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Method for extracting rutin from pagodatree flower bud

An extraction method and technology of Sophora japonica, which are applied in chemical instruments and methods, preparation of sugar derivatives, production of bulk chemicals, etc., can solve the problems of reduced production efficiency, dissolution of rutin in hot water, long use time, etc., and achieve easy operation. , the effect of shortening the extraction time and increasing the yield

Active Publication Date: 2014-11-05
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods of deactivating enzymes adopted in the prior art mainly include dry steaming, ultraviolet irradiation, 60 Co irradiation, microwave enzyme inactivation, etc. Among them, the dry steaming temperature is high, which will cause the hydrolysis and oxidation of rutin while inactivating the enzyme, and will also cause part of the rutin to dissolve in hot water and be lost; while ultraviolet radiation can effectively inactivate the enzyme. , but it takes a long time and the cost is high, which reduces the production efficiency

Method used

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  • Method for extracting rutin from pagodatree flower bud
  • Method for extracting rutin from pagodatree flower bud
  • Method for extracting rutin from pagodatree flower bud

Examples

Experimental program
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Embodiment 1

[0016] Crush 20g of Huai Mi to 30 mesh, add acid water with a temperature of 35°C and pH=4~5, and after stirring, add supercritical CO 2 In the extraction device, treat at 30MPa and 30-40°C for 40min, then wash twice with acid water at 0-5°C and pH=4-5 and then spin dry; add 4% borax to the dried Huaimi granules , 1% Na 2 SO 3 And 25 times of 60% ethanol, add sodium bicarbonate to adjust pH=9~10, and ultrasonic extraction for 30min under the conditions of 30kHz and 40℃, protected from light, and then filtered, the filtrate is protected from light at 30~50℃ for later use; Ultrasonic extraction is performed once under the same conditions, and the filtrate obtained from the two extractions is combined. 5% activated carbon is added to it, and stirred for 10 minutes at 40°C in the dark. The filtrate after filtering and removing carbon is acidified to pH=4~5, using 30kHz ultrasonic Treat it in the dark at 0~5℃ for 30min, leave it to crystallize, and filter with suction to obtain the c...

Embodiment 2

[0018] Crush 30g of Huai Mi to 50 mesh, add acid water with a temperature of 40°C and pH=4~5, and after stirring, add supercritical CO 2 In the extraction device, treat at 30MPa and 30-40°C for 40min, then wash twice with acid water at 0-5°C and pH=4-5 and then spin dry; add 3% borax to the dried Huaimi granules , 2% Na 2 SO 3 And 20 times of 60% ethanol, adjust pH=9~10 with sodium bicarbonate, extract 30min under the conditions of 25kHz, 35℃ and avoid light, then filter, and keep the filtrate away from light at 30~50℃. Extract once under the same conditions as above, combine the filtrate obtained from the two extractions, add 5% activated carbon to it, stir for 15 minutes at 40°C in the dark, acidify the filtrate after filtering the carbon to pH=4~5, adopt 20~30kHz Ultrasonic treatment at 0~5℃ in the dark for 30min, standing to crystallize, and suction filtration to obtain crude rutin; place the crude rutin in an oven, dry at 40℃ until there is no alcohol, and then gradually he...

Embodiment 3

[0020] Crush 50g of Huai Mi to 20 mesh, add acid water with a temperature of 35°C and pH=4 to 5, and after stirring, add supercritical CO 2 In the extraction device, treat at 30MPa and 30-40°C for 40min, then wash twice with acid water at 5°C and pH=4-5 and then spin dry; add the spin-dried Huaimi granules to 4% borax, 1 %Na 2 SO 3 And 30 times 70% ethanol, adjust pH=9~10 with sodium bicarbonate, extract 30min under the conditions of 30kHz, 40℃ and avoid light, then filter, and keep the filtrate away from light at 30~50℃. Ultrasonic extraction is performed once under the same conditions, and the filtrate obtained from the two extractions is combined. 5% activated carbon is added to it, and stirred for 15 minutes at 40°C in the dark. Acidify the filtrate after filtering and removing carbon to pH=4~5, using 20~30kHz Ultrasonic treatment at 0~5℃ in the dark for 30min, standing for crystallization, and suction filtration to obtain the crude rutin; put the crude rutin in an oven, dry...

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Abstract

The invention discloses a new method for extracting rutin from pagodatree flower bud. The method comprises the following steps: smashing the pagodatree flower bud, killing enzyme by adopting a supercritical CO2 method, carrying out ultrasonic-assisted extraction by using 60-70% ethyl alcohol under a certain condition to obtain an extracting solution, standing and crystallizing the extracting solution, carrying out suction filtration to obtain rutin crude products, refining the rutin crude products by using n-butanol, drying at the temperature of 130 DEG C to obtain rutin products with the purity being above 97% and the yield being above 22%. The method disclosed by the invention is simple and convenient to operate and high in extracting efficiency and is suitable for production practice.

Description

Technical field [0001] This application relates to a method for extracting rutin from Sophora japonica. Background technique [0002] Sophora japonica is the dried flowers and flower buds of the legume locust tree. It has been used as medicine for more than 2,000 years. The "Shen Nong's Materia Medica" lists it as the top grade. The rutin contained in Sophorae is about 8%-28% (depending on the origin of Sophorae and the time of collection). Rutin, also known as rutin and purple quercetin, belongs to the flavonoid glycoside compound. It has the effects of cooling blood to stop bleeding, clearing the liver and purging fire, reducing the permeability and fragility of capillaries, promoting cell proliferation and preventing blood cell aggregation. Anti-inflammatory, anti-allergic, diuretic, antitussive, and hypolipidemic effects. Rutin has been clinically used as an adjuvant treatment of hypertension, to prevent other hemorrhages caused by rutin deficiency, and to prevent and treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCY02P20/54
Inventor 高瑞白易张成亮赵永强贾洪涛
Owner 内蒙古昶辉生物科技股份有限公司
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