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Application of Phantanthin or Phantasmal Extract in Anti-influenza Virus Drugs

An anti-influenza virus, aquakinin technology, applied in the field of medicine, can solve the problem of anti-influenza virus that has not yet been seen

Active Publication Date: 2017-11-28
SHENZHEN NEPTUNUS PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that the total flavonoids extract of P. chinensis has antibacterial, anti-inflammatory, analgesic, anti-herpes virus, anti-ulcer and other activities, but there is no report of anti-influenza virus (Huang Xinbi, Long Shengjing. Ban Hongshu Research progress on the chemical constituents and pharmacological effects of the plant Pseudomonas [J]. Chinese Herbal Medicine, 2004,35(9):1073-1076.)

Method used

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  • Application of Phantanthin or Phantasmal Extract in Anti-influenza Virus Drugs
  • Application of Phantanthin or Phantasmal Extract in Anti-influenza Virus Drugs
  • Application of Phantanthin or Phantasmal Extract in Anti-influenza Virus Drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] [Example 1] Preparation and analysis of the total flavonoids extract

[0040] Take 1Kg of branches and leaves of Pygnus chinensis, crush them, add 10L of 80% ethanol to heat and reflux for extraction twice, each extraction for 2 hours, combine the extracts, and concentrate under reduced pressure with a rotary evaporator until there is no alcohol smell (concentrate to v / w≈3~4) , centrifuge, get the supernatant, and load it on the polyamide chromatography column (column volume 1L) with purified water as the initial solvent after washing away the residual solvent with ethanol first, and the loading flow rate is 1 column volume per hour; then, First elute 3 column volumes with purified water, then 5 column volumes with 50% ethanol, the elution flow rate is 2 column volumes per hour, collect the 50% ethanol eluate, concentrate under reduced pressure, and dry to obtain water Total flavonoids extract 13.6g of wampum bark, taking lutein as reference substance (preparation metho...

Embodiment 2

[0041] [Example 2] Preparation and Content Analysis of Phantanthin

[0042] Get 10g of the total flavonoids extract of Pseudomonas japonica obtained in Example 1, add 50mL of ethanol to heat and dissolve, then slowly add 100mL of water, centrifuge, get the supernatant and load it on the reversed-phase silica gel chromatography column that has been balanced with 40% ; Elution with 40% ethanol, thin-layer chromatography inspection eluent, collect the fractions containing hydroxanthin, dry under reduced pressure to obtain 2.7g of hydroxanthin crude product, after dissolving the hydroxanthin crude product with absolute ethanol, Add water until the concentration of ethanol is about 10%, place it, and recrystallize it to obtain 2.3g of luteolin, and use high performance liquid chromatography (chromatographic column: using octadecylsilane bonded silica gel as filler; mobile phase: methanol- 0.2%HAc (43:57); flow velocity: 1ml / min; detection wavelength: 260nm) the content of aquatin i...

Embodiment 3

[0043] [Example 3] The in vitro anti-influenza virus effect of total flavonoids extract or aquacetrin

[0044] Virus strain: Influenza A virus (A / PR / 8 / 34H1N1), purchased from China Center for Type Microorganisms Collection.

[0045] Cell model: dog kidney cell line MDCK, purchased from Shanghai Boyan Biotechnology Co., Ltd.; culture conditions: DMEM+10% fetal bovine serum, 37°C, 5% CO 2 .

[0046] Cytotoxicity testing of drugs: using (Invitrogen) kit was used to detect the toxic effect of drugs on cells. Experimental principle: is a redox indicator that produces absorbance changes and fluorescent signals based on metabolic activity. It is easily soluble in water, and its oxidized form enters the cells, and after reduction by mitochondrial enzymes, it produces measurable fluorescence and color changes, which are used for quantitative analysis of cell viability and cell proliferation, and in vitro cytotoxicity studies. This assay is based on the ability of metabolically a...

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PUM

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Abstract

The invention discloses the application of a total flavonoid extract of A. chinensis or A. chinensis in preparing a medicine for treating and / or anti-influenza virus. The total flavonoids extract of S. chinensis is extracted from the stems, branches and / or leaves of S. chinensis, and the content of total flavonoids is 50% to 70% by weight; wherein, the total flavonoids are contained; the content of the total flavonoids Taking hydroxanthin as a reference substance, it is determined by ultraviolet spectrophotometry; the content of hydroxanthin is 5% to 10% by weight.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to the application of total flavonoids extracts from the yellow cortex or yellow cortex. Background technique [0002] Influenza virus belongs to the Orthomyxoviridae family (Orthomyxoviridae), has 8 fragments of the envelope, and is a single-stranded negative RNA virus encoding 11 proteins. Influenza virus is the pathogen that causes influenza and is divided into three types: A, B, and C. Among them, type A is the most threatening to humans. Human influenza viruses are mainly H1, H2 and H3 subtypes, wherein the H1N1 subtype has the highest fatality rate, so the present invention mainly takes the H1N1 type influenza virus as the research object for the antiviral effect of the total flavonoids extract of P. activity research. [0003] At present, influenza is still an acute upper respiratory infectious disease that cannot be effectively controlled. It has the characteristics of h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61K31/352A61P31/16A61K135/00A61K127/00
Inventor 严启新谭道鹏曾伟珍陈艳萍王宇冯汉林
Owner SHENZHEN NEPTUNUS PHARM CO LTD
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