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Pseudomonas aeruginosa vaccine and preparation method thereof

A Pseudomonas aeruginosa and vaccine technology, applied in biochemical equipment and methods, antibacterial drugs, pharmaceutical formulations, etc., can solve the problems of high toxicity of the body, low vaccine protection effect, difficult to cure infection, etc., and achieve enhanced immune response Effect

Active Publication Date: 2014-12-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems that the existing whole-cell vaccines have low protective efficacy, high toxicity to the body, difficulty in curing infection and unable to meet clinical immune requirements, and provide a new whole-cell vaccine of Pseudomonas aeruginosa Vaccine and its preparation method

Method used

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  • Pseudomonas aeruginosa vaccine and preparation method thereof
  • Pseudomonas aeruginosa vaccine and preparation method thereof
  • Pseudomonas aeruginosa vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Embodiment 1, preparation and efficacy test of Pseudomonas aeruginosa vaccine of the present invention

[0068] Select the standard strain of Pseudomonas aeruginosa ATCC 27853, use LB medium to culture and proliferate on a bacterial shaker at 37°C and 220r / min for 16 hours, and use 0.5 McFarland turbidity to detect the concentration, so that the bacteria reach about 10 11 number of bacteria. Collect the bacterial culture medium, centrifuge at 3500r / min for 10min, collect the centrifuged sediment and resuspend it with sterile saline, then centrifuge again according to the above conditions, and repeat 3 times. Resuspend the bacterial centrifuged sediment with sterile normal saline, put it in a 50ml BD tube, place it under the center of the radioactive source of the X-ray irradiator for irradiation, and dilute the irradiated bacteria with sterile normal saline for 100 After doubling, spread evenly on the LB agar plate with a bacteria-coating stick, and incubate at 37°C fo...

Embodiment 2

[0071] Embodiment two, preparation and efficacy test of Pseudomonas aeruginosa vaccine of the present invention

[0072] Select the standard strain of Pseudomonas aeruginosa ATCC27853, use LB medium to culture and proliferate on a bacterial shaker at 37°C and 220r / min for 16 hours, and use 0.5 McFarland turbidity to detect the concentration, so that the bacteria reach about 10 11 number of bacteria. Collect the bacterial culture medium, centrifuge at 3500r / min for 10min, collect the centrifuged sediment and resuspend it with sterile saline, then centrifuge again according to the above conditions, and repeat 3 times. After resuspending the bacterial centrifuged sediment with sterile physiological saline, put it in a 50ml BD tube, place it under the center of the radioactive source of the X-ray irradiator, and irradiate it under the condition of 8Gy / min for a total of 470 minutes of intermittent irradiation, (20 Minutes, stop after 5 minutes and continue, repeat the above cycle...

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Abstract

The invention relates to the technical field of biology and specifically relates to a pseudomonas aeruginosa vaccine and a preparation method thereof. The pseudomonas aeruginosa vaccine is used for solving the technical problem that the existing pseudomonas aeruginosa vaccine at the development and clinical test stages are low in protective efficacy, high in toxicity to organisms and incapable of meeting the requirements of clinical immunology due to failure in curing infection. The technical scheme adopted to solve the technical problem is as follows: a preparation method of a novel vaccine is provided. The preparation method comprises the steps of firstly obtaining pseudomonas aeruginosa bacteria and then irradiating the bacteria by use of rays to obtain the pseudomonas aeruginosa vaccine. The pseudomonas aeruginosa vaccine is used for either preventing diseases or treating diseases, and has good application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacterial vaccine and a preparation method thereof. technical background [0002] Pseudomonas aeruginosa (P.aeruginosa), also known as Pseudomonas aeruginosa, is a common opportunistic pathogen, which has strong resistance to various external environments and inactivation measures, especially in humid environments for a long time. Survive. The bacterium exists in many places such as soil, water, air, human and animal skin, mucous membrane, intestinal tract and upper respiratory tract. Its pathogenic feature is that it causes secondary infection, which mostly occurs in patients with reduced body resistance, such as large area burns, long-term use of immunosuppressants, tumors, and patients with hypoproteinemia. Infections caused by Pseudomonas aeruginosa can occur in any part of the human body, and clinically common skin and subcutaneous tissue infections, otitis media, meningitis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/104A61P31/04C12N13/00
Inventor 魏于全李燕燕王震玲
Owner SICHUAN UNIV
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