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A rapid construction method for methanogenesis under acidic conditions

A construction method and acidic condition technology, which is applied in the field of rapid construction of methanogens in acidic conditions and stable treatment of high-concentration organic matter, can solve the problems of spatial inhomogeneity and inability to determine whether the methanogens are acid-resistant, and achieve shortened debugging and obvious Economic value and development potential, the effect of increasing daily processing capacity

Active Publication Date: 2017-08-15
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the artificial environment of landfills, although there are signs of methane production in landfills that have been acidified for a long time, it takes 0.5 to 1 year, and due to the spatial inhomogeneity of waste dumps, it is impossible to determine the methane production. Whether bacteria are acid-resistant (references: Staley, B.F.; de los Reyes, F.L., III; Barlaz, M.A., Effect of spatial differences in microbial activity, pH and substrate levels on methanogenesis initiation in refuse. Applied and Environmental Microbiology 2011, 77, ( 7), 2381-2391)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Take 10kg of anaerobic digestion treatment dehydration residue (solid content 20%, VS content 0.7) in the middle temperature 35 ℃ of urban sewage plant sludge, grind, and use 50 mesh screens to sieve, collect its sieve as Seed mud.

[0029] (2) Put 8.6kg of the above-mentioned seed sludge in the 250L anaerobic reactor (equivalent to 1.2kg of VS total amount), 1.2kg of acetic acid is used as a methane-producing substrate, and add base buffer solution until the total volume of the solution in the reactor is 200L. When the concentration of acetate is 100mM. The main composition of the base buffer is (1L): 6g Na 2 CO 3 , 0.2g MgCl 2 ·6H 2 O, 1g NH 4 Cl, 0.1g CaCl 2 , 0.2gNa 2 S·9H 2 O, 1.385g K 2 HPO 4 , 1.4g KH 2 PO 4 , 5mg Na 2 -EDTA·2H 2 O, 1.5mg CoCl 4 , 1mg MnCl 2 4H 2 O, 1mg FeSO 4 ·7H 2 O, 1mg ZnCl 2 , 0.4mg AlCl 3 ·6H 2 O, 0.3mg NaWo 4 2H 2 O, 0.2mg CuCl 2 2H 2 O, 0.2mg NiSO 4 ·6H 2 O, 0.1 mg H 2 SeO 4 , 1mg HBO 3 , 1mgNaMoO 4 2H ...

Embodiment 2

[0036] (1) Take 10kg of municipal solid waste dehydration residue from high-temperature 55°C anaerobic digestion treatment (solid content 30%, VS content 0.7), grind it, and sieve it with a 70-mesh sieve, and collect the under-sieve material as seed mud .

[0037] (2) Put 8.6kg of the above-mentioned seed sludge in the 250L anaerobic reactor (equivalent to 1.8kg of VS total amount), 2.16kg of acetic acid is used as the methane-producing substrate, and the base buffer solution is added until the total volume of the solution in the reactor is 200L. When the acetate concentration was 120mM. The main composition of the base buffer is (1L): 8g Na 2 CO 3 , 0.2g MgCl 2 ·6H 2 O, 1g NH 4 Cl, 0.1gCaCl 2 , 0.2g Na 2 S·9H 2 O, 1.385g K 2 HPO 4 , 1.4g KH 2 PO 4 , 5mg Na 2 -EDTA·2H 2 O, 1.5mg CoCl 4 , 1mg MnCl 2 4H 2 O, 1mg FeSO 4 ·7H 2 O, 1mg ZnCl 2 , 0.4mg AlCl 3 ·6H 2 O, 0.3mg NaWo 4 2H 2 O, 0.2mg CuCl 2 2H 2 O, 0.2mg NiSO 4 ·6H 2 O, 0.1 mg H 2 SeO 4 , 1mg H...

Embodiment 3

[0044] (1) Take 10kg of anaerobic granular sludge (solid content 20%, VS content 0.7) in the high temperature 60 ℃ anaerobic digestion reactor of the wastewater treatment device of the paper mill, grind it, and use a 50-mesh screen to sieve points, and collect the undersieve as seed mud.

[0045] (2) Put 8.6kg of the above-mentioned seed sludge in the 250L anaerobic reactor (equivalent to 1.2kg of VS total amount), 1.2kg of acetic acid is used as a methane-producing substrate, and add base buffer solution until the total volume of the solution in the reactor is 200L. When the concentration of acetate is 100mM. The main composition of the base buffer is (1L): 6g Na 2 CO 3 , 0.2g MgCl 2 ·6H 2 O, 1g NH 4 Cl, 0.1g CaCl 2 , 0.2gNa 2 S·9H 2 O, 1.385g K 2 HPO 4 , 1.4g KH 2 PO 4 , 5mg Na 2 -EDTA·2H 2 O, 1.5mg CoCl 4 , 1mg MnCl 2 4H 2 O, 1mg FeSO 4 ·7H 2 O, 1mg ZnCl 2 , 0.4mg AlCl 3 ·6H 2 O, 0.3mg NaWo 4 2H 2 O, 0.2mg CuCl 2 2H2 O, 0.2mg NiSO 4 ·6H 2 O, 0.1 m...

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Abstract

The invention relates to a quick construction method for generating methane under an acidic condition. The quick construction method comprises the following steps: pretreating seed sludge, performing habituated culture on the seed sludge to form acid-resisting acidophilus microorganism agglomerate rich in acid-resisting acidophilus methanogens and other symbiotic bacteria, inoculating the acid-resisting acidophilus microorganism agglomerate to a closed reactor containing fresh or acidulated perishable organic waste, and conducting anaerobic digestion to generate methane. Compared to the prior art, the methane generating method disclosed by the invention can maintain the methane generating activity under the acidic condition (at the pH value of 5.5-6.2), implement high organic loading anaerobic treatment to generate the methane with the organic waste at a lower inoculation rate, and further can be used for quickly enabling a rancidified anaerobic digester to restore generation of methane.

Description

technical field [0001] The invention belongs to the fields of environmental protection and comprehensive utilization of resources, and relates to a process for anaerobic digestion of waste to produce methane, in particular to a method for quickly constructing and stably treating high-concentration organic matter in acidic conditions for methane production. Background technique [0002] Solid waste such as kitchen waste, food waste, food processing waste, and fermentation industrial waste is huge, and because of its high proportion of easily degradable organic components, such as starch, protein, fat, etc., it is easy to corrupt, also known as Perishable organic waste. Taking Shanghai as an example, the daily collection and transportation of kitchen waste has reached 13,000 tons / day, and the collection and transportation of food waste has reached 2,797 tons / day. If such a huge amount of perishable organic waste is not properly disposed of, it will cause serious secondary pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02
CPCY02E50/30
Inventor 吕凡何品晶邵立明蔡涛刘玉浩章骅
Owner TONGJI UNIV
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