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Collagen matrix cartilage repairing membrane, and making method and application thereof

A collagen matrix, cartilage repair technology, applied in medical science, prosthesis and other directions, can solve problems such as cartilage calcification, and achieve the effects of inhibiting cartilage calcification, smooth surface and simple preparation process

Active Publication Date: 2015-02-25
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, some researchers have found signs of cartilage calcification during cartilage repair

Method used

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  • Collagen matrix cartilage repairing membrane, and making method and application thereof
  • Collagen matrix cartilage repairing membrane, and making method and application thereof
  • Collagen matrix cartilage repairing membrane, and making method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A) Strip the tendon from the leg of a two-month-old healthy white pig, remove the fascia and fat, cut into pieces 120g, soak in 500mL of 75% ethanol aqueous solution at 4 ℃ overnight; B) take 95g of the tendon fragments from step A Rinse 5 times with distilled water, add 0.5mol / L acetic acid aqueous solution 1800mL, adjust pH to 1 with 6M sodium hydroxide aqueous solution, add pepsin (Sigma-ALDRICH) 9g, stand at 4 ℃ for 3 days, and obtain 1800mL collagen sol solution; C) Centrifuge the collagen sol solution obtained in step B horizontally at 4°C and 4000rpm for 25 minutes, discard the upper fat layer, dilute the precipitate a with 0.5mol / L acetic acid aqueous solution to 3 times the volume, and filter it with 4 layers of gauze in an ultra-clean bench. Mix the filtrate with 0.9mol / L NaCl aqueous solution at a volume ratio of 1:10, shake intermittently, overnight at 4°C, then centrifuge horizontally at 4°C and 4000rpm for 25 minutes, discard the supernatant and suspension ...

Embodiment 2

[0030]The water-soluble type I collagen extracted by the method in Example 1 was dissolved in deionized water to prepare 10 mL of a collagen aqueous solution with a concentration of 10 mg / mL, and 40 mL of a 1M sodium bicarbonate solution was added for neutralization. Take out 4.3mL of the neutralized type Ⅰ collagen solution and pour it into a 6cm diameter cell culture dish (Corning). Place the culture dish in an oven with a temperature of 37°C and dry it for 48 hours to form a type I collagen film in the mold. Take out the dried type Ⅰ collagen film and cut it into 5mm×5mm size. Take 10 slices of cut membranes, place them in a cell culture dish (Corning) with a diameter of 10 cm, and add 5 mL of parathyroid hormone-related peptide (Bachem) solution with a concentration of 100 ug / mL prepared in PBS (pH=7.4) , placed in a cell incubator and soaked at 37°C for 1 hour to obtain a type Ⅰ collagen membrane material compounded with parathyroid hormone-related peptide, that is, a co...

Embodiment 3

[0032] The water-soluble type I collagen extracted by the method in Example 1 was dissolved in deionized water to prepare 10 mL of a collagen aqueous solution with a concentration of 5 mg / mL, and 10 mL of a 1M sodium bicarbonate solution was added for neutralization. Take out 4.3mL of the neutralized type Ⅰ collagen solution and pour it into a 6cm diameter cell culture dish (Corning). Place the culture dish in an oven with a temperature of 37°C and dry it for 48 hours to form a type I collagen film in the mold. Take out the dried type Ⅰ collagen film and cut it into 5mm×5mm size. Take 10 slices of cut membranes, place them in a cell culture dish (Corning) with a diameter of 10 cm, and add 5 mL of parathyroid hormone-related peptide (Bachem) solution with a concentration of 100 ug / mL prepared in PBS (pH=7.4) , placed in a cell incubator at 37°C for 1 hour to obtain a type I collagen membrane material compounded with parathyroid hormone-related peptide, that is, a collagen matr...

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Abstract

The invention discloses a making method of a collagen matrix cartilage repairing membrane. The method comprises the following steps: 1, shearing a raw material pork leg tendon, carrying out enzymatic hydrolysis, centrifuging, filtering, and dissolving to obtain an I type collagen solution; 2, neutralizing the I type collagen solution obtained in step 1 by using a 1M sodium bicarbonate solution; 3, pouring the neutralized I type collagen solution obtained in step 2 into a die, and drying at 30-40DEG C for 35-54h to form an I type collagen matrix membrane in the die; and 4, immersing the I type collagen matrix membrane obtained in step 3 in a 20-200[mu]g / mL parathyroid hormone related peptide solution, and incubating at 30-40DEG C for 0.5-2h to obtain the parathyroid hormone related peptide compounded I type collagen matrix cartilage repairing membrane. The invention also discloses the collagen matrix cartilage repairing membrane and an application thereof. The cartilage repairing membrane has certain adhesiveness to injured surfaces of cartilage, and is suitable for repairing cartilage injuries.

Description

technical field [0001] The invention relates to the field of biomaterial preparation, in particular to a rapid preparation method and application of a collagen matrix cartilage repair membrane containing active factors. Background technique [0002] Articular cartilage can reduce the friction between two adjacent bones and buffer the vibration generated during exercise, so cartilage damage caused by sports is extremely common. Statistics show that there are more than 400,000 cartilage-related surgeries in the United States alone every year. Because cartilage has no blood vessels and nerves, the traditional view is that cartilage damage cannot be repaired spontaneously. If no external intervention is taken, it will lead to further cartilage wear and even osteoarthritis, causing irreversible damage to its structure and function. Patient's quality of life work. The current conservative treatment methods for clinical treatment of superficial cartilage injuries include joint ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/54
Inventor 张薇谢淑君陈隆坤
Owner ZHEJIANG XINGYUE BIOTECH
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