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Raoultella terrigena for synthesizing 2,5-furan dicarboxylic acid and application of raoultella terrigena

A technology of furandicarboxylic acid and bacteria, which is applied in the field of microorganisms, and achieves the effect of simple method and good application prospect

Active Publication Date: 2015-02-25
西宝生物科技(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of Raoultella terrestris to produce 2,5-furandicarboxylic acid has not been reported

Method used

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  • Raoultella terrigena for synthesizing 2,5-furan dicarboxylic acid and application of raoultella terrigena
  • Raoultella terrigena for synthesizing 2,5-furan dicarboxylic acid and application of raoultella terrigena
  • Raoultella terrigena for synthesizing 2,5-furan dicarboxylic acid and application of raoultella terrigena

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 The screening of high-yield 2,5-furandicarboxylic acid strains

[0042] Add 1 g of a soil sample taken from a nearby factory producing 5-hydroxymethylfurfural into a 250 mL Erlenmeyer flask containing 20 mL of enrichment medium, and incubate at 30°C and 220 rpm for 24 hours. After the culture solution was centrifuged for a short time, the supernatant was taken and applied to the selection medium for cultivation for 48 hours. Pick the strains with larger colonies and transfer them to the seed medium for 24 hours of cultivation, then transfer 500 μL of the culture solution into a preservation tube containing 500 μL of 40% glycerol, and store in a -80°C refrigerator.

[0043] Enrichment medium or fermentation medium (g / L): tryptone 10g, yeast powder 5g, sodium chloride 10g, 5-hydroxymethylfurfural 3.15g.

[0044] Screening medium (g / L): tryptone 10g, yeast powder 5g, sodium chloride 10g, 5-hydroxymethylfurfural 4.4g, agar 20g;

[0045] Pick the strains stored a...

Embodiment 2

[0049] Example 2 Molecular biological identification of 2,5-furandicarboxylic acid strain

[0050] The 16sDNA sequences were amplified from the genomes of the screened strains using bacterial universal primers (27F / 1492R), sequenced, and compared. The 16sRNA sequence of the strain is shown in SEQ ID NO.1. The sequence was compared in NCBI by Blast, and it was found that the similarity between the target sequence of the bacterial strain and Raoulleia terrigena (R. terrigena NBRC14941) was 98%. Known as Klebsiella terrigena (Klebsiella terrigena)). The strain was deposited in the China Center for Type Culture Collection on August 26, 2014, with the preservation number CCTCC No: M2014391, and the preservation address is Wuhan University, Wuhan, China.

Embodiment 3

[0051] Morphological characteristics and physiological and biochemical characteristics of embodiment 3Raoultella terrigena BF60

[0052] R. terrigena BF60 was cultured at 30°C for 24 hours on a screening medium without 5-hydroxymethylfurfural, the colony was light yellow hemispherical, the surface was wet and shiny, the edges were neat, translucent, facultative anaerobic growth, Gram Staining was negative, 1000 × microscope (attached figure 1 ) and transmission electron microscopy (attached figure 2 ) observed under the rod-shaped cells, with capsules, single or in pairs. Corresponding physiological and biochemical performance analysis experiments were carried out with reference to the eighth edition of "Berger's Bacterial Identification Handbook" and related literature, and the results are shown in Table 1.

[0053] Table 1 Classification and identification results of target strains

[0054]

[0055] This result is consistent with the characteristics shown by Raoultell...

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Abstract

The invention discloses raoultella terrigena for synthesizing 2,5-furan dicarboxylic acid and an application of the raoultella terrigena, and belongs to the technical field of microorganisms. The raoultella terrigena BF60 strain disclosed by the invention is preserved at the China Center for Type Culture Collection on August 26, 2014; and the preservation number is CCTCC No.M2014391. The raoultella terrigena is screened from the soil near the factory of producing 5-hydroxymethyl furfural. The yield of 2,5-furan dicarboxylic acid which is produced by whole-cell transformation on the raoultella terrigena can reach 7.95g / L; and a foundation is laid for further utilization of native raoultella terrigena to produce 2,5-furan dicarboxylic acid. The method for producing 2,5-furan dicarboxylic acid by using the raoultella terrigena disclosed by the invention is simple, and has a good application prospect.

Description

technical field [0001] The invention relates to a Raoultella terrestris for synthesizing 2,5-furandicarboxylic acid and its application, belonging to the technical field of microorganisms. Background technique [0002] 2,5-Furandicarboxylic acid (2,5-Furandicarboxylic acid, FDCA) is an important chemical raw material and organic synthesis intermediate, which can be used to prepare various lipid furan or alkyl-substituted derivatives, and is listed by the U.S. Department of Energy as One of the 12 most promising platform compounds. In addition, 2,5-furandicarboxylic acid can replace terephthalic acid to produce polyester plastic materials, which has broad application prospects. [0003] At present, the methods for preparing 2,5-furandicarboxylic acid include chemical synthesis and biotransformation. The chemical synthesis generally uses noble metals as catalysts, which requires high temperature, high pressure and organic solvents. The cost is high and the environment is seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/22
CPCC12P17/04C12N1/205C12R2001/01
Inventor 刘龙陈坚堵国成李江华袁海波
Owner 西宝生物科技(上海)股份有限公司
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