Novel pure culture of Auricularia auricula and artificial cultivation method thereof

A technology of artificial cultivation and black fungus, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as no research reports, and achieve obvious effects of lowering blood lipids, high economic benefits, and obvious effects

Active Publication Date: 2014-10-15
GUANGDONG INST OF MICROORGANISM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After research, there is n...

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  • Novel pure culture of Auricularia auricula and artificial cultivation method thereof
  • Novel pure culture of Auricularia auricula and artificial cultivation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] The artificial cultivation method of black fungus new strain CGMCC NO.8708, comprises the following steps:

[0033] (1) Making the mother species: collect the isolated fungus black fungus MCC126001, aseptically connect the strain to the slope of the mother species culture medium, and place it in a 25°C incubator for constant temperature and dark cultivation until the mycelia cover the slope and then produce mother species,

[0034] (2) Making the original seed: Aseptically insert the production mother seed into the original seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the original seed after the mycelium is full of material;

[0035] (3) Making production species: insert the original species aseptically into the production seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the production species after the mycelium is full of material;

[0036] (4) Cultivation: The commonly...

Embodiment 2

[0046] Analysis of the active components of the new black fungus strain CGMCC NO.8708

[0047] In this embodiment, 5 different samples including the new bacterial strain of the present invention are used to analyze the active ingredients. The 5 samples are respectively as follows. The active ingredient analysis includes: protein content, total sugar content, calcium content, iron content , Zinc content, phosphorus content, potassium content, magnesium content.

[0048] The five samples are as follows:

[0049] Sample 1: Northeast autumn fungus (Yuanbao No. 2), the cultivation time is inoculated in May, and the ears are grown in July, and the cultivation location is Dongning County, Heilongjiang Province;

[0050] Sample 2: Northeast black fungus (Yuanbao No. 2), the cultivation time is inoculated in January, and the ears are grown in March, and the cultivation location is Dongning County, Heilongjiang Province;

[0051] Sample 3: Tibetan wild fungus No. 6, the cultivation ti...

Embodiment 3

[0064] Analysis of blood lipid-lowering function of a new black fungus strain MCC126001 (CGMCC NO.8708)

[0065] Black fungus is considered in traditional medicine as a kind of edible fungus with strong blood lipid-lowering effect. Therefore, in this embodiment, we choose blood lipid-lowering animal experiments to test the different effects between several black fungus varieties.

[0066] The experimental animal of this embodiment adopts healthy adult male SD rats (purchased from Animal Experiment Center of Guangdong Province), with a body weight of about 200 grams. After adaptive feeding for 1 week, 6 rats were given maintenance feed as a blank control group. Eighteen rats were given high-fat diet as the model group. After 2 weeks, blood was collected from all rats to determine the levels of serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) to detect the modeling situation . After ...

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Abstract

The invention relates to Auricularia auricula and an artificial cultivation method thereof. The novel pure culture of Auricularia auricular is Auricularia auricula (L.exHook.) Underw) MCC126001, CGMCC (China General Microbiological Culture Collection Center) No. 8708. The invention also discloses an artificial cultivation method of the novel pure culture of Auricularia auricular CGMCC No.8708. The artificial cultivation method comprises the following steps of after separation of the pure culture, production of a stock culture and a production culture, cultivation, emergency management and growth period management. The invention has the beneficial effects that the invention provides the novel pure culture of Auricularia auricular coming from Cyclobalanopsis glauca trees at a Shahe township, Jinzhai County, Lu'an City, Anhui, and the invention also provides the artificial cultivation method of the novel pure culture. The test proved that the novel pure culture of Auricularia auricular has high contents of active ingredients such as calcium, zinc and magnesium, so that the hypolipidemic effect is obvious, and thus the pure culture is good for the health, and has very high economic efficiency.

Description

technical field [0001] The invention relates to a new strain of black fungus and its artificial cultivation method. Background technique [0002] Black fungus Auricularia auricula (L.exHook.) Underw, belongs to Basidiomycotina, Basidiomycetes, Auricularia, Auriculariaceae, Auricularia, aliased as fungus, black fungus, fine fungus. It is a well-known mountain delicacy, an edible, medicinal, and tonic fungus that can be used as both food and medicine. It is also a traditional food and export trade food in my country. Black fungus polysaccharide has very important pharmacological effects, such as regulating immune function, lowering blood fat, antithrombotic, antiaging, antitumor, anticoagulant and so on. [0003] China is the main producer of fungus, and the production areas are mainly distributed in Guangxi, Yunnan, Guizhou, Sichuan, Hubei, Heilongjiang, Jilin and other places. Among them, Suiyang Town, Dongning County, Heilongjiang Province is the largest black fungus base ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 胡惠萍谢意珍黄龙花丁银润刘远超曹仁润
Owner GUANGDONG INST OF MICROORGANISM
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