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Novel method for preparing calculus bovis factitius and raw materials thereof

A technology of artificial bezoar and taurine, which is applied in the direction of unknown raw materials, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as difficult to meet artificial bezoar, low active ingredients, and limited output

Inactive Publication Date: 2014-10-08
张隶军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been improved many times afterwards, it is still far from the composition and efficacy of natural bezoar, and there are endless criticisms at home and abroad. So far, artificially produced bezoar still has problems such as extremely low yield and lower content of active ingredients than natural bezoar.
[0004] The bilirubin preparation methods prepared by the traditional bilirubin preparation process include calcium salt method, ion exchange resin method, bacterial enzyme method, etc. However, the bilirubin produced by these methods is of poor quality, limited yield, and It is difficult to effectively meet the demand for artificial bezoar

Method used

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  • Novel method for preparing calculus bovis factitius and raw materials thereof
  • Novel method for preparing calculus bovis factitius and raw materials thereof
  • Novel method for preparing calculus bovis factitius and raw materials thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: At first prepare bilirubin calcium, comprise the following steps:

[0035] The first step: mix pig blood or cow blood with 4% sodium citrate according to the mass ratio of 200:1, mix well, and then let stand for 12 hours to obtain hemoglobin solution;

[0036] Step 2: After adjusting the above solution to PH2.0 with dilute hydrochloric acid, heat it at 60 degrees for 30 minutes, filter out the fast plasma coagulation, and obtain the heme solution ( figure 1 shown);

[0037] The third step: adding disodium ethylenediaminetetraacetic acid to separate the complex, and then adding water separating agent to release heme.

[0038] Step 4: Dissolve the heme in toluene, add heavy potassium chromate to open the ring, extract with chloroform to obtain biliverdin; separate and discard the isomer biliverdin to obtain alpha biliverdin, add bisulfite Sodium, get alpha bilirubin (opening principle such as figure 2 shown); figure 2 Chinese letters indicate M:CH ...

Embodiment 2

[0045] Example 2: Step 1: Mix pig blood or bovine blood with 4% sodium citrate according to the mass ratio of 200:1, stir well and let stand for 12 hours to obtain hemoglobin solution;

[0046] Step 2: After adjusting the above solution to pH 2.0 with dilute hydrochloric acid, heat it at any temperature between 30-60 for 30 minutes, filter out the fast coagulation of plasma, and obtain heme solution ( figure 1 shown); the third step: add cyclohexanediaminetetraacetic acid to separate the complex, and then add water separating agent to release heme.

[0047] Step 4: Dissolve heme with xylene or cyclohexane, add hydrogen peroxide to open the ring, extract with chloroform to obtain biliverdin; separate and discard isomer biliverdin to obtain alpha biliverdin , Add sodium sulfite to get alpha bilirubin.

[0048] open loop principle figure 2 Shown:

[0049] M:CH 3 V:CH=CH 2 P:CH 2 -CH 2 -COOH

[0050] The fifth step: add saturated calcium hydroxide, let...

Embodiment 3

[0054] Example 3: Step 1: Mix pig blood or bovine blood with 4% sodium citrate according to the mass ratio of 200:1, stir evenly and let stand for 12 hours to obtain hemoglobin solution;

[0055] Step 2: After adjusting the above solution to pH 2.0 with dilute hydrochloric acid, heat it at any temperature between 30-60 for 30 minutes, filter out the fast coagulation of plasma, and obtain heme solution ( figure 1 shown);

[0056] The third step: adding cyclohexanediaminetetraacetic acid or disodium ethylenediaminetetraacetic acid to separate the complex, and then adding water separating agent or resin separating agent to release heme.

[0057] Step 4: Dissolve heme with xylene or cyclohexane, add hydrogen peroxide to open the ring, extract with chloroform to obtain biliverdin; separate and discard isomer biliverdin to obtain alpha biliverdin , add sodium sulfite to get alpha bilirubin,

[0058] Open loop schematic as figure 2 as shown, figure 2 Chinese letters indicate ...

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Abstract

The invention discloses a novel method for preparing calculus bovis factitius and raw materials thereof. The method comprises the following steps: weighing the following components in percentage by weight: 20-30 percent of calcium bilirubinate, 10-20 percent of sodium bilirubinate, 3-8 percent of taurine, 20-30 percent of deoxycholic acid, 2-5 percent of cholic acid or hyocholic acid, 2-5 percent of binding cholesterol or free cholesterol, 2-5 percent of zinc sulfate and 2-5 percent of magnesium sulfate; stirring for 20-60 minutes, adding the balance of ox gallbladder powder, and keeping on stirring for 20-60 minutes to obtain the calculus bovis factitius. According to the method for preparing calcium bilirubinate and sodium bilirubinate serving as effective components in the calculus bovis factitius, high-quality calculus bovis factitius is prepared according to a specific proportion, and the requirement of market for performance of calculus bovis factitius can be met.

Description

technical field [0001] The invention relates to a novel bezoar and a preparation method of its raw material. Background technique [0002] Natural bezoar is the dried gallstones of bovine animals, which are formed in the bile of cattle for several years. Natural bezoar is a famous and precious Chinese medicinal material, and there are hundreds of kinds of Chinese and Western medicines that need natural bezoar as a raw material. Due to the scarcity of sources of natural bezoar, it is far from being able to meet the needs. [0003] Artificial bezoar is usually made from animal bile outside the body of a cow. Since the 1950s, there have been researches on natural bezoar substitutes. In the 1960s, the first artificial bezoar was born. The artificial bezoar is composed of hyocholic acid, deoxycholic acid, free bilirubin, starch and the like. Since the 1970s, many scientific research institutions and scientific and technological workers in our country have invested in the rese...

Claims

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Application Information

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IPC IPC(8): A61K35/413
Inventor 张恒洲
Owner 张隶军
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