Pharmaceutical composition having functions of defying age and enhancing physique, preparation method and detection method thereof
A technology for enhancing physique and composition, which is applied in the directions of drug combination, pharmaceutical formula, medical preparation containing active ingredients, etc., can solve the problems of reducing the overall curative effect, disrupting the strict rules of compatibility of traditional Chinese medicines, and the undisclosed dosage of Lijiashou tablets, etc. achieve good curative effect
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Embodiment 1
[0069] Embodiment 1: the preparation method of film-coated tablet:
[0070] Step 1: Take ginseng stems and leaves or reed heads, grind them, add ethanol with a volume ratio of 80% and soak for 3 hours, the amount of ethanol is 10 times the amount of ginseng (weight ratio), ultrasonic extraction 4 times, ultrasonic power 1000W, each extraction 30 Minutes, filtered; the filtrates were combined, concentrated under reduced pressure until there was no alcohol smell, diluted the concentrated solution with water to 3 times, mixed evenly, and stood at low temperature for 24 hours, filtered to obtain an extract, the filtrate was concentrated under reduced pressure, and applied to D101 macroporous resin, Rinse with deionized water, discard, then rinse with 1% ammonia water by volume, discard, then rinse with deionized water until the pH of the effluent is 7, elute with 30% ethanol solution by volume, and collect the eluate , concentrated under reduced pressure, and dried to obtain total...
experiment example 1
[0088] Research on the effects of anti-aging and physical fitness-enhancing pharmaceutical compositions on aging-related indicators in aged rats:
[0089] The pharmaceutical composition for anti-aging and physical fitness is composed of total ginsenosides extracted by the method of the present invention, Ganoderma lucidum dry extract, Astragalus extract, Epimedium extract, Radix Paeoniae Alba extract, Acanthopanax extract and vitamin E etc. 7 Flavor medicine composition. In this experiment, old rats were taken as the research objects, and the anti-aging effect of the pharmaceutical composition for anti-aging and physical fitness enhancement was observed with aging-related indicators as the standard.
[0090] 1. Materials
[0091] 1.1 Reagents
[0092] Superoxide dismutase (SOD) activity assay kit: Nanjing Jiancheng Institute of Bioengineering, batch number: 20130502.
[0093] Malondialdehyde (MDA) activity assay kit: Nanjing Jiancheng Institute of Bioengineering, batch numb...
experiment example 2
[0140] Research on the influence of anti-aging and physical fitness-enhancing pharmaceutical composition on exhaustive swimming test in mice:
[0141] 1 material
[0142] 1.1 Test animals
[0143] 40 Kunming mice were provided by Shanghai Slack Experimental Animal Co., Ltd., license number: SCXK (Shanghai) 2013-0007, weighing (22±2) g, half male and half male.
[0144] 1.2 Instruments
[0145] Centrifuge: LD5-2A type, produced by Beijing Medical Centrifuge Factory, GC-1200r radioimmunity counter.
[0146] 2. Method
[0147] 2.1 Grouping and administration
[0148] Anti-aging and physical fitness-enhancing pharmaceutical composition: be formulated into three groups of different pharmaceutical compositions of A, B, and C by Table 1, and 3 groups of different pharmaceutical compositions are added distilled water to be mixed into 0.12g (dry extract and vitamin E composition) / ml solution. The daily gavage amount of 0.23g dry extract and vitamin E composition / kg body weight (e...
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