A method for regenerating the testis of superrealgar catfish

Abstract

The invention relates to a pelteobagrus fulvidraco male testis regeneration method. The method includes the following steps: immersing dissection tools and stitching tools in absolute ethyl alcohol for disinfection; anesthetizing pelteobagrus fulvidraco male; dissecting the abdominal cavity along the abdomen center line with a dissecting knife, wherein the length of a dissected opening is 2-3cm; after the abdominal cavity is dissected, using tweezers to open the abdominal cavity, after the testis is fully exposed, taking out part of the testis with the tweezers and bent-head scissors, and reserving a spermatogenic area of the testis in an experimental fish; adopting a continuous suture method for stitch the peritoneum, the abdominal muscle and the skin; shifting the postoperative experimental fish into fresh and clean water to enable the experimental fish to recover from the anesthesia state; after the experimental fish is domesticated and cultivated for a period of time, and when the spermatogenic area of the testis can reproduce sperms, taking a new testis of the experimental fish. By the method, misfortune of slaughtering of parent pelteobagrus fulvidraco male can be avoided, reproduction can be realized by the method, and accordingly utilization efficiency of the pelteobagrus fulvidraco male is improved.

Description

technical field [0001] The invention relates to a method for regenerating the testis of superrealgar catfish. Background technique [0002] Yellow catfish is an important freshwater fish species. According to the "2013 China Fishery Statistical Yearbook", the output of yellow catfish farming reached 257,000 tons in 2012, and the demand for seedlings is estimated to reach 15 billion. Under the same breeding conditions, the growth rate of male yellow catfish is more than 30% faster than that of female siblings. Therefore, the inventor invented a method to continuously produce all male yellow catfish (patent number: ZL200610166518.8) using sex reversal and gynogenesis, established a super male yellow catfish breeding population (patent number: ZL200810197519.8) and rapidly improved YY The method of breeding line of super realgar catfish (application number: 201410057631.7). However, in the internal structure of the testis of the yellow catfish, the seminal lobules are arrange...

AI Technical Summary

Problems solved by technology

Moreover, the seminal vesicle of the yellow catfish is highly branched, and it is difficult to squeeze out the sperm from the capsule with external force after the sperm enters, so that the semen of the mature yellow catfish cannot be squeezed out by external force

Therefore, when artificially breeding or using in experiments, the super realgar catfish must be killed and the sperm collected by laparotomy. The disadvantage of this method is that on the one hand, the cultivation of super realgar catfish is complicated and takes a long time, and it takes at least 2 years to reach sexual maturity. Moreover, the testis can only be used once, and the efficiency is low, thereby increasing the cost of artificial reproduction and scientific research; on the other hand, the parents used in breeding or experiments every year are different super realgar catfish, and the produced fry must be sampled and tested , increase the input of manpower and material resources, and the seed quality cannot be guaranteed

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