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A kind of highly stable lactic acid bacteria microcapsules and its preparation method and application

A lactic acid bacteria microcapsule and high stability technology, applied in the field of bioengineering, can solve the problems of weakening microcapsule strength, affecting inoculation efficiency, increasing production cost, etc., to prevent phage infection, improve inoculation efficiency, and reduce unit cost Effect

Active Publication Date: 2018-10-30
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is aimed at the current microencapsulated starter in the continuous inoculation process, as the use time increases, the strength of the microcapsules weakens and the breakage rate increases, which seriously affects the inoculation efficiency, reduces production capacity, and increases production costs. Provide a lactic acid bacteria-containing microcapsule with high mechanical strength, good stability during continuous culture and continuous inoculation, and long service life, which improves inoculation efficiency and reduces the unit cost of fermented products

Method used

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  • A kind of highly stable lactic acid bacteria microcapsules and its preparation method and application

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Effect test

Embodiment 1

[0040] A preparation method for highly stable lactic acid bacteria microcapsules, comprising the steps of:

[0041] (1) Inoculate the freeze-dried lactic acid bacteria into the MRS medium to activate the bacteria twice.

[0042] (2) Inoculate the strain activated in step (1) into whey medium or MRS medium, and culture at 37° C. for 18 hours.

[0043] (3) The fermented liquid obtained in step (2) is centrifuged (5000rpm, 10min) and discarded to remove the supernatant, and the remaining bacteria are suspended in sterile trehalose aqueous solution, mixed with calcium lactate, sodium caseinate and gelatin-free Bacteria mixed aqueous solution is mixed evenly; In the described trehalose aqueous solution, the solute trehalose mass percent concentration is 0.5%; The mass ratio of the bacteria body to the sterile trehalose aqueous solution is 35%; In the sterile mixed aqueous solution of gelatin, the mass percentage concentration of solute calcium lactate is 4%, the mass percentage co...

Embodiment 2

[0056] A preparation method for highly stable lactic acid bacteria microcapsules, comprising the steps of:

[0057] (1) Inoculate the freeze-dried lactic acid bacteria into the MRS medium to activate the bacteria twice.

[0058] (2) Inoculate the strain activated in step (1) into whey medium or MRS medium, and culture at 30° C. for 20 hours.

[0059] (3) The fermented liquid obtained in step (2) is centrifuged (5000rpm, 10min) and discarded to remove the supernatant, and the remaining bacteria are suspended in sterile trehalose aqueous solution, mixed with calcium lactate, sodium caseinate and gelatin-free Bacteria mixed aqueous solution is mixed; in the described trehalose aqueous solution, the solute trehalose mass percent concentration is 0.6% (preferably 0.5%); the mass ratio of the bacteria to the sterile trehalose aqueous solution is 32%; the calcium lactate, In the sterile mixed aqueous solution of sodium caseinate and gelatin, the mass percentage concentration of solu...

Embodiment 3

[0066] A preparation method for highly stable lactic acid bacteria microcapsules, comprising the steps of:

[0067] (1) Inoculate the freeze-dried lactic acid bacteria into the MRS medium to activate the bacteria twice.

[0068] (2) Inoculate the strain activated in step (1) into whey medium or MRS medium, and culture at 44° C. for 24 hours.

[0069] (3) The fermented liquid obtained in step (2) is centrifuged (5000rpm, 10min) and discarded to remove the supernatant, and the remaining bacteria are suspended in sterile trehalose aqueous solution, mixed with calcium lactate, sodium caseinate and gelatin-free Bacteria mixed aqueous solution is mixed; In the described trehalose aqueous solution, the solute trehalose mass percent concentration is 0.8%; The mass ratio of the bacteria body to the sterile trehalose aqueous solution is 38%; The calcium lactate, sodium caseinate and In the sterile mixed aqueous solution of gelatin, the mass percentage concentration of solute calcium la...

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Abstract

The invention discloses a high-stability lactic acid bacteria microcapsule, a preparation method and application thereof, and belongs to the technical field of bioengineering. The lactic acid bacteria cell suspension is mixed with a sterile sodium caseinate, gelatin and calcium lactate mixture, passed through a microencapsulated cell aseptic preparation device, and dropped into a sterile sodium alginate solution to form a lactic acid bacteria liquid core capsule. Bacteria-containing capsules are treated with transglutaminase to cross-link sodium caseinate and gelatin in the capsule wall, which enhances the mechanical strength of the capsules, increases the stability of continuous culture and continuous inoculation, and prolongs the continuous inoculation of bacteria-containing capsules. Time, improve the service life of bacteria-containing capsules, reduce the unit cost of fermented products, improve the economic benefits and market competitiveness of enterprises, and have broad market prospects.

Description

technical field [0001] The invention relates to a high-stability lactic acid bacteria microcapsule and its preparation method and application, belonging to the technical field of bioengineering, and is a method for increasing the stability of continuous culture and continuous inoculation process, prolonging the continuous inoculation time of bacteria-containing capsules, and improving the lifespan of bacteria-containing capsules. Service life, a new technology to improve the efficiency of continuous inoculation starters such as fermented dairy products and fermented fruit and vegetable juices. Background technique [0002] Traditional liquid starter refers to the purebred culture used for production, which is used for production after step-by-step expansion and multiplication of mother starter, intermediate starter, and production starter. When this type of starter is finally used, the bacterial concentration is only 10 8 -10 9 cfu / ml, and it is complicated in operation an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10A23C11/10A23C9/127A23L2/02A23L11/50A23L11/65
Inventor 周剑忠夏秀东王英刘小莉李莹张丽霞黄自苏
Owner JIANGSU ACAD OF AGRI SCI
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