PCR (polymerase chain reaction) reaction solution and kit, and PCR method

Abstract

The invention relates to the technical field of biology, particularly a PCR (polymerase chain reaction) reaction solution and kit, and a PCR method. The PCR reaction solution is prepared from dimethyl sulfoxide (DMSO), bovine serum albumin (BSA), dNTP (deoxyribonucleotide triphosphate), 10*PCR buffer, bromphenol blue solution, glycerol solution with the solute volume percent of 30%, and rTaq enzyme. The PCR reaction solution for PCR experiments is optimized; especially a certain proportion of glycerol solution is added, and the combination of the BSA and DMSO is added to ensure the stability and activity of the Tag enzyme, reduce the formation of the primer dimer and shorten the PCR reaction solution configuration time; and in the PCR product detection process by agarose gel electrophoresis, the detection can be performed only by adding the nucleic acid dye, thereby saving abundant time for detecting the PCR product and enhancing the PCR amplification efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a PCR reaction solution, a kit and a PCR method. Background technique [0002] Polymerase chain reaction (PCR) is a technique for rapidly amplifying specific DNA fragments in vitro, and it is one of the most important means of modern molecular biology. Its basic principle is based on the mechanism of somatic cell replication established by the DNA double helix model won the Nobel Prize in Physiology in 1953 and the characteristic that double-stranded DNA can undergo denaturation and renaturation in vitro with temperature changes. In 1985, Randll.K.Skaii established the polymerase chain reaction technology using Klenow DNA polymerase and applied it to the genetic diagnosis of sickle cell anemia. It was a revolutionary breakthrough in the field of molecular biology in the 1980s, and was hailed as another milestone in the development history of molecular biology resources. Once this t...

AI Technical Summary

Problems solved by technology

[0005] In conventional PCR amplification system configuration, DNTP, Buffer, rTaq enzyme and other reagents are often stored separately. When PCR amplification is to be performed, the samples are added to the same PCR tube separately. In addition to often forgetting to add individual reagents during the operation, In addition to the disadvantages, repeated use will cause pollution to the reagents and affect subsequent use

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