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Fast manufacturing technology for establishing golden hamster atherosclerotic model

A technology for atherosclerosis and golden hamster, applied in the field of medicine, can solve the problems of long time-consuming, poor practicability and high cost of the model, and achieve the effects of low cost, shortening the research process, and reducing the cost of animal feeding.

Inactive Publication Date: 2014-10-15
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Studies have shown that hamsters can form atherosclerotic plaques at the 10th month of feeding with a diet containing 3% cholesterol and 15% fat. High, less practical

Method used

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  • Fast manufacturing technology for establishing golden hamster atherosclerotic model
  • Fast manufacturing technology for establishing golden hamster atherosclerotic model
  • Fast manufacturing technology for establishing golden hamster atherosclerotic model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Establishment of an atherosclerosis model in Syrian golden hamsters fed with high-fat diet.

[0020] Material

[0021] [Animal] Syrian golden hamster (professional name: Vr:LVG(SYR))

[0022] [Feed] See the table below for the feed formula

[0023] Table 1: Animal model feed formulation table

[0024]

[0025]

[0026] 1Referenced in Open research diets, D1245 and D12450B.

[0027] 2S10001,mineral mix for AIN-76rodent diet(17).

[0028] 3V10001, vitamin mix for AIN-76A rodent diet (17).

[0029] method

[0030] [breeding of animals]

[0031] Syrian golden hamsters (LVG hamster, sourced from Charles River Laboratories) were fed adaptively for one week, randomly divided into normal group and model group according to body weight, and kept in a suitable environment (breeding temperature 21±2°C, relative humidity 30-70%) , photoperiod 14 / 10 hours), 4 animals / cage, the normal group was given the normal group feed, the atherosclerosis model group was gi...

Embodiment 2

[0035] Example 2: Blood Lipid Dynamics Formed in the Syrian Golden Hamster Model of Atherosclerosis

[0036] Material

[0037] [Animal] Syrian golden hamster (professional name: Vr:LVG(SYR))

[0038] [Feed] See Table 1 for feed formula

[0039] [Main Reagents] Total Cholesterol Determination Kit (Dry Powder with Buffer) (Sinobiological Beikong Biological Reagents Co., Ltd.)

[0040] Total triglyceride kit (Sinobiological Beikong Biological Reagents Co., Ltd.)

[0041] Direct high-density cholesterol assay kit (Sinobiological Beikong Biological Reagents Co., Ltd.)

[0042] Direct LDL Cholesterol Assay Kit (Sinobiological Beikong Biological Reagents Co., Ltd.)

[0043] [Main instrument] SectraMaxTM190 microplate reader

[0044] method

[0045] [breeding of animals]

[0046] Syrian golden hamsters (LVG hamster, sourced from Charles River Laboratories) were fed adaptively for one week, randomly divided into normal group and model group according to body weight, and kept in ...

Embodiment 3

[0051] Example 3: HE staining analysis of the aortic arch in the Syrian golden hamster model of atherosclerosis

[0052] Material

[0053] [Animal] Syrian golden hamster (professional name: Vr:LVG(SYR))

[0054] [Feed] See Table 1 for feed formula

[0055] [Main instrument] Upright microscope Eclipse80i (Nikon Corporation, Japan)

[0056] [Reagent preparation]

[0057] A: 0.5-1% eosin alcohol solution: weigh 0.5-1g eosin Y, add a small amount of distilled water to dissolve it, then add glacial acetic acid dropwise until it becomes a paste. Filter with filter paper, dry the filter residue in an oven, and dissolve it with 100 ml of 95% absolute ethanol.

[0058] B: Formula of hematoxylin dye solution (1000ml prepared, adjusted according to demand): 2g hematoxylin, 33ml absolute ethanol, 50g aluminum potassium sulfate, 667ml distilled water, 0.4g sodium iodate, 40ml glacial acetic acid, 300ml glycerin

[0059]Preparation method: Dissolve hematoxylin in absolute ethanol, then...

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Abstract

The invention discloses a fast simple low-cost manufacturing technology for establishing an atherosclerotic model. The atherosclerotic model is established by feeding high fat diet with unique formula to Syria golden hamster for 5 months, the modeling time is 5 months shorter than the time for forming atherosclerotic plaque on the same species on an international level. The technical scheme provided by the invention can rapidly establish a blood lipid dynamic graph and ultrasound imaging characteristics of the atherosclerotic model and blood vessel arteriosclerosis model characteristics, and is conducive to shortening the research process of atherosclerotic experimental model and innovative drug preclinical evaluation, and provides the reference standard for the model manufacturing technique.

Description

technical field [0001] The invention discloses a fast, simple and low-cost method for rapidly making an atherosclerosis model, which belongs to the technical field of medicine. Background technique [0002] Animals currently used to establish atherosclerosis models include miniature pigs, rabbits, guinea pigs, rats, mice, etc., but they have their own shortcomings. Finding more animal models is conducive to improving the multiple aspect research. Hamsters are highly sensitive to lipid damage. As early as the 1880s, hamsters have been used in the field of lipid metabolism research. Compared with other animal models, the hamster atherosclerosis model has the following advantages : (1) Low cost, small size, easy to breed and raise; (2) Preliminarily confirmed that it is similar to the pathological process of human atherosclerosis; (3) Endogenous cholesterol synthesis is low and has cholesterol lipid transfer protein activity; (4) Influenced by Body-mediated low-density lipopr...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/10A23K1/14A23K1/175A23K1/18A01K67/027A23K20/105A23K20/147A23K20/158A23K20/163A23K50/50
Inventor 朱海波杨康敏杨柳王晓良
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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