Helicobacter pylori serotype classification method and helicobacter pylori biochip construction method

A technology of Helicobacter pylori and typing method, which is applied in the direction of determination/inspection of microorganisms, microorganisms, and biological material analysis, etc., can solve the problem of not giving a guidance plan, etc., and achieves strong repeatability, high sensitivity, and high accuracy. Effect

Inactive Publication Date: 2015-02-18
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV +2
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  • Claims
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Problems solved by technology

[0009] The above technologies do not provide specific guidance on how to provide

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  • Helicobacter pylori serotype classification method and helicobacter pylori biochip construction method
  • Helicobacter pylori serotype classification method and helicobacter pylori biochip construction method
  • Helicobacter pylori serotype classification method and helicobacter pylori biochip construction method

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Embodiment 1

[0029] Embodiment 1: the Helicobacter pylori serotyping method of the present invention is to establish the Helicobacter pylori serotyping method according to the virulence gene and the Helicobacter pylori structure of a certain area (a certain administrative prefecture-level city area) , the virulence gene of described Helicobacter pylori refers to cagA and vacA, and described Helicobacter pylori structure refers to flagella and lipopolysaccharide repeat oligosaccharide and Lewis blood group structure, and described serotyping method comprises the following steps:

[0030] (1) Isolate Helicobacter pylori from patients with Helicobacter pylori, see Method 1 for the isolation culture (and identification) method.

[0031] Method 1. Isolation, cultivation and identification of gastric Helicobacter pylori: the gastric mucosal tissue of patients infected with Helicobacter pylori was bitten by gastroscope, and then the gastric mucosal tissue was ground into a slurry in a glass grinde...

Embodiment 2

[0071] Embodiment 2: The construction method of the Helicobacter pylori biochip of the present invention is to extract the lipopolysaccharide and flagella of the virulent epidemic strain of Helicobacter pylori in a certain area to prepare the biochip. The virulent epidemic strain of Helicobacter pylori is based on the Helicobacter virulence genes (cagA and vacA), Helicobacter pylori flagella and lipopolysaccharide structure screened out virulence epidemic strains, the biochip includes a solid phase carrier and immobilized on the carrier according to the serotype named strain Lipopolysaccharide and flagellin probes, labeled secondary antibodies against human IgG and IgM, and a result interpretation system, the construction method specifically includes the following steps: (1) isolate Helicobacter pylori from Helicobacter pylori patients, isolate and culture (And identification) method sees the method one of embodiment 1. (2) PCR and sequencing of cagA and vacA were performed on...

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Abstract

The invention discloses a helicobacter pylori serotype classification method and a helicobacter pylori biochip construction method. According to the methods, helicobacter pylori is extracted from a patient with helicobacter pylori; the extracted helicobacter pylori has PCR (polymerase chain reaction) of cagA and vacA and is sequenced; after sequencing for helicobacter pylori virulence genes cagA and vacA is finished, a regional virulence epidemic strain is screened out based on gene combination and is named; lipopolysaccharide and flagellum of the helicobacter pylori virulence epidemic strain are extracted and subjected to structural analysis, and serotype naming is performed according to virulence genes, the structure of the flagellum, the Lewis blood type structure of lipopolysaccharide and repeated oligosaccharide structure; and lipopolysaccharide and flagellum of the named strain are extracted to construct the biochip. The helicobacter pylori serotype classification method and the helicobacter pylori biochip construction method can greatly simplify detection means, has the characteristics of high accuracy, high throughput and high repeatability, and can meet requirements of clinical diagnosis, specific treatment and epidemiological investigation for helicobacter pylori.

Description

technical field [0001] The invention relates to a serotyping method for bacteria, in particular to a Helicobacter pylori serotyping method, and also relates to a construction method for a Helicobacter pylori biochip. The invention belongs to the field of biotechnology. Background technique [0002] In 1983, Warren and Marshall successfully isolated Helicobacter pylori (Hp) from gastric mucosal biopsies in patients with chronic gastritis. After further research, it was found that Hp infection is closely related to the occurrence of diseases such as chronic gastritis, peptic ulcer, gastric cancer, and gastric MALT lymphoma. Class I carcinogens. With extensive and in-depth research on Hp, some scholars believe that Hp infection is also related to the pathogenesis of allergic purpura, coronary heart disease, diabetic complications, Parkinson's disease and Alzheimer's disease. Therefore, the diagnosis and active treatment of Helicobacter pylori can effectively reduce the incid...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C40B50/06C12R1/01
CPCC12Q1/689C12Q1/04G01N33/56911G01N2333/20G01N2400/50
Inventor 单保恩赵川史中立殷长甫王苋胡代伦
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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