A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation
A liquid fertilizer and seaweed technology, which is applied in the field of seaweed liquid fertilizer preparation by enzymatic fermentation, can solve the problems of refractory macromolecular substances of seaweed, affecting the effect of seaweed fertilizer, and soil without any beneficial effect, so as to promote plant cell division, Effect of increasing vascular bundle cells and improving internal voids
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Embodiment 1
[0019] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:
[0020] (1) Kelp pretreatment: rinse the kelp with clean water, remove impurities, and drain; cut the kelp into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 Soak the kelp in the solution for 3 times, each time for 15 minutes, and perform desalination and color protection treatment; dry at 85°C, finally grind it, and pass through a 40-mesh sieve;
[0021] (2) Ultrasonic enzymatic hydrolysis: add water 8 times the mass of kelp, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharide enzyme, the addition amount is 3% and 2% of the mass of kelp, ultrasonic at 40-60°C Auxiliary enzymatic hydrolysis for 2 hours, ultrasonic power 400w, frequency 25kHz;
[0022] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, CaCl 2 , the addition amount...
Embodiment 2
[0027] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:
[0028] (1) Enteromorpha pretreatment: rinse Enteromorpha with water, remove impurities, drain; cut Enteromorpha into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 The solution soaked Enteromorpha 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally ground into powder, and passed through a 40-mesh sieve;
[0029] (2) Ultrasonic enzymatic hydrolysis: add water 9 times the mass of Enteromorpha, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharide enzyme, the addition amount is 2% and 1% of the mass of Enteromorpha, at 40-60°C Ultrasonic assisted enzymolysis for 4 hours, ultrasonic power 400w, frequency 25kHz;
[0030] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, ...
Embodiment 3
[0035] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:
[0036] (1) Sargassum pretreatment: rinse Sargassum with clean water, remove impurities, and drain; cut Sargassum into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 The solution soaked the Sargassum 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally pulverized, and passed through a 40-mesh sieve;
[0037] (2) Ultrasonic enzymatic hydrolysis: add water 10 times the mass of Sargassum, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharase, the addition amount is 2.5% and 1.5% of the mass of Sargasso, at 40-60°C Ultrasonic-assisted enzymatic hydrolysis for 3 hours, ultrasonic power 400w, frequency 25kHz;
[0038] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, CaCl ...
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