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Preparation method for mesoporous-silicon medicine-carrying system modified by bone-morphogenetic-protein active polypeptide

A technology of morphogenic proteins and active polypeptides, applied in prosthetics, medical science, etc., can solve the problems of complex structure, limited wide application, limited number of BMP proteins, etc., and achieve good dispersion effect

Inactive Publication Date: 2014-08-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, natural BMP proteins are limited in number, complex in structure, and rapidly degraded in vivo
As an exogenous growth factor, BMP has a very short half-life in the body, and the action of enzymes in the body will inactivate it, which limits its wide application

Method used

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  • Preparation method for mesoporous-silicon medicine-carrying system modified by bone-morphogenetic-protein active polypeptide
  • Preparation method for mesoporous-silicon medicine-carrying system modified by bone-morphogenetic-protein active polypeptide
  • Preparation method for mesoporous-silicon medicine-carrying system modified by bone-morphogenetic-protein active polypeptide

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Experimental program
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Effect test

Embodiment 1

[0034] (1) The preparation of mesoporous silicon nanoparticles refers to the literature method (J.Am.Chem.Soc., 2004, 126, 13216-13217). The synthesis steps are as follows: Add 1 g of cetyltrimethylammonium bromide (CTAB) into a beaker containing 480 ml of deionized water, add 3.5 ml of 2mol / L NaOH solution, and control the temperature of the oil bath at 80°C. After the solution was clarified, 5ml of tetraethyl orthosilicate (TEOS) was added, and a white precipitate was produced after 2 hours of reaction. The product was collected by centrifugation (8000 rpm) and washed 3 times with deionized water and ethanol. The template agent CTAB was removed by refluxing in methanolic acid solution (160ml methanol and 9ml hydrochloric acid) for 24 hours, then centrifuged and freeze-dried to obtain mesoporous silicon nanoparticles without template agent;

[0035] (2) Evenly disperse the mesoporous silicon nanoparticles obtained in step (1) in ethanol, add 3-aminopropyltriethoxysilane, rea...

Embodiment 2

[0043] (1) The preparation of mesoporous silicon nanoparticles refers to the literature method (J.Am.Chem.Soc., 2004, 126, 13216-13217). The synthesis steps are as follows: Add 1 g of cetyltrimethylammonium bromide (CTAB) into a beaker containing 480 ml of deionized water, add 3.5 ml of 2mol / L NaOH solution, and control the temperature of the oil bath at 80°C. After the solution was clarified, 5ml of tetraethyl orthosilicate (TEOS) was added, and a white precipitate was produced after 2 hours of reaction. The product was collected by centrifugation (8000 rpm) and washed 3 times with deionized water and ethanol. The template agent CTAB was removed by refluxing in methanolic acid solution (160ml methanol and 9ml hydrochloric acid) for 24 hours, then centrifuged and freeze-dried to obtain mesoporous silicon nanoparticles without template agent;

[0044] (2) Evenly disperse the mesoporous silicon nanoparticles obtained in step (1) in ethanol, add 3-aminopropyltriethoxysilane, rea...

Embodiment 3

[0052] (1) The preparation of mesoporous silicon nanoparticles refers to the literature method (J.Am.Chem.Soc., 2004, 126, 13216-13217). The synthesis steps are as follows: Add 1 g of cetyltrimethylammonium bromide (CTAB) into a beaker containing 480 ml of deionized water, add 3.5 ml of 2mol / L NaOH solution, and control the temperature of the oil bath at 80°C. After the solution was clarified, 5ml of tetraethyl orthosilicate (TEOS) was added, and a white precipitate was produced after 2 hours of reaction. The product was collected by centrifugation (8000 rpm) and washed 3 times with deionized water and ethanol. The template agent CTAB was removed by refluxing in methanolic acid solution (160ml methanol and 9ml hydrochloric acid) for 24 hours, then centrifuged and freeze-dried to obtain mesoporous silicon nanoparticles without template agent;

[0053] (2) Evenly disperse the mesoporous silicon nanoparticles obtained in step (1) in ethanol, add 3-aminopropyltriethoxysilane, rea...

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Abstract

A preparation method for a mesoporous-silicon medicine-carrying system modified by bone-morphogenetic-protein active polypeptide comprises: dispersing mesporous silicon into an organic solvent, adding a silane coupling agent 3- aminopropyltriethoxysilane, mixing and reacting to obtain aminated mesoporous silicon; utilizing a cross-linking agent to perform covalent grafting on bone-morphogenetic-protein active polypeptide and aminated mesoporous silicon; utilizing a cross-linking agent to react polyethylene glycol monocarboxylic acid with residual amino on mesoporous silicon; and loading with medicine molecules capable of realizing induction ossification. The method is easy to operate, simple in equipment and mild in reaction conditions, and price is low. The prepared mesoporous silicon medicine-carrying particles are good in dispersibility, good in stability and good in biological compatibility. The medicine-carry system comprises bone-morphogenetic-protein active polypeptide and the medicine molecules both containing bone induction activity, and is applicable to bone defect restoration.

Description

technical field [0001] The invention belongs to the field of preparation of bone repair materials, in particular to a preparation method of a mesoporous silicon drug-carrying system modified by a bone morphogenetic protein active polypeptide. Background technique [0002] Bone is a hard tissue and organ in the human body, which shoulders important responsibilities for movement, support and protection of the body. It is estimated that there are millions of patients with bone defects or dysfunction in my country every year due to bone trauma caused by traffic accidents and production safety accidents, spinal degenerative diseases, tumor bone resection and other reasons. Therefore, bone defect has become a common bone tissue disease in daily medical activities. [0003] At present, bone tissue transplantation and artificial bone repair materials are the main treatment methods for bone tissue repair. Among them, artificial bone repair materials can not only overcome the defect...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/18A61L27/22A61L27/54
Inventor 何创龙周小军冯炜仇可新聂伟陈良
Owner DONGHUA UNIV
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