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Haemophilus paragallinarum, inactivated vaccine and preparation method of inactivated vaccine

A technology of Haemophilus gallinarum and inactivated vaccines, applied in the field of microbial preparations, can solve the problems of recurrence of Haemophilus paragallinarum, poor prevention effect of sick chickens, drug-resistant strains, etc., and achieve high safety evaluation coefficient and good immune effect , Good antigenic effect

Inactive Publication Date: 2014-12-17
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of Haemophilus paragallinarum is mainly based on antibiotics, but the use of antibiotics often leads to the emergence of drug-resistant strains, resulting in the recurrence of Haemophilus paragallinarum in the same flock
Therefore, research on Haemophilus paragallinarum vaccine has become a hot spot, but the current Haemophilus paragallinarum vaccine has poor preventive effect on some sick chickens

Method used

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  • Haemophilus paragallinarum, inactivated vaccine and preparation method of inactivated vaccine
  • Haemophilus paragallinarum, inactivated vaccine and preparation method of inactivated vaccine
  • Haemophilus paragallinarum, inactivated vaccine and preparation method of inactivated vaccine

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Embodiment 1

[0023] Haemophilus paragallinarum HN-VA strain of the present invention is obtained in the following manner:

[0024] In June 2012, a large number of chickens over 7 weeks old in a chicken factory in Weishi, Zhengzhou suffered multiple infectious diseases characterized by sneezing, runny nose, facial edema, eyelid and swollen palpebral edema, resulting in death of chickens and laying eggs rate drops. Therefore, for the wholesale sick chicks, the pathogenic bacteria were isolated and purified, named HN-VA strain. Through the observation of the HN-VA strain, specific PCR identification and sequencing analysis showed that the HN-VA strain belonged to Haemophilus paragallinarum.

[0025] (1) Treatment of disease material: insert a sterile swab into the deep part of the nasal sinuses of the affected chick to take the secretion as the disease material, then culture it in chicken serum agar broth medium by streaking, culture at 37°C for 48 hours, and then pick a single colony Inocu...

Embodiment 2

[0039] The inactivated vaccine of Haemophilus paragallinarum of the present invention, preparation method is as follows:

[0040] (1) Seed inspection for production: use the first-grade seeds to count the colonies on the agar plate. Bacterial counts were carried out in accordance with the Veterinary Microbiological Products Regulations issued by the Ministry of Agriculture, with 10 -1 , 10 -2 , 10 -3 , 10 -10 Dilute, and then take 0.1mL of the dilution and spread it evenly on the agar plate, make 2 plates for each dilution, place it in a 37°C incubator and cultivate it for 16-20h, the average number of 2 plates is the number of bacteria at the dilution. The bacterial solution cultivated in nutrient broth is used as a control, and the number of colonies per milliliter is not less than 1.5 billion square meters and can be used for production.

[0041] (2) Preparation of inactivated vaccine: The seeds used for production are expanded and cultivated by 10,000 times, the medium...

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Abstract

The invention discloses haemophilus paragallinarum, an activated vaccine, and a preparation method of the activated vaccine. The provided haemophilus paragallinarum is actually the HN-VA strain of haemophilus paragallinarum, and the HN-VA strain has been preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.7211. For the first time, the HN-VA strain of haemophilus paragallinarum is adopted to prepare an activated vaccine for preventing chicken infectious rhinitis. The research and preparation of the inactivated vaccine effectively solve the problems that the chicken infectious rhinitis is hard to prevent, and the happening rate and death rate of the disease are high; moreover the inactivated vaccine will not generate any adverse reaction to chicken, the experimental safety is high, a high-titer neutralizing antibody will generate in the chicken after vaccination, and the immunity effect is good.

Description

technical field [0001] The invention relates to a Haemophilus paragallinarum, and the inactivated vaccine of the Haemophilus paragallinarum and a preparation method thereof belong to the field of microbial preparations. Background technique [0002] Haemophilus paragallinarum (Haemophilus paragallinarum) is a pathogenic bacterium that causes acute upper respiratory tract disease in chickens. The disease was first discovered in Poland and the United States, and later frequently occurred in other countries. Since 1980, there have been many reports of this disease in my country. Commonly known as chicken infectious rhinitis, this respiratory disease is clinically characterized by facial edema, sinusitis, and tearing. It can cause a decrease in egg production of laying hens, hindered growth of reared chickens, delayed laying period, and decreased weight gain of broiler chickens. Cause huge economic loss to poultry industry. At present, the treatment of Haemophilus paragallina...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
Inventor 王卫芳徐进陈建晖
Owner ZHENGZHOU HOUYI PHARMA
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