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Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators

A technology of plant growth regulators and growth regulators, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., to achieve the effects of simple operation, breaking through limitations, and reducing production costs

Active Publication Date: 2014-12-10
成都上交致远生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no patents and literature reports related to the periwinkle hypocotyl regeneration plant mentioned in this patent application

Method used

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  • Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators
  • Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators
  • Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators

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Embodiment 1

[0025] Periwinkle seeds (the Pacific cherry red halo variety purchased from Ball Seed Company, USA) were planted in peat vermiculite and perlite culture medium (the ratio of peat, vermiculite and perlite was 6:3:1), and watered weekly once. When the plant enters the florescence, when the plant height is greater than 50cm, the periwinkle plants are treated with different plant growth regulators or mixtures, the spraying amount is 250ml, and the spraying is repeated 2 times, and the periwinkle plants treated with water are used as contrast. After one and two days of treatment, the leaves of the plants were harvested, and the contents of ventolin, vinblastine and vinblastine were determined by high performance liquid chromatography.

[0026] Under the above-mentioned same conditions, use the mixed preparation of 0.1mM ethephon, 0.1mM methyl jasmonic acid, and 0.1mM ethephon+0.1mM methyl jasmonic acid respectively (the configuration of the mixed preparation: first mix ethephon and...

Embodiment 2

[0028] Periwinkle seeds (the Pacific cherry red halo variety purchased from Ball Seed Company, USA) were planted in peat vermiculite and perlite culture medium (the ratio of peat, vermiculite and perlite was 6:3:1), and watered weekly once. When the plant enters the flowering period and the plant height is greater than 50cm, treat the periwinkle plant with different plant growth regulators or mixtures, the spraying amount is 300ml, repeat the spraying twice, and after 48 hours, harvest the leaves of the plant and use high performance liquid chromatography Determination of Wenduolin, vinblastine and vinblastine content.

[0029] Under the same conditions as above, group A (0.1mM ethephon+0.1mM methyljasmonic acid), group B (0.1mM ethephon+0.2mM methyljasmonic acid), group C (0.2mM ethephon+0.1 mM methyl jasmonic acid), the mixed preparation of D group (0.2mM ethephon+0.2mM methyl jasmonic acid) is processed periwinkle plant; The result of 4 experimental groups is as shown in t...

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Abstract

The invention discloses a method for disposing and improving the alkaloid content in catharanthus roseus through plant growth regulators. Catharanthus roseus seeds are put into peat vermiculite perlite culturing media to germinate and grow, after catharanthus roseus enters a flowering phase, different plant growth regulators (ethephon and methyl jasmonic acid) are mixed to form a mixture with the concentration being 0.1 mM, the mixture is sprayed on catharanthus roseus plants, the vindoline content, the catharanthine content and the vincaleukoblastinum content in the catharanthus roseus are generally improved after the processing is conducted one to two days, disposed catharanthus roseus plants are harvested, the output of alkaloids of the catharanthus roseus can be effectively improved, production cost of the catharanthus roseus is reduced, and great significance is achieved on meeting the industrialized production of anticancer alkaloids of the catharanthus roseus.

Description

technical field [0001] The invention relates to a method for increasing the alkaloid content of periwinkle in the field of biotechnology, in particular to a method for increasing the alkaloid content of periwinkle by treating with a plant growth regulator. Background technique [0002] As a medicinal and ornamental plant, Catharanthus roseus (L.) G.Don of the Apocynaceae family contains more than 130 terpenoid indole alkaloids (Terpenoid indole alkaloid, TIA) with biological activity. Vinblastine and Vincristine against a variety of malignant tumors and cancers, their precursors Vindoline and Catharanthine, amoline for lowering blood pressure and anti-aging effect on Alzheimer's disease Syndrome (Alzheimer's disease) has potential curative effect of serpentine and so on. However, the content of TIA in wild-type periwinkle is very low, only one ten-thousandth to one-thousandth of the dry weight of the plant, and affected by tissue specificity, growth cycle and external envir...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01N57/20A01P21/00
Inventor 唐克轩潘琪芳王玉亮孙光新刘品
Owner 成都上交致远生物科技有限公司
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