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Method for combined production of bioethanol and pullulan from lignocellulose substances

A technology of lignocellulosic and pullulan sugar, which is applied in the field of biotransformation of fibrous substances, can solve the problems of ineffective utilization of xylose and high production cost of pullulan sugar, and achieve resource saving, production cost reduction, and high-efficiency biotransformation Effect

Active Publication Date: 2014-08-20
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems that xylose cannot be effectively utilized and the production cost of pullulan is too high in the current biotransformation process of cellulosic substances, and provides a method for co-producing ethanol and pullulan from lignocellulosic substances. method

Method used

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  • Method for combined production of bioethanol and pullulan from lignocellulose substances
  • Method for combined production of bioethanol and pullulan from lignocellulose substances
  • Method for combined production of bioethanol and pullulan from lignocellulose substances

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preparation example Construction

[0033] The preparation method of the corn flour hydrolyzate is as follows: add 1500 g of corn flour to 4500 mL of 65-70° C. tap water, and place for 20 minutes to make the corn flour particles fully absorb water and expand. Add 0.9mL of liquefaction enzyme (a-amylase), and liquefy at 85~90℃ for 1.5h. After the liquefaction is completed, the temperature is lowered to 60°C, and 3 mL of glucoamylase is added. Saccharification at 55-60°C for 20h. Filter the saccharification liquid with a filter cloth to obtain a clear filtrate, which is the corn hydrolysate, and boil it for 10 minutes to cool for later use.

[0034] (2) Genome rearrangement

[0035] All cell populations of the mutant strain library were inoculated into YPD liquid medium, cultured to logarithmic phase at 30°C and 180 rpm, and the cells were collected by centrifugation. Wash the bacteria with sterile water, then put the sludge into the pre-spore culture medium (10g / LKAc, 10g / L yeast powder, 20g / L peptone), culture at ...

Embodiment 1

[0051] Example 1: Preparation of bagasse hemicellulose hydrolysate

[0052] Remove the impurities in the bagasse and pulverize to 20-30 mesh, add 0.7% NaOH solution according to the solid-to-liquid ratio of 1:12 (g / mL), and pretreat it in the reactor for 1.5h at 40℃ . At this time, the retention rate of each component of bagasse is: 96.37% of cellulose, 81.02% of hemicellulose, 80.62% of lignin, and the yield of xylose is 62.93%. Wash the pretreated bagasse to neutrality, put the raw materials into the pH 4.5-5.5 enzymatic buffer aqueous solution according to the solid-liquid ratio of 1:18 (g / mL), and add the commercially available xylan according to the 150IU / g raw material Enzymes, while adding surfactant RH (rhamnolipid) 0.005%, Tween-800.25%, PEG60000.25%, Triton X-100 (Triton X-100) 0.15%, and enzymatically digest at 40℃ 48h. The yield of hemicellulose enzymatic hydrolysis was 98.52%%, which was 26.38% higher than that of enzymatic hydrolysis without surfactant. In the o...

Embodiment 2

[0053] Example 2: Extraction of lignin from bagasse and preparation of cellulose residue

[0054] After orthogonal optimization of the lignin extraction conditions, the optimal lignin extraction process is obtained: adding the solid residue obtained in Example 1 to the lignin extraction mixture, the solid-liquid ratio is 1:15 (g / mL), formic acid :Ethanol is 9:1 (v / v), the temperature is 90℃, and the reaction time is 2.5h. At this time, the extraction rate of lignin was 68.44%, and the yield of lignin was 49.61%. The cellulose content in the residue after lignin extraction is 68.16%, and the others are 22.22%.

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Abstract

The invention discloses a method for combined production of bioethanol and pullulan from lignocellulose substances. The method comprises the following steps: subjecting the lignocellulose substances to alkaline-process pretreatment and xylanase enzymatic hydrolysis and then carrying out solid-liquid separation so as to respectively obtain a hemicellulose hydrolysate (xylose liquid) and a solid-phase residue; subjecting the solid-phase residue to lignin extraction so as to obtain a cellulose residue; directly applying the xylose liquid to fermentation production of pullulan (wherein yield is no less than 0.29 g of pullulan per g of xylose) and subjecting the cellulose residue to synchronous saccharification and fermentation to produce ethanol; subjecting fermentation broth containing pullulan to fermentation and distillation with fiber residue, carrying out alcohol precipitation on obtained ethanol, carrying out extraction and refining so as to obtain a pullulan product, combining ethanol-containing waste liquid produced during purifying with cellulose residue ethanol fermentation broth and carrying out distillation so as to realize recovery and reuse of ethanol. The method can realize full utilization and high-value conversion of hemicellulose and cellulose, saves resources and achieves the purposes of environmental protection and economic benefit improvement.

Description

Technical field: [0001] The invention belongs to the technical field of fibrous material biotransformation, and in particular relates to a method for using lignocellulosic materials to jointly produce ethanol and pullulan. Background technique: [0002] Lignocellulosic substances are composed of cellulose, hemicellulose, and lignin. It is the largest and most widely distributed renewable resource on earth. In recent years, with the increasing depletion of mineral fuels, the utilization of lignocellulosic materials has received widespread attention. my country is a large traditional agricultural country, and the content of cellulose is quite abundant. However, these resources have not been used rationally for a long time. Agricultural wastes rich in cellulose such as straw, bagasse, corn cobs, and straw are generally burned or discarded directly. This primitive treatment method not only caused a huge waste of resources. , And will bring great pollution to the environment. The u...

Claims

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Application Information

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IPC IPC(8): C12P7/08C12P19/14C12P19/10C12R1/645C12R1/865
Inventor 陈叶福肖冬光郭建董健李月强郭学武
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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