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A kind of isochlorathion colloidal gold rapid detection test strip and preparation method thereof

A technology of isochlorothion and detection test paper, which is applied in measurement devices, instruments, scientific instruments and other directions, can solve the problems of application limitation of ELISA method, difficult to meet the rapidity, online detection, and the detection results are not intuitive enough, and achieve the results display image, It is easy to promote and determine the effect of the image

Inactive Publication Date: 2016-09-07
JURONG AGRI TECH PROMOTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods include high performance liquid chromatography (High performance liquid chromatography, HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), liquid-mass tandem method (HPLC-MS) and gas chromatography. - mass tandem method (GC-MS), etc. Although these instrumental analysis methods can achieve high detection sensitivity, they require complex and expensive instruments and professional operators. , the need for online testing
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA method is greatly limited.

Method used

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  • A kind of isochlorathion colloidal gold rapid detection test strip and preparation method thereof
  • A kind of isochlorathion colloidal gold rapid detection test strip and preparation method thereof
  • A kind of isochlorathion colloidal gold rapid detection test strip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A kind of isochlorathion colloidal gold rapid detection test strip, described test strip contains liner 1 and the sample pad 2 that is arranged on the liner in sequence, gold label binding pad 3, cellulose membrane 4 and water-absorbing pad 7, The liner 1 is a tough material that does not absorb water. The tough material is made of hard plastic strips. The gold-labeled binding pad 3 is adsorbed with isochlorathion gold-labeled antibody. There are invisible detection line 5 and invisible control line 6, the invisible detection line 5 is printed with carrier protein solution coupled with isochlorathion hapten, and the invisible control line 6 is printed with rabbit anti-mouse IgG antibody. The water-absorbing pad 7 is water-absorbing filter paper or oil filter paper with strong water-absorbing capacity. Isochlorthion gold-labeled antibody is colloidal gold-labeled isochlorthion monoclonal antibody. The carrier protein for coupling isochlorathion hapten is bovine serum al...

Embodiment 2

[0038] It is basically the same as Example 1, except that the tough material is made of non-absorbent hard paper strips, and the water-absorbing pad 7 is oil filter paper with strong water-absorbing capacity. The carrier protein for coupling isochlorthion hapten is chicken ovalbumin OVA. The sample pad 2 is made of nylon film, polyvinylidene fluoride film and polyester film. Cellulose membranes are pure cellulose membranes.

Embodiment 3

[0040]It is basically the same as in Example 1, except that the tough material is made of hard plastic strips, and the water-absorbing pad 7 is water-absorbing filter paper with strong water-absorbing capacity. The carrier protein coupled with the isochlorathion hapten is human serum albumin HAS. The sample pad 2 is made of polyvinylidene fluoride film and polyester film. The cellulose film is a carboxylated cellulose film.

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Abstract

The invention discloses a rapid test strip for isochlorothion colloidal gold and a preparation method of the rapid test strip. The rapid test strip comprises a lining plate as well as a sample pad, a gold mark binding pad, a cellulose membrane and a water absorption pad which are sequentially arranged on the lining plate, wherein isochlorothion gold mark antibodies are adsorbed in the gold mark binding pad; a stealth detection line and a stealth contrast line are arranged on the cellulose membrane, the stealth detection line is printed by an isochlorothion hapten-coupled carrier protein solution, and the stealth contrast line is printed by rabbit antimouse IgG antibodies. The rapid test strip has the advantages of being strong in specificity, high in sensibility, simple, convenient, rapid, and capable of visually and accurately displaying results.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue immune analysis, and in particular relates to a rapid detection test strip of isochlorathion colloidal gold and a preparation method thereof. Background technique [0002] Isochlorathion is a white crystalline solid, insoluble in water, soluble in acetone, cyclohexane, toluene, xylene, ethyl acetate, used as an agricultural insecticide, this substance is harmful to the environment, special attention should be paid to the pollution of water bodies . It is recommended to dispose of it by controlled incineration or safe landfill. The oral LD50 of male rats is 400 and 460 mg / kg, the oral value of female rats is 330 mg / kg, and the transdermal values ​​of male and female are 790 and 1 respectively. Hydrolysis Conversely, there is a substantial portion of hydrolysis of alkyl phosphates and other dialkyl compounds. [0003] At present, the detection methods for pesticide residues are mainly i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/559G01N33/577
CPCG01N33/531G01N33/54306G01N33/558
Inventor 韩志成
Owner JURONG AGRI TECH PROMOTION CENT
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