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Preparation method of calcium-strontium/gelatin biomimetic coating modified artificial ligament

An artificial ligament and gelatin technology, applied in the field of biomedicine, can solve the problems of poor repeatability of experimental results, low rate of osteogenic differentiation, uneven coating, etc., to improve biocompatibility and increase the chance of tendon-bone healing , Facilitate the effect of cell adhesion

Active Publication Date: 2014-10-29
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research results also show that the artificial direct coating method has problems such as uneven coating, lack of stability, poor reproducibility of experimental results, and low osteogenic differentiation rate.

Method used

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  • Preparation method of calcium-strontium/gelatin biomimetic coating modified artificial ligament
  • Preparation method of calcium-strontium/gelatin biomimetic coating modified artificial ligament
  • Preparation method of calcium-strontium/gelatin biomimetic coating modified artificial ligament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Under background vacuum 5 x 10 10-2 Pa, under the condition of power 200W, use H 2 Plasma treated the PET artificial ligament for 8 minutes, then soaked in 10mM acrylic acid monomer grafting solution for 2 hours, and finally washed with pure water and dried in vacuum for later use;

[0038] Add 2g of bone gelatin particles to 100ml deionized water to swell, then heat to 55°C to form a 2wt.% bone gelatin solution with a molar concentration of ; then add 6ml of 40mM CaCO 3 and 6ml 20mM SrNO 3Add it to the gelatin solution, adjust the pH value to 7, and stir at 25°C for 10 minutes to obtain a uniformly mixed solution; soak the pretreated artificial ligament in this mixed solution to fully combine the free Ca and Sr ions and gelatin with the ligament , take the ligament out of the filter paper to dry, and finally soak in 500ml 60mM freshly prepared Na 2 HPO 4 The solution was irradiated by sunlight for 20 minutes, and the above steps were repeated 20 times to obtain a n...

Embodiment 2

[0044] 8 x 10 in background vacuum 10-2 Pa, Ar under the condition of power 400W 2 Plasma treated the PET artificial ligament for 20 minutes, then soaked in 100mM polyethylene glycol monomer grafting solution for 3 hours, and finally washed with pure water and dried in vacuum for later use;

[0045] Add 10 g of bone gelatin particles to 100 ml of deionized water to swell, then heat to 50 ° C to form a 10% bone gelatin solution, and then add 6 ml of 120 mM CaCO 3 Add 6ml of 30mM strontium dichloride to the gelatin solution, adjust the pH value to 8, and stir for 20 minutes at 25 degrees Celsius to obtain a uniformly mixed solution; soak the pretreated artificial ligament in the mixed solution, and stir for 45 minutes to react. Make the free Ca and Sr ions and gelatin fully combined with the ligament, take out the filter paper to dry the ligament, and finally soak it in 600ml 90mM freshly prepared sodium phosphate solution, expose it to simulated sunlight for 30min, and repeat ...

Embodiment 3

[0047] Add 10g of bone gelatin particles to 200ml of deionized water to swell, then heat to 60°C to form a 5% bone gelatin solution, add 6ml of 300mM mixed solution of calcium nitrate and calcium carbonate, and 6ml of mixed solution of 50mM strontium nitrate and strontium chloride In 5% gelatin solution, adjust the pH value to 7.4, stir at 25°C for 30 minutes to obtain a well-mixed solution; then vacuum 7×10 10-2 Pa, under the condition of power 350W, use H 2 Plasma treated the PET artificial ligament for 15 minutes, then soaked it in 55mM heparin monomer grafting solution for 6 hours, and finally washed it with pure water and dried it in vacuum; soaked the pretreated artificial ligament in the mixed solution, stirred and reacted for 50 minutes, so that the free Ca and Sr ions and gelatin are fully combined with the ligament, the ligament is taken out of the filter paper to dry, and finally soaked in 250ml 75mM freshly prepared mixed solution of potassium dihydrogen phosphate ...

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Abstract

The invention discloses a preparation method of a calcium-strontium / gelatin biomimetic coating modified artificial ligament. The preparation method comprises the following steps: adding strontium salt and calcium salt into a gelatin solution, stirring so as to be fully dissolved, to obtain an evenly mixed solution; immersing a plasma-treated surface modified artificial ligament into the solution, adding phosphate, and stirring to form a mixed solution, irradiating under sunlight or simulated sunlight for 20-60 minutes, and sucking dry with filter paper; repeating the third step until the preset mineralizing circulation times are achieved, thus obtaining the calcium-strontium / gelatin biomimetic coating modified artificial ligament. According to the method, the biological mineralization mechanism of natural bone is simulated, an inorganic salt layer of apatite is deposited by taking natural high polymer or gelatin as a matrix or a template, a coating is compounded on the surface of the material, so that the artificial ligament carries nanoscale strontium substituted hydroxyapatite, so that the biocompatibility and osteoconduction of the material can be improved, and the material can meet the clinically medical requirements on the aspects of bioactivity and mechanical performance.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a preparation method of calcium strontium / gelatin bionic coating modified artificial ligament. Background technique [0002] Polyethylene terephthalate (PET) artificial ligaments have been widely used clinically as grafts for the reconstruction of cruciate ligament injuries of the knee joint, and the short-term curative effect and safety are relatively satisfactory, but there are still poor tendon-bone healing And concurrent synovitis and other problems. Therefore, it is particularly important to improve the biocompatibility of PET materials and promote the tendon-bone healing of the artificial ligament. [0003] Surface coating treatment of PET artificial ligament grafts to improve the hydrophilicity, biocompatibility and osteoconductivity of biomaterials has become the focus of the development of orthopedic biomaterials. Reasonable use of multiple components to compound biomedical mat...

Claims

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Application Information

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IPC IPC(8): C08J7/04C09D189/00C09D7/12C08J7/12C08L67/02A61L27/34A61L27/30A61L27/18
Inventor 张鹏陈世益陈俊蒋佳陈天午万方
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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