Cordyceps sinensis liquid fermentation process

Abstract

The invention provides a cordyceps sinensis liquid-state fermentation process, which comprises the following steps of (1) inclined surface culture: preserved cordyceps sinensis strains are inoculated onto an inclined surface culture medium to be cultured at 25-DEG C constant temperature for five days; (2) seed liquid culture: the activated strains are inoculated into a seed culture medium to be cultured for three days in a table concentrator with the rotating speed being 140r / min and the temperature being 24 DEG C; (3) fermentation culture: a fermentation culture medium is added into a 500mL conical flask, the liquid containing quantity is 100mL, liquid seeds are inoculated according to the inoculating quantity being 30-percent v / v, the initial pH of the fermentation liquid is 6.51, and the seeds are cultured for four days under the conditions of the temperature being 24 DEG C and the table concentrator rotating speed being 140r / min, wherein the consumption of glucose is 40.84g / L, and the consumption of peptone is 21.55g / L. The yield of mycelia, the intercellular polysaccharide content and the adenosine content in the cordyceps sinensis obtained by the process provided by the invention are respectively and obviously improved, and great application values are realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a liquid-state fermentation process of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a compound of the fungal subsea (called "grass") developed by Cordyceps sinensis parasitic on the larvae of the bat moth of Cordyceps sinensis and the dead larva full of mycelium (called "worm"). In taxonomy, Cordyceps sinensis Belongs to the fungi, Ascomycota, Sclerotinia, Hypocreales, Ergotaceae, Cordyceps. The chemical composition of Cordyceps sinensis is complex, containing active ingredients such as cordycepin, nucleosides, amino acids, polypeptides, polysaccharides, sugar alcohols, sterols, fatty acids, esters, alkanes, polyamines, etc., and has various pharmacological functions. Such as anti-tumor, anti-oxidative aging, anti-myocardial ischemia and arrhythmia, regulation of endocrine, regulation of immunity, regulation of lipid metabolism, sedative center...

AI Technical Summary

Problems solved by technology

However, the cultivation period of Cordyceps sinensis using solid is long, labor-intensive, and easily affected by external factors. There is an urgent need for an efficient, stable, and high-quality artificial cultivation process.

The existing liquid fermentation process of Cordyceps sinensis often only uses a single mycelium yield as an index, and does not significantly improve the intracellular polysaccharide and adenosine content of Cordyceps sinensis.