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Method and kit for diagnosing liver cancer by use of the ratio of change in serum miRNA quantity

A kit and liver cancer technology, applied in the fields of immunology and molecular biology, can solve problems such as diagnostic errors, instability, and poor repeatability, and achieve the effect of improving accuracy

Inactive Publication Date: 2014-10-29
常州百代生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It only judges the diagnosis of liver cancer by the CT value of a single miRNA, but there are still certain diagnostic errors
Obviously, this invention patent application did not take into account the differences in the absolute amount of miRNA expression among individuals, that is, the factors of tumor tissue size and development stage and their influence on the expression amount of miRNA in serum
In addition, the extraction and quantification methods of miRNA are diverse, unstable, and poor in repeatability, resulting in poor comparability of results from different laboratories.
Taking these factors into account, the assay method of this application has certain limitations

Method used

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  • Method and kit for diagnosing liver cancer by use of the ratio of change in serum miRNA quantity
  • Method and kit for diagnosing liver cancer by use of the ratio of change in serum miRNA quantity
  • Method and kit for diagnosing liver cancer by use of the ratio of change in serum miRNA quantity

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Experimental program
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Effect test

Embodiment 1

[0057] The fundamental purpose of the present invention is to diagnose liver cancer by measuring the ratio of two or more groups of miRNAs, so as to improve the accuracy of liver cancer diagnosis. And measure the amount of every group of miRNA then can adopt different methods, the embodiment one of the method for measuring miRNA amount of the present invention is as follows:

[0058] Step 1: Blood samples were collected from the hospital in advance and divided into a healthy group and a confirmed liver cancer group. Divide each group into two groups, which are healthy group: A1, A2, and liver cancer group: B1, B2. The following steps 2, 3, and 4 are used to measure the amount of miRNA in each group of samples.

[0059] Step 2: Take 2ml of peripheral blood from each group of samples, place it at room temperature for 1 hour, and centrifuge at 1000g for 10 minutes. Then, the blood was drawn and transferred to a 1.5ml tube and centrifuged at 16000g for 20 minutes to remov...

comparative approach 1

[0100] Comparison scheme 1 of the present invention: direct comparison of two sets of data on the decrease degree of Mir-122a serum. Such as figure 1 As shown, the amount of Mir-122a in the test group 1 is between 0 and 1, and the amount of Mir-122a in the test group 2 is between 2 and 4. Well diagnosed liver cancer.

comparative approach 2

[0101] Comparison scheme 2 of the present invention: direct comparison of two sets of data on the degree of increase in Mir-222 serum. Such as figure 2 As shown, the amount of Mir-222 in the test group 1 is between 0.8 and 2, and the amount of Mir-222 in the test group 2 is between 4 and 8. The data of the two groups are also very different, so only one Mir-222 value Can not be a good diagnosis of liver cancer.

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Abstract

The invention relates to the technical field of immunology and molecular biology, and particularly relates to a method for diagnosing liver cancer by use of the ratio of change in serum miRNA quantity. The method comprises the following steps: collecting a blood sample; separating Mir-RNA; performing quantitative determination of two or more Mir-RNAs in blood; calculating the proportion of the two or more Mir-RNAs according to the determination result; and measuring the proportion range of multiple groups of Mir-RNAs to determine the index of Mir-RNA change as the diagnosis basis. In the method provided by the invention, the liver cancer is diagnosed by measuring two or more Mir-RNAs and the ratio thereof, and the accuracy in liver cancer diagnosis can be effectively increased.

Description

Technical field [0001] The invention involves immunology and molecular biology technology, especially a method and kit that uses the amount of serum MiRNA amount to diagnose liver cancer. Background technique [0002] Hepatocytate (HCC) is one of the most common malignant tumors, and liver cancer survival rate is very low.In China and 1 Asian country, most liver cancer comes from hepatitis B virus.The further understanding of cancer biology and technological progress have been able to sign a variety of potential biomarkers of liver cancer.Early diagnosis of liver cancer is the key to current treatment and extending survival rate.The detection of biomarkers not only helps prognosis or recurrence prediction, but also help determine treatment methods or intervention measures.At present, the diagnosis of liver cancer mainly depends on the amount of AFP of serum, but its positive diagnosis rate is very low, and the specificity has certain limitations. [0003] The most commonly used b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 崔坤元夏国庆
Owner 常州百代生物科技股份有限公司
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