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Trichoderma chlamydospore wettable powder composition and preparing method thereof

A technology of wettable powder and chlamydospores, which is applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of uninspected germination, improve field control efficiency and reduce the use of , the effect of extending the shelf life

Active Publication Date: 2015-02-04
HEBEI BAIAO BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN101874484A discloses a paste formulation of Trichoderma biopesticide, which is a preparation containing Trichoderma mycelia and Trichoderma chlamydospores prepared with starch as a carrier. The spores still have high activity, but the main indicators such as their germination ability have not been investigated

Method used

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  • Trichoderma chlamydospore wettable powder composition and preparing method thereof
  • Trichoderma chlamydospore wettable powder composition and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Trichoderma chlamydospore powder 20 parts by weight

[0019] Sodium lauryl sulfate 4 parts by weight

[0020] Carboxymethylcellulose 5 parts by weight

[0021] Dextrin 1 part by weight

[0022] 70 parts by weight of diatomaceous earth

[0023] The preparation steps of this embodiment are:

[0024] Set the concentration to 1 x 10 8 The cfu / ml Trichoderma conidia solution was inoculated into the sterilized Chlamydospore culture medium at an inoculation amount of 5%, and cultured on a shaker at 28°C and 180rpm / min for 7 days.

[0025] Stir with a stirrer at 3000 r / min for 1 min to break up the mycelia mass to separate the chlamydospores from the mycelium, then centrifuge at 3000 r / min for 10 min to finally obtain the chlamydospore slurry.

[0026] After mixing the chlamydospore slurry and diatomaceous earth at a ratio of 7:1, drying and pulverizing at 50°C, the chlamydospore powder is obtained.

[0027] Weigh 20 parts by weight of Trichoderma chlamydospore powder, 4 ...

Embodiment 2

[0029] Trichoderma chlamydospore powder 15 parts by weight

[0030] 7 parts by weight of sodium dodecylbenzenesulfonate

[0031] 3 parts by weight of sodium methylenebisturide sulfonate

[0032] 2 parts by weight of methylcellulose

[0033] 73 parts by weight of diatomaceous earth

[0034] The preparation steps of this embodiment are:

[0035] Set the concentration to 1 x 10 8 The cfu / ml Trichoderma conidia solution was inoculated into the sterilized Chlamydospore culture medium at an inoculation amount of 10%, and cultured on a shaker at 28°C and 180rpm / min for 7 days.

[0036] Stir with a stirrer at 3000 r / min for 1 min to break up the mycelia mass to separate the chlamydospores from the mycelium, then centrifuge at 3000 r / min for 10 min to finally obtain the chlamydospore slurry.

[0037] After mixing the chlamydospore slurry and diatomaceous earth at a ratio of 7:1, drying and pulverizing at 50°C, the chlamydospore powder is obtained.

[0038] Weigh 15 parts by weigh...

Embodiment 3~8

[0040] The preparation method of Examples 3-8 is the same as that of Example 1, the difference is that different weight parts or different types of additives are selected, and all the examples use diatomaceous earth to make up 100 weight parts.

[0041] Table 1 Composition of Trichoderma chlamydospore wettable powder

[0042]

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Abstract

The invention relates to a trichoderma chlamydospore wettable powder composition and a preparing method thereof. The trichoderma chlamydospore wettable powder composition includes 15 to 20 parts by weight of a trichoderma chlamydospore powder, 3 to 7 parts by weight of a wetting agent, 3 to 10 parts by weight of a dispersing agent, 0 to 3 parts by weight of a UV protection agent, and other component which is a carrier and is added to 100 parts by weight. The composition and a trichoderma chlamydospores preparation prepared by the method are used as a biological pesticide; biggest advantages comprise that the preparation is the biological pesticide, does not pollute the environment, can reduce the use of chemical pesticides, and is conducive to environmental protection; compared with a conidiospore preparation, the preparation can prolong the shelf life of the biological pesticide, is saved for 6 months at the normal temperature, and has the spore germination rate of more than 80% which is higher than 60% of the conidiospore preparation; and compared with the conidiospore preparation, the preparation can improve the field prevention effect.

Description

technical field [0001] The invention relates to a trichoderma chlamydospore wettable powder composition. Background technique [0002] Trichoderma is a biocontrol fungus with important economic value. Since scientists first discovered that Trichoderma lignin can parasitize a variety of plant pathogenic fungi in 1932, after years of research, it has been found that Trichoderma is antagonistic to 29 pathogenic fungi of 18 genera. More than 50 kinds of Trichoderma preparations have been successfully developed, among which Topshield (T. harzianum T22) in the United States and Trichodex (T. harziana T39) in Israel are the most widely used. Most of the commercial Trichoderma preparations are conidia preparations or mixed preparations of conidia and mycelia. Due to the characteristics of the conidia itself, the Trichoderma conidia preparations have a short shelf life and poor control stability in the field. Chlamydospore is a propagule produced by Trichoderma under adversity condi...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/14A01P3/00
Inventor 黄亚丽张晶晶马宏刘昆昂贾振华赵芊黄媛媛张立文宋聪宋水山李书生史延茂郝少彦
Owner HEBEI BAIAO BIOLOGICAL PROD
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