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Embryo quality assessment based on blastomere cleavage and morphology

一种胚胎质量、卵裂球的技术，应用在体外受精的胚胎领域

Active Publication Date: 2016-11-16
UNISENSE FERTILITECH AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while early cleavage and other early criteria may be quality indicators for development into embryos, there is still a need for quality indicators for successful implantation and thus successful pregnancy

Method used

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  • Embryo quality assessment based on blastomere cleavage and morphology
  • Embryo quality assessment based on blastomere cleavage and morphology
  • Embryo quality assessment based on blastomere cleavage and morphology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0139] Example 1 - Retrospective Study

[0140] Materials and Methods

[0141] The research program was carried out at the Valencia Infertility Institute (Instituto Valenciano de Infertilidad, IVI), Valencia. Procedures and protocols were approved by the Institutional Review Board (IRB), which prescribes and approves the database analysis and clinical IVF procedures for studies performed at IVI. The program complies with the Spanish Law (14 / 2006) governing Assisted Reproductive Technologies. A total of 2903 oocytes were included in this study, from which 2120 embryos were generated during 285 IVF treatment cycles between September 2009 and September 2010. All embryos were obtained after fertilization by intracytoplasmic sperm injection (ICSI) and were part of a clinical standard (n=188) and egg donation program (n=97). Time-lapse images of all embryos were acquired, but only those embryos that had been transferred with a known implantation outcome (i.e., 0% implantation or ...

Embodiment 2

[0185] Example 2 - Data analysis based on known implant data

[0186] The analysis was based on known implantation data (KID) of 1598 embryos from 10 different clinics. KID embryos are embryos that have been transferred and have known implantation outcomes. In the case of multiple embryo transfers, only embryos with complete failure or complete success in implantation are used. All multiple transfers where there was implantation but fewer embryos were implanted than were transferred were discarded to be able to determine the implantation success of a particular embryo. Implantation success is assigned a value of 1 if the transferred embryo implants successfully, and a value of 0 if implantation fails. The number of embryos (N) used to calculate the expected value (probability of success) for the target and non-target groups was different for the different variables.

[0187] single variable

[0188] For a single continuous variable (eg t2), divide the data into quartiles a...

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PUM

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Abstract

The present invention relates to a method and system for selecting embryos for in vitro fertilization based on the observed timing and duration of cell cleavage and associated cell morphology, and more particularly to a method for determining embryo quality, the The method includes monitoring an embryo for a period of time, and determining one or more quality criteria for the embryo, wherein the one or more quality criteria are based on when the embryo develops from four blastomeres to eight blastomeres The degree of irregularity in the timing of cell division, and / or based on the determination of the time to cleavage into five blastomere embryos (t5) where t5 is between 48.7 hours and 55.6 hours, and / or based on two time intervals ratio, each of the two time intervals being determined as the duration of the time period between two morphological events in embryonic development from fertilization to eight blastomeres, and based on the one or more mass Standards determine embryo quality.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 201280035768.1 with an application date of May 31, 2012 and an invention title of "Embryo Quality Evaluation Based on Cleavage and Morphology of Blastomeres". technical field [0002] The present invention relates to a method and system for selecting embryos for in vitro fertilization based on the observed timing and duration of cell cleavage and associated cell morphology. Background technique [0003] Infertility affects more than 80 million people worldwide. It is estimated that 10% of all couples suffer from primary or secondary infertility (Vayena et al., 2001). In vitro fertilization (IVF) is an optional medical treatment that can give couples who would not otherwise be able to conceive a chance to conceive. It is a process in which egg cells (oocytes) are removed from a woman's ovary and fertilized with sperm in a laboratory. Embryos formed during this process are then placed in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073G06K9/00G06T7/00
CPCG06T7/0016G06T2207/10016G06T2207/10056G06T2207/20224G06T2207/30044G06V20/695G01N33/5005
Inventor 尼尔斯·B·拉姆辛凯伦·玛丽·希利格索马科斯·梅赛盖尔·埃斯克里瓦
Owner UNISENSE FERTILITECH AS
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