A kind of tissue culture device and micro-cuttage rapid propagation method of Betula glabra
A tissue culture device and micro-cutting technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of plant death, inability to maintain plant root systems completely, etc., achieve shortened growth cycle, good germination and rooting conditions, excellent material effect
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Embodiment 1
[0029] figure 1 The structure schematic diagram of the tissue culture device provided by the present invention includes a culture vessel main body 1 and a cover 5, and the culture vessel main body 1 and cover 5 are prior art, and will not be repeated here. Different from the prior art, the present invention is provided with a fixed piece 3 in the container main body 1, and the lower part of the culture vessel main body 1 is a semi-solid medium 2, and the fixed piece 3 is positioned on the upper surface of the semi-solid medium 2, and the fixed piece 3 There is an opening 7 for inserting the plant explant 2, and the explant is fixed on the fixing sheet. The fixed sheet 3 can be made by punching holes with filter paper, or other materials can be used. The fixed sheet needs to meet the following requirements: on the one hand, it can fix the explant, and on the other hand, it will not limit the growth of the plant. Using this device for culture, when the culture medium needs to ...
Embodiment 2
[0032] When adopting the device in embodiment 1 to carry out the rapid propagation of birch miniature cuttings, the operation steps are as follows:
[0033] 1) Prepare a semi-solid medium and pour it into a culture container. After autoclaving, the medium is naturally cooled to a semi-solidified state;
[0034] 2) Place the high-temperature sterilized filter paper on the surface of the semi-solid medium in the culture container on the sterile operating table;
[0035] 3) Select aseptic seedlings of the same clone of Betula glabra with consistent growth, cut off the 3-4 and 5-6 stem segments on the aseptic operating table, and leave half a leaf on the top of each explant;
[0036] 4) Insert the morphological lower end of the explant into the semi-solid medium through the filter paper hole, straighten it, tighten the cover of the culture container, and place it on the culture rack;
[0037]5) Put it into the cultivation room for light cultivation, keep the temperature at 23-27°...
Embodiment 3
[0039] Embodiment 3: Betula glabra semi-solid medium formula
[0040] Using MSB5 semi-solid medium, the specific formula is as follows:
[0041] MSB5 semi-solid medium (1L)
[0042] MS large amount of mother liquor (20×) 50ml
[0043] MS trace stock solution (20×) 50 ml
[0044] MS iron salt mother liquor (20×) 50 ml
[0045] B5 organic mother liquor (100×) 10 ml
[0046] Sucrose 30 g
[0047] Agar powder 2.8 g
[0048] pH adjusted to 5.9
[0049] The specific mother liquor formula is as follows:
[0050] NH 4 NO 3
[0051] FeSO 4 ·7H 2 o
[0052] KI
[0053] Inositol
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