A kind of functional peptide protein powder and its preparation method and application
A functional peptide and protein powder technology, applied in the field of animal-derived protein feed preparation, can solve the problems of large changes in composition, easy moisture absorption, affecting feeding effect, etc. The effect of improving the hydrolysis efficiency
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[0099] ②Preparation of serine standard solution: 50mg of serine was dissolved in 500ml of deionized water (0.9516meqv / L).
[0100] ③Preparation of sample solution: Xg sample is dissolved in 100ml deionized water, X is 0.1-1.0g sample containing 8%-80% protein.
[0101] ④Standard measurement: Add 400μL of serine standard solution into a stoppered test tube containing 3mL of OPA reagent, shake well, react accurately at room temperature for 2min, and then measure its absorbance at 340nm with a UV spectrophotometer.
[0102] ⑤ Blank measurement: Add 400 μL deionized water to a stoppered test tube containing 3 mL of OPA reagent, shake well, react accurately at room temperature for 2 min, and then measure its absorbance at 340 nm.
[0103] ⑥Sample detection: Add 3 mL of OPA reagent to 0.4 μL of the sample to be tested and mix well, let it stand at room temperature for 2 minutes, and then measure its absorbance at 340 nm.
[0104] ⑦Calculation:
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Embodiment 1
[0119] Example 1 Preparation of functional peptide protein powder E-1
[0120] (1) Preparation of blood cells: Collect fresh duck blood from the slaughterhouse in a safe and hygienic way, and then add 0.6% sodium citrate to the total blood weight to prevent blood coagulation. Transport the duck blood with sodium citrate to the processing plant in the shortest possible time, and centrifuge it with a high-speed centrifuge at a speed of 14,000r / min for 10 minutes. At this time, the blood is divided into upper and lower layers, and the upper layer is light yellow plasma. , stored for other purposes; the lower layer is dark red viscous blood cells.
[0121] (2) Cell wall breaking: add water and hydrochloric acid solution with a concentration of 0.25mol / L to the blood cells obtained by centrifugation to prepare blood cell dilution, the mass ratio of hydrochloric acid solution, blood cells, and water is 1:4:5, and then make the substrate The concentration was maintained at 15g / 100mL...
Embodiment 2
[0128] Example 2 Preparation of functional peptide protein powder E-2
[0129] (1) Preparation of blood cells: Collect fresh duck blood from the slaughterhouse in a safe and hygienic way, and then add 0.8% sodium citrate to the total blood weight to prevent blood coagulation. Transport the duck blood with sodium citrate to the processing plant in the shortest possible time, and centrifuge it with a high-speed centrifuge at a speed of 16,000r / min for 8 minutes. At this time, the blood is divided into upper and lower layers, and the upper layer is light yellow plasma. , stored for other purposes; the lower layer is dark red viscous blood cells.
[0130] (2) Cell wall breaking: add water and hydrochloric acid solution with a concentration of 0.05mol / L to the blood cells obtained by centrifugation to prepare blood cell dilution, the mass ratio of hydrochloric acid solution, blood cells, and water is 1:3:6, and then make the substrate The concentration was maintained at 11.5g / 100m...
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