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Compound probiotic peptide as well as preparation method and application thereof

The technology of probiotics and probiotics, which is applied in the field of compound probiotic peptides and their preparation, can solve the problems of hindering the digestion, absorption and utilization of nutrients by animals, endangering the health and growth and development of animals, etc., and achieves good industrialization implementation prospects and costs. Inexpensive, palatability-enhancing effect

Active Publication Date: 2014-12-24
九源岳阳生物科技饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the presence of anti-nutritional substances such as protease inhibitors, lectins, soybean antigenic proteins, urease, phytic acid, soybean oligosaccharides in soybeans not only greatly hinders the digestion, absorption and utilization of nutrients by animals, but also seriously Harmful to the health and growth of animals

Method used

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  • Compound probiotic peptide as well as preparation method and application thereof
  • Compound probiotic peptide as well as preparation method and application thereof
  • Compound probiotic peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] From May 2013 to September 2013, water samples, sediment, and intestinal feces of prawns were collected from many places along the coast of Jiaozhou. The bottom mud and shrimp intestinal feces samples were diluted 10 times in sterile normal saline, fully ground, and the supernatant was taken as the sample solution; the water sample was directly used as the sample solution; the sample solution was inoculated in 2216E liquid medium for enrichment at 30°C After culturing for 48 hours, it was diluted and spread on a 2216E plate to isolate and purify the strains. A total of 26 pure strains were screened out.

[0035] The purified single strains were screened for antagonistic bacteria by plate spotting method. Using Vibrio parahaemolyticus as indicator bacteria, Vibrio parahaemolyticus was inoculated in 2216E liquid medium and cultured for 18 hours, and diluted to 10 5 -10 6 CFU / mL bacterial suspension, take 0.1mL to coat 2216E plate, set aside. Pick the strains to be scre...

Embodiment 2

[0058] figure 1 The composition and half-year stability of the complex prebiotic peptide-probiotic bacteria prepared by No. 1 soybean meal after biological treatment are shown. figure 2 The composition and half-year stability of the compound prebiotic peptide-probiotic bacteria amount prepared by No. 2 soybean meal after biological treatment were shown. Before and after low-temperature drying treatment, the final compound probiotic peptide product and the bacteria in the fermented wet material have a certain proportion of survival, among which the survival rate of spores in drying is higher than 89%, and after half a year of storage at room temperature, the living bacteria survive rate above 90%. In the fermented wet material of No. 1 compound probiotic peptide, the amount of Clostridium butyricum reached 1.08×10 6 CFU / g, after drying at low temperature, containing Clostridium butyricum 9.7×10 5 CFU / g, the drying survival rate reached 89.81%, and the bacterial count remain...

Embodiment 3

[0061] The SDS-PAGE qualitative determination result of antigenic protein is as follows image 3 As shown, the ELISA quantitative determination results are shown in Table 1. The band depths of different protein subunits of No. 1 and No. 2 soybean meal raw materials are slightly different, and the specific measured values ​​of glycinin, β-soybean conglycinin, and antigenic protein are also slightly different, but the composite of soybean meal raw materials and biological treatment The comparative trend among prebiotic peptide products is similar: after biological treatment, the macromolecular antigen protein of compound prebiotic peptide is almost completely degraded into small molecular protein (protein molecular weight lower than 14KDa), from No. 1 and No. 2 soybean meal raw materials As for the compound prebiotic peptide products, the removal rates of antigenic proteins determined by ELISA kits also reached 91.98% and 93.21%, respectively.

[0062] Table 2 shows the changes...

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Abstract

The invention discloses a compound probiotic peptide as well as a preparation method and an application thereof. The compound probiotic peptide is obtained by adding a bacterium enzyme compounding fermentation agent into soya bean meal and fermenting, wherein the bacterium enzyme compounding fermentation agent is formed by mixing four types of probiotics, four types of proteases and a carbon source; the four types of probiotics comprise clostridium butyricum, bacillus pumilus S3, lactobacillus plantarum and saccharomyces boulardii; and the four types of proteases comprise an acidic protease, a neutral protease, an alkaline protease and a polypeptide protease. The invention provides a specific preparation method of the compound probiotic peptide and an application of the compound probiotic peptide to preparing a piglet feed additive. The compound probiotic peptide product is enriched with a plurality of types of probiotics and antigen proteins can be effectively degraded to generate micro-molecular substances, namely polypeptides, amino acids and the like; and in a fermentation process, a plurality of types of flavor and antagonistic substances, namely probiotic organic acids, biotins and the like are generated, so that the palatability and the safety are increased, the utilization rate of the soya bean meal and the nutritive value of a feed are remarkably improved, and the diarrhea rate of piglets can be remarkably reduced.

Description

technical field [0001] The invention belongs to the field of biological enzymolysis and fermentation, and relates to a preparation method of peptides, in particular to a compound probiotic peptide and its preparation method and application. Background technique [0002] As an important plant protein source, soybean meal has important application and research value. However, the presence of anti-nutritional substances such as protease inhibitors, lectins, soybean antigenic proteins, urease, phytic acid, and soybean oligosaccharides in soybeans not only greatly hinders the digestion, absorption and utilization of nutrients by animals, but also seriously Harmful to the health and growth of animals. Soybean antigenic protein is a soybean antinutritional factor with the highest residual amount and the strongest antinutritional effect in soybean processed products. It can cause allergic reactions in the intestinal tract and other organs of infants, mice, piglets, calves and other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 李慧芬张大伟马成
Owner 九源岳阳生物科技饲料有限公司
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