Hansenula polymorpha recombination strain and application thereof in biosynthesis of gentiopicroside

A technology of Hansenula polymorpha and recombinant strains, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., to achieve the effect of easy separation and extraction, and high-density fermentation

Active Publication Date: 2014-08-06
SHAANXI UNIV OF SCI & TECH
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  • Hansenula polymorpha recombination strain and application thereof in biosynthesis of gentiopicroside
  • Hansenula polymorpha recombination strain and application thereof in biosynthesis of gentiopicroside
  • Hansenula polymorpha recombination strain and application thereof in biosynthesis of gentiopicroside

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0026] The present invention uses H.polymorpha DL-1 as the starting strain (the source is ATCC No.26012), utilizes low-energy ion irradiation injection to mediate the random transformation of Gentiana genomic DNA into yeast, and obtains recombinant yeast producing gentiopicroside through qualitative and quantitative screening Strains, and their genetic stability and corresponding fermentation tests.

[0027] (1) culture medium

[0028] The composition of YPD liquid medium is: glucose 20.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, pH 6.5;

[0029] The composition of YPD solid medium is: glucose 20.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, agar 20g / L, pH 6.5.

[0030] (2) The method on which the screening is based

[0031] (1) Detection of iridoid glycosides

[0032] Molish reaction: take 800mL of fermentation broth, concentrate the fermentati...

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Abstract

The invention provides a hansenula polymorpha recombination strain and application thereof in the biosynthesis of gentiopicroside. A recombination saccharomycete used for producing gentiopicroside is obtained by a method for conversion of low energy ion implantation mediated gentiana macrophylla genome total DNA in the saccharomycete, and is named as hansenula polymorpha of which the preservation number is CGMCC.No.8998. The hansenula polymorpha recombination strain and the application realize the technical breakthrough in the aspect of using the recombination saccharomycete to be subjected to biological fermentation to produce gentiopicroside, solves the source shortage of gentiopicroside, protects the gentianaceae plant resources of our country, and provides a new way for sustainable development of the gentianaceae plant resources.

Description

technical field [0001] The invention belongs to the field of transgenic yeast recombinant strains producing gentiopicroside obtained through genetic transformation, in particular to Hansenula polymorpha recombinant strains obtained by using ion implantation to mediate the genetic transformation of Gentiana total DNA in yeast and its production of gentiopicroside Applications in glycoside biosynthesis. Background technique [0002] Gentiopicroside, also known as Gentiopicroside, the molecular formula is C 16 h 20 o 9 , with a molecular weight of 356.11, is a split ring terpene glycoside compound, which belongs to the sesquiterpenes, and is the main medicinal component of the medicinal plant Gentiana macrophylla. Studies in recent years have shown that gentiopicroside has analgesic, anti-inflammatory, antibacterial, anti-viral, anti-oxidative, liver-protecting and choleretic, stomach-strengthening and anti-ulcer effects. It is the freeze-dried powder injection of gentiopic...

Claims

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Application Information

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IPC IPC(8): C12N1/19C12P19/60C12R1/78
Inventor 钱卫东付云芳蔡长龙毛培宏王婷赵德志施春阳
Owner SHAANXI UNIV OF SCI & TECH
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