A detection method of medicine for treating fever in children
A detection method and technology for pediatric fever, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of not reflecting the intrinsic quality of drugs, lack of comprehensiveness, etc., and achieve the effect of shortening the inspection cycle, good repeatability, and comprehensive product quality.
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Embodiment 1-4
[0040] Embodiment 1-4 uses different chromatographic conditions to detect palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate;
[0041] Take appropriate amount of 4 reference substances of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate respectively, put them in a 50mL volumetric flask, add methanol to make each 1mL contain 60μg of palmatine hydrochloride, 80μg of berberine hydrochloride, licorice A mixed solution of 70 μg of glycoside and 200 μg of ammonium glycyrrhizinate was used as a mixed reference solution.
[0042] Linghuang Baoer pills were crushed and passed through a No. 4 sieve, and 0.5g of fine powder was taken, accurately weighed, placed in a stoppered Erlenmeyer flask, accurately added with 25mL of methanol, weighed, treated with ultrasonic (480W, 40KHz) for 30min, let cool, added Make up the lost weight with methanol, shake well, filter with a microporous membrane (0.45 μm), and take...
Embodiment 5
[0054] This example provides an investigation of the linear range of the method.
[0055] Take 10 mg of liquiritin, 10 mg of palmatine hydrochloride, 12 mg of berberine hydrochloride, and 10 mg of ammonium glycyrrhizinate as reference substances, accurately weigh them, put them in the same 50 ml volumetric flask, add methanol to dissolve and dilute to the mark. Accurately draw reference substance solution 1ml, 2ml, 3ml, 5ml, 8ml, 10ml to 10ml volumetric flask, add methanol to dilute to the mark and shake well, according to the method provided in Example 1 of the present invention, sequentially inject and measure. Taking the determination of the peak area as the ordinate, and the concentration of the reference substance solution as the abscissa (μg / ml) to draw a standard curve, the results are shown in Table 3, Figure 2a , 2b , 2c, 2d.
[0056] Table 3 Regression equation, correlation coefficient and linear range of four kinds of reference substances
[0057]
[0058] ...
Embodiment 6
[0060] The precision test that present embodiment provides method:
[0061] Linghuang Baoer pills were crushed and passed through a No. 4 sieve, and 0.5g of fine powder was taken, accurately weighed, placed in a stoppered Erlenmeyer flask, accurately added with 30mL of methanol, weighed, treated with ultrasonic (480W, 40KHz) for 15min, let cool, added Make up the lost weight with methanol, shake well, filter with a microporous membrane (0.45 μm), and take the subsequent filtrate as the test solution.
[0062] Take the solution to be tested, according to the method provided in Example 1 of the present invention, continuously inject 6 times, 5 μ L each time, record the peak areas of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate, and calculate the relative standard deviation , the result is as shown in table 4, and the result shows, adopts the method provided by the invention to detect palmatine hydrochloride, berberine hydrochloride, l...
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