Application of globeflower flavonoids in preparation of medicine for treating itch dermatoses
A technology of pruritic skin disease and nasturtium, applied in the field of medicine, to achieve the effect of broadening the scope of application, increasing market value, and controlling quality
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Embodiment 1
[0012] Embodiment 1, preparation and content determination of total flavonoids of Trollius chinensis
[0013] The preparation method of the total flavonoids of Trollius chinensis, the specific steps are as follows: Weigh 2 kg of Trollius chinensis medicinal material, add water equivalent to 10 times the weight of the medicinal material and decoct twice for 1 hour each time, filter the decoction and combine the filtrate, centrifuge , take the supernatant and go over the macroporous adsorption resin D101 column (weak polarity, Tianjin Haiguang Chemical Co., Ltd.), then wash it with 0.2% hydrochloric acid solution until the effluent is colorless, then wash it with distilled water until the effluent The solution was neutral, and then eluted with ethanol solution with a volume fraction of 50%. The ethanol eluate was collected, concentrated under reduced pressure, and dried to obtain total flavonoids of Trollica chinensis. Utilize this method to obtain the amount of total flavonoids...
Embodiment 2
[0020] Embodiment 2, the pharmacological effect of total flavonoids of Trollius chinensis
[0021] 1. The emulsifiable paste made from total flavonoids of Trollius vulgaris in Example 1 detects the influence of total flavonoids of Trollius vulgaris on chronic eczema model
[0022] Get 50 Kunming male mice whose body weight is about 20g, and divide them into 5 groups at random, 10 in each group, respectively the control group, the model group, the Ai Luo Song group, the high total flavonoids of Trollius chinensis (drug content 2%, w / w), medium (drug content 1%, w / w), low dose group (drug content 0.5%, w / w). On the day before the experiment, the hair was removed on the abdomen, with an area of about 3×3cm 2 . Except for the control group, the rest of the groups were evenly coated with 1% 2,4-dinitrofluorobenzene (DNFB) on the abdominal hair removal area, 100 μL / body was sensitized, and the next day was strengthened once, and the first sensitization On the 7th day, 12th day...
Embodiment 3
[0055] Embodiment 3, the acute toxicity of total flavonoids of Trollius chinensis
[0056] Get 40 mice with a body weight of 20 ± 2g, half male and half male, each mouse is given total flavonoids of Trollica chinensis (equivalent to crude drug 20g / kg) at a dose of 1.0g / kg (body weight), and administered by oral gavage every day 3 times, continuous administration for 7 days, observe whether the mice have acute toxicity.
[0057] The results showed that when the total flavonoids of nasturtium lotus was orally administered to mice (10 mg / kg in mice), which was equivalent to 100 times the dose of human medicine, no mice died and LD was not detected. 50 . The above results show that the total flavonoids of Trollius chinensis has good safety, which is equivalent to 100 times the dosage of human medicine, and no death of mice has been seen.
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