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A kind of nitrogen-fixing bacteria rhizobium strain scaus152 and its application

A technology of nitrogen fixation and bacterial strains, which is applied in the field of microorganisms, can solve the problems of small planting area, scattered planting, and low yield, and achieve the effects of strong nitrogen fixation ability, good affinity, and strong stress resistance

Inactive Publication Date: 2016-05-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on excellent rhizobium bacteria that match the soybean varieties and ecological environment in the southern soybean producing area, Sichuan soybean producing area.
[0005] Soybean in Sichuan has always been a minor crop, with scattered planting, low yield and small planting area
At present, no excellent soybean rhizobia that matches the main soybean varieties in Sichuan and is suitable for different ecological environments in Sichuan has not been screened out.

Method used

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  • A kind of nitrogen-fixing bacteria rhizobium strain scaus152 and its application
  • A kind of nitrogen-fixing bacteria rhizobium strain scaus152 and its application
  • A kind of nitrogen-fixing bacteria rhizobium strain scaus152 and its application

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Experimental program
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Effect test

Embodiment 1

[0015] Isolation, purification and preservation of embodiment 1 rhizobia

[0016] From the soybeans planted in the low mountain and hilly area of ​​Yanyuan County, Liangshan Prefecture, Sichuan Province, select the large and full red root nodules on the taproots of robust plants, scrub the root nodules clean, pick off part of the root bark, dry them with paper, and put them away. In a vial filled with anhydrous calcium chloride and covered with absorbent cotton. After soaking the collected root nodules in sterile water for imbibition in the laboratory, soak in 95% ethanol for 30 seconds to eliminate surface tension, then disinfect the surface with 0.1% (m / v) mercury chloride for 5 minutes, and then rinse with sterile water 6 times, in the case of aseptic operation, a single root nodule was clipped and then added with Congo red YAM medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O0.2g, CaCl 2 .6H 2 O0.1g, NaCl0.1g, 1% (m / v) sodium molybdate 2ml, 1% ...

Embodiment 2

[0019] Back grafting and matching test of embodiment 2 rhizobia

[0020] The backgrafting test of rhizobium was carried out in a hydroponics method. The soybean variety used in the test was "Nandou 12", a leading variety in Sichuan. It was carried out in a light room (temperature control 22-24°C, light intensity 2700-3000 lux, and sunshine time 14h). , planted 46d to harvest. After successful back-grafting with "Nandou 12", the compatibility test with other main soybean varieties was carried out, and the hydroponics method was also carried out. The soybean variety used in the test: another main soybean variety "Gongxuan 1" for intercropping ", the spring soybean variety "Chuandou 14", and the main fresh soybean variety "Chunfengzao" in the Panxi area. After being cultivated in the above-mentioned light chamber for 1 week, they were cultured under normal light and temperature conditions, and harvested 41 days after planting. Regularly supplement sterile micro-nitrogen nutrien...

Embodiment 3

[0030] Stress resistance of nitrogen-fixing bacterium rhizogenes strain SCAUs152 described in embodiment 3

[0031] The stress resistance of the nitrogen-fixing bacteria strain SCAUs152 was mainly measured by acid and alkali tolerance, salt tolerance and growth in the greenhouse. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control. The YMA slant culture of the above-mentioned nitrogen-fixing bacteria strain SCAUs152 was scraped and washed with sterile water for use. The point inoculation method was adopted and repeated 3 times. YMA medium was used as the basal medium for the determination of acid and alkali resistance, and the pH value was adjusted with HCl and NaOH. The pH value was 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0, and 12.0. Salt tolerance determination also uses YMA medium as the base medium, and the strains are spot-inoculated on a plate containing NaCl, and the mass volume fraction of Na...

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Abstract

The invention discloses an Azorhizobium strain SCAUs152 and application thereof. The strain is a new strain which is separated and purified from fresh soybean root nodules and belongs to Rhizobium. The strain is collected to China Center for Type Culture Collection of Wuhan University on March 14th, 2014, and the collection number is CCTCC NO.M2014085. The strain is applied to production of Sichuan soybeans. The Azorhizobium strain SCAUs152 is a favorable broad-spectrum Rhizobium japonicum strain with high symbiotic fixation capacity, wide application range of Sichuan soybean species, IAA (indoleacetic acid) secretion capacity, capacities for dissolving inorganic phosphorus, organic phosphorus and potassium and high stress tolerance. The strain has favorable matching affinity with Sichuan dominant bean species, and can enhance the soybean yield by more than 23% in different ecological regions where no nitrogen fertilizer is applied and the Azorhizobium strain SCAUs152 is inoculated, which is much different from the regions where the Azorhizobium strain SCAUs152 is not inoculated.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a nitrogen-fixing bacteria strain SCAUs152 and its application. Background technique [0002] Chemical nitrogen fertilizers have made great contributions to increasing crop yields, but they have high production costs, low utilization rates, and pollute the ecological environment. Biological nitrogen fixation has the advantages of low cost, large amount of nitrogen fixation, continuous nitrogen fixation process, and no pollution, which is conducive to the protection of the ecological environment and the sustainable development of agriculture, and is an effective means of saving costs and increasing efficiency in agricultural production. Great efforts are needed to develop environmentally friendly biological nitrogen fixation technologies in food production. The rhizobia-legume nitrogen fixation system is the most efficient in biological nitrogen fixation. Artificial inoculation of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00A01G31/00C12R1/41
Inventor 陈远学徐开未周涛邹兰熊峰李娜杨昱
Owner SICHUAN AGRI UNIV
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