Melatonin rapid detection method, detection card and preparation method thereof

Abstract

The invention belongs to the technical field of biological detection, discloses a rapid melatonin detection method, a melatonin detection card and a preparation method of the detection card, and aims to provide a rapid detection method which is convenient to use, rapid, simple and high in sensitivity and used for detecting melatonin doped in food, health food or medicine and a detection card used by the method. According to the technical scheme, 1), an organic solvent is added to a product required to be detected for dissolution, filtering is performed after a slow-release preparation is added for dilution, and a filtering liquid is collected; 2), the filtering liquid collected from the step 1) is dropwise added to a melatonin colloidal gold chromatographic assay card, positive or negative of a to-be-detected sample is judged, the to-be-detected sample is negative if a control line C and a test line T are purple red, and the to-be-detected sample is positive if the control line C is purple red and the test line T doesn't color; and if the control line C and the test line T on colloidal gold test paper both don't color or only the test line colors, the experiment is invalid.

Description

technical field [0001] The invention relates to a detection method, specifically a rapid detection method and a kit for melatonin, which belong to the technical field of biological detection. Background technique [0002] Melatonin, also known as pineal gland, chemically named N-acetyl-5-methoxytryptamine, is an indole amine hormone secreted by the pineal gland in the human brain. Its secretion decreases with the increase of light, so it is also called dark hormone. Melatonin is mainly used to regulate the body's wakefulness and sleep cycle, induce physiological sleep, and anti-aging. [0003] Melatonin is an endogenous hormone. Studies have shown that excessive use of melatonin may cause respiratory problems and produce a hangover effect. At the same time, melatonin can affect the effect of certain sedative and hypnotic drugs such as clonazepam. In Europe, melatonin's taking cycle, applicable population and dosage are strictly limited. In the United States, melatonin is ...

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: the chromatographic resolution is low, the composition of Chinese patent medicine is complicated, there are many interference factors, and it is easy to cause misjudgment

However, there are still the following disadvantages at present: the instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high; when the chromatographic peak retention time of the coexisting components is close to the melatonin chromatographic retention time, it is easy to cause misjudgment; It has high requirements on the environment and needs to be placed fixedly, so it is not suitable for on-site inspection

At present, these tests can only be carried out in a small number of laboratories and are not easy to promote and use

[0020] To sum up, at present, in the detection of melatonin adulteration in Chinese patent medicines and health foods, although there are rapid screening methods for physical and chemical reactions suitable for on-site detection, there is still a sensitivity, simple operation, and rapid screening. The method of melatonin, for pharmacies and basic drug inspection units, develops a rapid screening method for illegal adulteration of melatonin in Chinese patent medicines and health foods, conducts rapid analysis of samples at the supervision site, and provides timely information for the supervision of Chinese patent medicines and health foods evidence, it is necessary to crack down on counterfeiting by lawbreakers and ensure the safety of medication for the people

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