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Electronic nose technology based method for rapid identification of pilose antler ''authenticity''

An electronic nose and deer antler technology, which is used in material analysis, measuring devices, instruments and other directions by electromagnetic means, can solve the problems of long detection time, serious degradation of sample DNA, complicated and complicated process, etc. The effect of fast speed and high work efficiency

Inactive Publication Date: 2014-12-31
王学勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process is cumbersome and complicated, and the detection time is long, so the purpose of rapid identification cannot be achieved.
With the gradual rise of molecular identification, the identification of velvet medicinal materials has also been further developed, and great achievements have been made in identifying the authenticity of velvet antler and solving the chaos in the deer medicinal material market. However, with the deepening and promotion of DNA barcoding technology research , People find that it is often limited in practical work, such as the sample DNA degradation is more serious due to the influence of processing and other factors, resulting in DNA barcoding technology has defects such as long experimental period, stability, and low operability

Method used

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  • Electronic nose technology based method for rapid identification of pilose antler ''authenticity''
  • Electronic nose technology based method for rapid identification of pilose antler ''authenticity''
  • Electronic nose technology based method for rapid identification of pilose antler ''authenticity''

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Comparative Analysis of velvet Genuine Products and Counterfeit Products Based on First Vector Feature Extraction

[0018] Each antler sample is injected and tested separately, as follows:

[0019] Treatment of experimental materials: take velvet antler into a petri dish, and wrap each one with newspaper. Put it in an oven at 40°C for 12 hours. On the one hand, it is dry and easy to crush, and on the other hand, it is easy to volatilize the alcohol residue in the process of cutting the velvet slices.

[0020] The settings of the electronic nose are as follows: injection volume 2500 μL, incubation time 500 s, incubation temperature 70 °C, acquisition time 200 s, delay time 400 s, stirring speed 250 rpm.

[0021] Six replicates were performed for each sample.

[0022] Use the maximum response value of each sensor for each sample in the sample set to build a library, and perform PCA and LDA (Figure-1) analysis. In the PCA analysis experiment, the acquired data...

Embodiment 2

[0023] Example 2 Comparative Analysis of Genuine Deer Antler and Counterfeit Products Based on Second Vector Feature Extraction

[0024] Each antler sample is injected and tested separately, as follows:

[0025] Treatment of experimental materials: take velvet antler into a petri dish, and wrap each one with newspaper. Put it in an oven at 40°C for 12 hours. On the one hand, it is dry and easy to crush, and on the other hand, it is easy to volatilize the alcohol residue in the process of cutting the velvet slices.

[0026] The settings of the electronic nose are as follows: injection volume 2500 μL, incubation time 500 s, incubation temperature 70 °C, acquisition time 200 s, delay time 400 s, stirring speed 250 rpm.

[0027] Six replicates were performed for each sample.

[0028] Use the maximum response value of each sensor for each sample in the sample set to build a library, and perform PCA and LDA (Figure-2) analysis. In the PCA analysis experiment, the acquired data wa...

Embodiment 3

[0029] Example 3 Electronic nose antler identification analysis second vector feature model verification

[0030]Extract the second vector feature data in the verification set, combine it with the second vector feature data in the sample set to build a database, and perform LDA analysis. A two-dimensional column chart (Figure-3) was established for the prediction results of each antler slice. From the figure, it can be concluded that the correct classification rates of sika deer, red deer and reindeer in the initial grouping cases were 97.82%, 84.0%, and 97.77%. The correct classification rate is 94.8%. In addition, this model also verified the difference between genuine sika deer and other kinds of velvet antler such as moose, sambar, Eld's deer, elk, etc., and the correct identification rate reached 83.23%-98.36%. It has better discrimination and recognition ability, and is suitable for popularization and application of deer antler identification.

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Abstract

The invention relates to an electronic nose technology based method for rapid identification of the ''authenticity'' of Chinese medicine pilose antler. The method comprises the steps of: crushing a pilose antler sample and putting the crushed sample into a sealed container, under certain temperature condition, sucking gas into an electronic nose sensor array channel, letting sensors respond to obtain certain response signal; subjecting the response signal to feature information extraction, comparing multiple mathematical algorithms, establishing an algorithm suitable for pilose antler identification, thus finishing correlation analysis of the response signal and pilose antler indexes, and realizing effective and rapid identification of commercially available Cervus elaphus Linnaeus, reindeers, Cervus nippon Temminck and other different types of pilose antlers. The method established by the invention can rapidly identify the ''authenticity'' of pilose antler medicinal materials, decoction tablets, pilose antler powder and other different forms of raw materials. Compared with existing identification methods, the method has the technical advantages of objectivity, rapidity, sensitiveness, simplicity, no need of tedious pretreatment and the like, and has broad application prospects in identification of animal drugs, and precious Chinese medicinal materials, etc.

Description

technical field [0001] The invention relates to a method for quickly identifying "authenticity" of traditional Chinese medicine velvet antler based on electronic nose technology. Background technique [0002] "Pharmacopoeia of the People's Republic of China" (2010 Edition) stipulates that velvet antler is the unossified and densely hairy young horns of the male deer Cervus nippon Temminck or red deer Cervus elaphus Linnaeus, which is a tonic for nourishing essence, strengthening kidneys and tendons. Deer antler is a valuable Chinese medicinal material with great market demand. There are many counterfeit products in the market, and the varieties are chaotic. Most of the counterfeit products on the market are reindeer. For a long time, the identification of the authenticity of deer antler has mainly relied on the traditional trait identification method for species classification and identification. This method is relatively subjective, empirical, and random, and it is difficul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00
Inventor 王学勇廖采丽黄璐琦吴浩忠刘春生杨瑶珺
Owner 王学勇
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