A method for separating and purifying glycolipids
A technology for separation and purification of glycolipids, applied in the field of deep processing of soybean oil waste, can solve the problems of low separation efficiency, difficulty in satisfying further research on glycolipid structure identification and biological activity, and long time consumption, and achieve the highest yield of glycolipids high effect
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Embodiment 1
[0029] Embodiment 1: Separation and purification of glycolipids from crude phospholipids
[0030] Use a measuring cylinder to take 500ml (567g) of crude phospholipids (soybean oil leftovers) into a 2000ml large beaker, add 750ml of n-hexane, and keep stirring until the crude phospholipids are completely dissolved, then transfer them to a 2000ml separating funnel. Prepare 750ml of 50% (w / w) ethanol aqueous solution, pour 250ml of ethanol aqueous solution into the n-hexane phospholipid system for the first time, shake up and down for 5 minutes, then let stand to separate layers, take the upper ethanol aqueous solution; pour 250ml of ethanol for the second time Put the aqueous solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for stratification, and take the upper layer of ethanol water solution; for the third time, pour 250ml of ethanol water solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, the...
Embodiment 2
[0041] Embodiment 2: Separation and purification of glycolipids from crude phospholipids
[0042] Use a measuring cylinder to take 500ml (550g) of crude phospholipids into a 2000ml large beaker, add 750ml of n-hexane, and keep stirring until the crude phospholipids are completely dissolved, then transfer to a 2000ml separatory funnel. Prepare 750ml of 60% (w / w) ethanol aqueous solution, pour 250ml of ethanol aqueous solution into the n-hexane phospholipid system for the first time, shake up and down for 5min, then let stand to separate layers, take the upper layer of ethanol aqueous solution; pour 250ml of ethanol for the second time Put the aqueous solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for stratification, and take the upper layer of ethanol water solution; for the third time, pour 250ml of ethanol water solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for strati...
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