liquid is 70%;
[0089] B. Solid-liquid separation
[0090] Perform solid-liquid separation on the mixed material to obtain solid material;
[0091] C. Preparation of ointment body temperature wheel glue
[0092] The solid material is added with water to make a paste through a colloid mill.
[0093] The extraction time in the described step A is 30 minutes.
[0094] After the fermentation, the fermented liquid was heated up to 80° C. and maintained for 5 minutes, and then extracted after cooling down t...
Embodiment 3
[0115] The preparation method of ointment Wenlun glue comprises inoculating sphingomonas (Sphingomonas sp) CGMCCNo.1561 in the aseptic fermentation medium containing carbon source, nitrogen source, inorganic salt and water and fermenting; also includes the following steps :
[0116] A. Extraction
[0117] Extracting from the fermented liquid after the fermentation is finished, the extractant is selected from one of ethanol or ethanol solution, so that the ethanol concentration in the mixed liquid is 72%;
[0118] B. Solid-liquid separation
[0119] Perform solid-liquid separation on the mixed material to obtain solid material;
[0120] C. Preparation of ointment body temperature wheel glue
[0121] The solid material is added with water to make a paste through a colloid mill.
[0122] The extraction time in the described step A is 35 minutes.
[0123] After the fermentation, the fermented liquid was heated up to 85° C. and maintained for 8 minutes, and then extracted afte...
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