Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of vaccine composition and its preparation method and application

A vaccine composition and immunization technology are applied to the vaccine composition of Streptococcus suis and its preparation, the vaccine composition and its preparation, the field of swine fever virus, and can solve the problems of economic loss, swine fever vaccine immunization failure and the like

Active Publication Date: 2018-02-02
PU LIKE BIO ENG
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to immunosuppression, the immunization of swine fever vaccine will fail, causing serious economic losses.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Preparation of Antigens of Swine Fever and Streptococcus Suis

[0051] 1 Preparation of swine fever antigen

[0052] 1.1 Digest and disperse the highly sensitive ST cells (purchased from ATCC) that have grown into a good monolayer and are highly sensitive to the attenuated rabbit strain of CSFV with a digestive solution containing 0.125% trypsin and 0.03% EDTA, and inoculate the cells for cell culture after counting the cells bottle, add 3% calf serum MEM cell culture solution, and at the same time add seed poison according to M.O.I.=0.1 inoculation dose, and place it in a 37°C incubator for cultivation.

[0053] After cultivating for three days, the poison was collected for the first time, and the cell maintenance solution containing 1.5% calf serum was added after the poison collection, and the poison was collected once every 2 days thereafter, and the poison could be collected 5 times continuously. After harvesting the virus, the antigens were mixed and stored at -2...

Embodiment 2

[0086] Preparation of swine fever and Streptococcus suis vaccine composition

[0087] 1 Combined use of inactivated vaccine diluted with live vaccine

[0088] 1.1 Get the hog fever whole virus antigen prepared in Example 1, add heat-resistant protective agent (2wt% gelatin aqueous solution and 15wt% lactose aqueous solution are prepared in 1:1 (v / v) ratio) with 1:1 (v / v) ratio After mixing, fully mix, quantitatively dispense, and freeze and vacuum-dry quickly, that is, live swine fever vaccine.

[0089] 1.2 Take the whole bacterial antigen of Streptococcus suis prepared in Example 1, slowly add it to the water-soluble adjuvant gel adjuvant (Seppic, France), and continuously stir it with an emulsifier at 800 rpm for 12 minutes during the addition process, and mix well , stored at 4°C, it is the inactivated vaccine of Streptococcus suis.

[0090] 1.3 When in use, dilute the live swine fever vaccine with inactivated Streptococcus suis vaccine, and the specific formulation of th...

Embodiment 3

[0099] Immunogenicity test of swine fever and Streptococcus suis vaccine composition

[0100] 1 Immunization program for piglets

[0101] 170 piglets that were 25-30 days old and negative for swine fever and Streptococcus suis antigen antibodies were divided into 17 groups for the test. The 1st-2nd group was the vaccine 1 immunization group, the 3rd-4th group was the vaccine 2 immunization group, and the 5th- Group 6 is vaccine 3 immunization group, Group 7-8 is vaccine 4 immunization group, Group 9-10 is vaccine 5 immunization group, Group 11-12 is vaccine 6 immunization group, and Group 13-14 is vaccine 7 immunization group group, the 15th-17th group is the PBS control group. Each pig in the PBS control group was injected with 1ml of PBS with a ratio of 10% (V / V) gel adjuvant, and each pig in the vaccine immunized group was injected with 1ml of the vaccine in the neck muscle for a single immunization.

[0102] 2 Challenge protection test of immune piglets

[0103] 21 days...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of veterinary biological products. More specifically, the present invention relates to a vaccine composition of classical swine fever virus and Streptococcus suis and a preparation method thereof, said composition comprising antigens of classical swine fever virus and Streptococcus suis. The present invention also relates to the application of the vaccine composition for preparing a composition for preventing and / or treating diseases related to classical swine fever virus and Streptococcus suis and infection caused by classical swine fever virus and Streptococcus suis.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a vaccine composition and its preparation method and application. More specifically, the present invention relates to a vaccine composition of classical swine fever virus and Streptococcus suis and a preparation method thereof, the composition comprising antigens of classical swine fever virus and Streptococcus suis. The present invention also relates to the application of the vaccine composition for preparing a composition for preventing and / or treating diseases related to classical swine fever virus and Streptococcus suis and infection caused by classical swine fever virus and Streptococcus suis. Background technique [0002] Streptococcus suis is an important pathogen of pigs, causing many pathological diseases such as arthritis, endocarditis, meningitis, pneumonia and septicemia. It is also an important zoonotic pathogen for humans exposed to contaminated pigs o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/09A61K39/187A61P31/04A61P31/14
CPCA61K39/092A61K39/12A61K2039/521A61K2039/5254A61K2039/552A61K2039/70C12N2770/24334
Inventor 李向东田克恭张海洋张许科孙进忠
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com