Serum-free CHO cell culture medium and preparation method thereof

Abstract

The invention discloses a serum-free CHO cell culture medium and a preparation method thereof. By adding such supplement factors with determined chemical ingredients as a variety of amino acids, vitamins, inorganic salts, trace elements, alkaloids, fatty acids and hormone and the like, the culture medium can be used for quickly growing CHO cells under a suspension culture condition without adaptation, the cell density and cell activity can be equal to those of a cell culture with serum, the CHO cell culture requirements can be satisfied and multiple unfavorable factors caused by the use of serum can be effectively avoided, the production cost is reduced and the serum-free CHO cell culture medium can be completely replacing the cell culture with serum to perform large scale culture and production of CHO cells. In addition, due to a powdery storage manner, the product transport risk can be effectively avoided, the transport cost can be reduced and the requirements of performing large scale culture on the CHO cells can be satisfied.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a CHO cell serum-free medium and a preparation method thereof. Background technique [0002] The serum in the traditional serum-containing medium brings many unfavorable factors to scientific research and production. The main function of serum is to provide cells with hormones, growth factors, transfer proteins and other nutrients needed for growth and proliferation, but it has many disadvantages: large batch-to-batch differences, a lot of verification work is required, inconvenient use, unclear components, There are growth-inhibiting components, which are especially not conducive to the separation and purification of target products such as vaccines and monoclonal antibodies, and are easily infected by viruses and mycoplasma; expensive serum may cause shortages in supply, and the source of serum is unstable. [0003] In recent years, many serum-free medium products have been develo...

AI Technical Summary

Problems solved by technology

[0002] The serum in the traditional serum-containing medium brings many unfavorable factors to scientific research and production

The main function of serum is to provide cells with hormones, growth factors, transfer proteins and other nutrients needed for growth and value-added, but it has many disadvantages: large batch-to-batch differences, a lot of verification work is required, inconvenient use, unclear components, There are growth-inhibiting components, which are especially unfavorable for the separation and purification of target products such as vaccines and monoclonal antibodies, and are easily infected by viruses and mycoplasma; expensive serum may cause shortages in supply, and the source of serum is unstable, etc.

[0003] In recent years, many serum-free medium products have been developed for the serum-free culture of CHO cells in China, but most of them are based on the existing DMEM, DMEM / F12, IMDM and other basic medium to ensure the activity of cells. Additives such as protein source, due to the complex composition, protein and its hydrolyzate will bring pollution in the cell culture process, affect the downstream separation and purification, and reduce the expression of the target product

[0004] The foreign commercial CHO cell serum-free medium has the following status quo: most of them are provided in the form of liquid, which is not suitable for large-scale production, expensive, and the composition of the medium is uncertain. Base, the price per liter is as high as thousands of yuan, which limits its practical application to a certain extent

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