Method for separating S-ademetionine from yeast fermentation liquor

Abstract

The invention relates to a method for preparing S-ademetionine (SAM), in particular to a method for separating S-ademetionine from a yeast fermentation liquor, and belongs to the field of functional foods and preparation methods thereof. The method for separating S-ademetionine from the yeast fermentation liquor comprises the following steps: filtering the yeast fermentation liquor, performing centrifugation on the filtrate, pretreating collected substrate yeast, performing cell disruption treatment according to an extruded cell disruption method, carrying out centrifugation to obtain a supernatant, adding an acetone solvent, carrying out filtering, collecting a precipitate, and freeze-drying at low pressure to obtain the S-ademetionine. According to the preparation method provided by the invention, the disruption rate of yeast cells can reach about 85%, and the extraction rate of the S-ademetionine can reach more than 75%; the preparation method has the characteristics of being simple in process, high in operability, suitable for industrial production and high in recovery rate.

Description

technical field [0001] The invention relates to a method for preparing adenosylmethionine, which belongs to the field of functional food and its preparation method. Background technique [0002] Adenosylmethionine (SAM), also known as adenosylmethionine, is the most important methyl donor in the human body. This substance can inhibit the proliferation of retroviruses (such as HIV) in vitro, and is useful for the prevention and treatment of many diseases and maintaining human health are vital. In 1999, the US FDA approved SAM as a health product, and quickly became one of the best-selling nutritional products in the United States. In recent years, foreign countries have turned to the treatment of liver diseases, and the market demand continues to increase, which has a huge market prospect. [0003] SAM can be obtained from microbial fermentation extraction, chemical synthesis or enzymatic synthesis. Chemical synthesis has obvious disadvantages such as low yield and racemiz...

AI Technical Summary

Problems solved by technology

Chemical synthesis has obvious disadvantages such as low yield and racemization, so it is rarely used in practice; enzymatic synthesis, although the reaction product has high purity, is too expensive, and is generally only used to synthesize isotope-labeled SAM; some microorganisms can pass through cells. Metabolism, if there is a certain amount of methionine in the medium, it can convert and accumulate a higher concentration of SAM in the cell